The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants.
Bacteria live primarily in microbial communities (biofilms), where they exhibit considerably higher biocide tolerance than their planktonic counterparts. Current standardized efficacy testing protocols of disinfectants, however, employ predominantly planktonic bacteria. In order to test the efficacy...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4912112?pdf=render |
| _version_ | 1811209112273289216 |
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| author | Katharina Konrat Ingeborg Schwebke Michael Laue Christin Dittmann Katja Levin Ricarda Andrich Mardjan Arvand Christoph Schaudinn |
| author_facet | Katharina Konrat Ingeborg Schwebke Michael Laue Christin Dittmann Katja Levin Ricarda Andrich Mardjan Arvand Christoph Schaudinn |
| author_sort | Katharina Konrat |
| collection | DOAJ |
| description | Bacteria live primarily in microbial communities (biofilms), where they exhibit considerably higher biocide tolerance than their planktonic counterparts. Current standardized efficacy testing protocols of disinfectants, however, employ predominantly planktonic bacteria. In order to test the efficacy of biocides on biofilms in a standardized manner, a new assay was developed and optimized for easy-handling, quickness, low running costs, and above all-repeatability. In this assay, 5 mm glass- or polytetrafluoroethylene beads in 24 well microtiter plates served as substrate for Pseudomonas aeruginosa biofilms. After optimizing result-relevant steps, the actual performance of the assay was explored by treating P. aeruginosa biofilms with glutaraldehyde, isopropanol, or peracetic acid in predefined concentrations. The aspired 5 log10 reduction in CFU counts was achieved by glutaraldehyde at 5% (30 min), and by peracetic acid at 0.3% (10 min). In contrast, 80% isopropanol (30 min) failed to meet the reduction goal. However, the main accomplishment of this study was to unveil the potential of the array itself; most noteworthy here, a reliable repeatability of the results. The new bead assay for biofilms is a robust, quick and cost-effective method for assessing the efficacy of biocides against biofilms. |
| first_indexed | 2024-04-12T04:33:56Z |
| format | Article |
| id | doaj.art-d9c05fa3f51147e794186422c052c829 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:33:56Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d9c05fa3f51147e794186422c052c8292022-12-22T03:47:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01116e015766310.1371/journal.pone.0157663The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants.Katharina KonratIngeborg SchwebkeMichael LaueChristin DittmannKatja LevinRicarda AndrichMardjan ArvandChristoph SchaudinnBacteria live primarily in microbial communities (biofilms), where they exhibit considerably higher biocide tolerance than their planktonic counterparts. Current standardized efficacy testing protocols of disinfectants, however, employ predominantly planktonic bacteria. In order to test the efficacy of biocides on biofilms in a standardized manner, a new assay was developed and optimized for easy-handling, quickness, low running costs, and above all-repeatability. In this assay, 5 mm glass- or polytetrafluoroethylene beads in 24 well microtiter plates served as substrate for Pseudomonas aeruginosa biofilms. After optimizing result-relevant steps, the actual performance of the assay was explored by treating P. aeruginosa biofilms with glutaraldehyde, isopropanol, or peracetic acid in predefined concentrations. The aspired 5 log10 reduction in CFU counts was achieved by glutaraldehyde at 5% (30 min), and by peracetic acid at 0.3% (10 min). In contrast, 80% isopropanol (30 min) failed to meet the reduction goal. However, the main accomplishment of this study was to unveil the potential of the array itself; most noteworthy here, a reliable repeatability of the results. The new bead assay for biofilms is a robust, quick and cost-effective method for assessing the efficacy of biocides against biofilms.http://europepmc.org/articles/PMC4912112?pdf=render |
| spellingShingle | Katharina Konrat Ingeborg Schwebke Michael Laue Christin Dittmann Katja Levin Ricarda Andrich Mardjan Arvand Christoph Schaudinn The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants. PLoS ONE |
| title | The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants. |
| title_full | The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants. |
| title_fullStr | The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants. |
| title_full_unstemmed | The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants. |
| title_short | The Bead Assay for Biofilms: A Quick, Easy and Robust Method for Testing Disinfectants. |
| title_sort | bead assay for biofilms a quick easy and robust method for testing disinfectants |
| url | http://europepmc.org/articles/PMC4912112?pdf=render |
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