Determination of Intracellular Osmolytes in Cyanobacterial Cells
Most of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol...
| मुख्य लेखकों: | , , , |
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| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
Bio-protocol LLC
2018-04-01
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| श्रृंखला: | Bio-Protocol |
| ऑनलाइन पहुंच: | https://bio-protocol.org/en/bpdetail?id=2812&type=0 |
| _version_ | 1831868991177490432 |
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| author | Xiaoming Tan Kuo Song Cuncun Qiao Xuefeng Lu |
| author_facet | Xiaoming Tan Kuo Song Cuncun Qiao Xuefeng Lu |
| author_sort | Xiaoming Tan |
| collection | DOAJ |
| description | Most of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol was applicable to at least four kinds of cyanobacteria, filamentous cyanobacterium Anabaena sp. PCC 7120, unicellular cyanobacterium Synechocystis sp. PCC 6803, Synechococcus elongatus PCC 7942 and halotolerant unicellular cyanobacterium Synechococcus sp. PCC 7002. |
| first_indexed | 2025-03-21T19:03:16Z |
| format | Article |
| id | doaj.art-d9c25040435a44aa853180ceb51d0af2 |
| institution | Directory Open Access Journal |
| issn | 2331-8325 |
| language | English |
| last_indexed | 2025-03-21T19:03:16Z |
| publishDate | 2018-04-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj.art-d9c25040435a44aa853180ceb51d0af22024-06-05T01:57:45ZengBio-protocol LLCBio-Protocol2331-83252018-04-018810.21769/BioProtoc.2812Determination of Intracellular Osmolytes in Cyanobacterial CellsXiaoming Tan0Kuo Song1Cuncun Qiao2Xuefeng Lu3Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaKey Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaMost of the cyanobacteria accumulate osmolytes including sucrose, glucosylglycerol, in their cells in response to salt stress. Here we describe a protocol of our laboratory for extraction and quantification of cyanobacterial intracellular sucrose and glucosylglycerol. We have confirmed this protocol was applicable to at least four kinds of cyanobacteria, filamentous cyanobacterium Anabaena sp. PCC 7120, unicellular cyanobacterium Synechocystis sp. PCC 6803, Synechococcus elongatus PCC 7942 and halotolerant unicellular cyanobacterium Synechococcus sp. PCC 7002.https://bio-protocol.org/en/bpdetail?id=2812&type=0 |
| spellingShingle | Xiaoming Tan Kuo Song Cuncun Qiao Xuefeng Lu Determination of Intracellular Osmolytes in Cyanobacterial Cells Bio-Protocol |
| title | Determination of Intracellular Osmolytes in Cyanobacterial Cells |
| title_full | Determination of Intracellular Osmolytes in Cyanobacterial Cells |
| title_fullStr | Determination of Intracellular Osmolytes in Cyanobacterial Cells |
| title_full_unstemmed | Determination of Intracellular Osmolytes in Cyanobacterial Cells |
| title_short | Determination of Intracellular Osmolytes in Cyanobacterial Cells |
| title_sort | determination of intracellular osmolytes in cyanobacterial cells |
| url | https://bio-protocol.org/en/bpdetail?id=2812&type=0 |
| work_keys_str_mv | AT xiaomingtan determinationofintracellularosmolytesincyanobacterialcells AT kuosong determinationofintracellularosmolytesincyanobacterialcells AT cuncunqiao determinationofintracellularosmolytesincyanobacterialcells AT xuefenglu determinationofintracellularosmolytesincyanobacterialcells |