Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology
Hypo-osmotic swelling test (HOST) is used to assess the functional integrity of sperm plasma membranes in many species. The primary aim of this study was to test a simplified HOST procedure for the evaluation of feline semen. The second objective was to check if sperm abnormalities can influence the...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/7/903 |
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| author | Sylwia Prochowska Wojciech Niżański Alain Fontbonne |
| author_facet | Sylwia Prochowska Wojciech Niżański Alain Fontbonne |
| author_sort | Sylwia Prochowska |
| collection | DOAJ |
| description | Hypo-osmotic swelling test (HOST) is used to assess the functional integrity of sperm plasma membranes in many species. The primary aim of this study was to test a simplified HOST procedure for the evaluation of feline semen. The second objective was to check if sperm abnormalities can influence the results of this test. Urethral semen was collected from 19 male, domestic cats. In Exp. 1, HOST was performed in different media (50 mOsm/kg fructose or distilled water), temperature (37 °C or room temperature) and time (5 and 30 min). In Exp. 2, the potential effect of sperm abnormalities on HOST results was assessed by observing individual normal and abnormal spermatozoa microinjected into droplets of distilled water. The results showed no differences between the HOST results performed in different media, temperature and time. Viable abnormal spermatozoa were able to swell under hypo-osmotic conditions in the same manner as normal ones, except spermatozoa with distal droplets, which showed a higher frequency of ‘despiralization’. In conclusion, HOST can be reliably performed at 0 mOsm/kg for 5 min at room temperature, which may contribute to a wider use of this test under clinical environments. Viable abnormal spermatozoa are able to swell under hypo-osmotic conditions; therefore, their presence in the ejaculate would not bias the results of HOST when total coiling is calculated. |
| first_indexed | 2024-03-09T12:11:09Z |
| format | Article |
| id | doaj.art-d9c390119bf642109daee7cac41612ed |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T12:11:09Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-d9c390119bf642109daee7cac41612ed2023-11-30T22:51:59ZengMDPI AGAnimals2076-26152022-03-0112790310.3390/ani12070903Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm MorphologySylwia Prochowska0Wojciech Niżański1Alain Fontbonne2Department of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 49, 50-366 Wrocław, PolandDepartment of Reproduction and Clinic of Farm Animals, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 49, 50-366 Wrocław, PolandÉcole Nationale Vétérinaire d’Alfort, 7 Avenue du Général de Gaulle, 94700 Maisons-Alfort, FranceHypo-osmotic swelling test (HOST) is used to assess the functional integrity of sperm plasma membranes in many species. The primary aim of this study was to test a simplified HOST procedure for the evaluation of feline semen. The second objective was to check if sperm abnormalities can influence the results of this test. Urethral semen was collected from 19 male, domestic cats. In Exp. 1, HOST was performed in different media (50 mOsm/kg fructose or distilled water), temperature (37 °C or room temperature) and time (5 and 30 min). In Exp. 2, the potential effect of sperm abnormalities on HOST results was assessed by observing individual normal and abnormal spermatozoa microinjected into droplets of distilled water. The results showed no differences between the HOST results performed in different media, temperature and time. Viable abnormal spermatozoa were able to swell under hypo-osmotic conditions in the same manner as normal ones, except spermatozoa with distal droplets, which showed a higher frequency of ‘despiralization’. In conclusion, HOST can be reliably performed at 0 mOsm/kg for 5 min at room temperature, which may contribute to a wider use of this test under clinical environments. Viable abnormal spermatozoa are able to swell under hypo-osmotic conditions; therefore, their presence in the ejaculate would not bias the results of HOST when total coiling is calculated.https://www.mdpi.com/2076-2615/12/7/903cathypo-osmotic swelling testwater testabnormal spermatozoaclinical practice |
| spellingShingle | Sylwia Prochowska Wojciech Niżański Alain Fontbonne Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology Animals cat hypo-osmotic swelling test water test abnormal spermatozoa clinical practice |
| title | Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology |
| title_full | Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology |
| title_fullStr | Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology |
| title_full_unstemmed | Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology |
| title_short | Hypo-Osmotic Swelling Test (HOST) for Feline Spermatozoa: The Simplified Procedure and the Aspect of Sperm Morphology |
| title_sort | hypo osmotic swelling test host for feline spermatozoa the simplified procedure and the aspect of sperm morphology |
| topic | cat hypo-osmotic swelling test water test abnormal spermatozoa clinical practice |
| url | https://www.mdpi.com/2076-2615/12/7/903 |
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