Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition
Summary: Arterial diseases continue to pose a major health concern but in vitro studies are limited because explanted cells can exhibit poor proliferative capacity and a loss of specificity. Here, we find that two transcription factors, MYCN and SOX17, induce and indefinitely expand in culture precu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671117305593 |
| _version_ | 1818204044675514368 |
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| author | Auston Z. Miller Alexander Satchie Alex P. Tannenbaum Aman Nihal James A. Thomson David T. Vereide |
| author_facet | Auston Z. Miller Alexander Satchie Alex P. Tannenbaum Aman Nihal James A. Thomson David T. Vereide |
| author_sort | Auston Z. Miller |
| collection | DOAJ |
| description | Summary: Arterial diseases continue to pose a major health concern but in vitro studies are limited because explanted cells can exhibit poor proliferative capacity and a loss of specificity. Here, we find that two transcription factors, MYCN and SOX17, induce and indefinitely expand in culture precursors of human arterial endothelial cells (expandable arterial endothelial precursors [eAEPs]). The eAEPs are derived from CD34+ cells found in umbilical cord blood or adult bone marrow. Independent eAEP lines differ in their proclivity to undergo an endothelial-to-mesenchymal transition (EndoMT), a hallmark event in a broad array of vascular diseases and disorders. Some cell lines spontaneously become mesenchymal over time in culture, an effect exacerbated by inhibition of the fibroblast growth factor receptor, while others do not readily convert. These distinctions were exploited to identify genes that correlate with resistance to an EndoMT and to elucidate transcriptional changes that underpin the transition. |
| first_indexed | 2024-12-12T03:34:59Z |
| format | Article |
| id | doaj.art-d9c44a1aad6b4790860c335842d05648 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-12T03:34:59Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-d9c44a1aad6b4790860c335842d056482022-12-22T00:39:49ZengElsevierStem Cell Reports2213-67112018-01-01101738610.1016/j.stemcr.2017.12.011Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal TransitionAuston Z. Miller0Alexander Satchie1Alex P. Tannenbaum2Aman Nihal3James A. Thomson4David T. Vereide5Morgridge Institute for Research, Madison, WI 53715, USAMorgridge Institute for Research, Madison, WI 53715, USAMorgridge Institute for Research, Madison, WI 53715, USAMorgridge Institute for Research, Madison, WI 53715, USAMorgridge Institute for Research, Madison, WI 53715, USA; Department of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA; Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USAMorgridge Institute for Research, Madison, WI 53715, USA; Corresponding authorSummary: Arterial diseases continue to pose a major health concern but in vitro studies are limited because explanted cells can exhibit poor proliferative capacity and a loss of specificity. Here, we find that two transcription factors, MYCN and SOX17, induce and indefinitely expand in culture precursors of human arterial endothelial cells (expandable arterial endothelial precursors [eAEPs]). The eAEPs are derived from CD34+ cells found in umbilical cord blood or adult bone marrow. Independent eAEP lines differ in their proclivity to undergo an endothelial-to-mesenchymal transition (EndoMT), a hallmark event in a broad array of vascular diseases and disorders. Some cell lines spontaneously become mesenchymal over time in culture, an effect exacerbated by inhibition of the fibroblast growth factor receptor, while others do not readily convert. These distinctions were exploited to identify genes that correlate with resistance to an EndoMT and to elucidate transcriptional changes that underpin the transition.http://www.sciencedirect.com/science/article/pii/S2213671117305593self-renewalarterial endothelial precursorsendothelial-to-mesenchymal transitionMYCNSOX17transcription factors |
| spellingShingle | Auston Z. Miller Alexander Satchie Alex P. Tannenbaum Aman Nihal James A. Thomson David T. Vereide Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition Stem Cell Reports self-renewal arterial endothelial precursors endothelial-to-mesenchymal transition MYCN SOX17 transcription factors |
| title | Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition |
| title_full | Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition |
| title_fullStr | Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition |
| title_full_unstemmed | Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition |
| title_short | Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition |
| title_sort | expandable arterial endothelial precursors from human cd34 cells differ in their proclivity to undergo an endothelial to mesenchymal transition |
| topic | self-renewal arterial endothelial precursors endothelial-to-mesenchymal transition MYCN SOX17 transcription factors |
| url | http://www.sciencedirect.com/science/article/pii/S2213671117305593 |
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