Effects of bisphenol A on uterine leiomyoma: In vitro and in vivo evaluation with mechanistic insights related to XBP1
The incidence rate of human uterine leiomyomas is over 70% in the women of childbearing age, which has caused serious health and financial burden. Our previous study confirmed that Bisphenol A (BPA)，representative environmental estrogen, promoted the proliferation of human uterine leiomyomas and up-...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322010417 |
| _version_ | 1828098389795930112 |
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| author | Zemin Li Han Yin Kai Chen Bo Ding Jingyun Xu Mulan Ren Chuan Zhang Yang Shen |
| author_facet | Zemin Li Han Yin Kai Chen Bo Ding Jingyun Xu Mulan Ren Chuan Zhang Yang Shen |
| author_sort | Zemin Li |
| collection | DOAJ |
| description | The incidence rate of human uterine leiomyomas is over 70% in the women of childbearing age, which has caused serious health and financial burden. Our previous study confirmed that Bisphenol A (BPA)，representative environmental estrogen, promoted the proliferation of human uterine leiomyomas and up-regulated the expression of cell proliferation-related genes. In this study, by combining ChIP-seq and RNA-seq, it was shown that after BPA intervention, H3K27ac modification levels and gene expression levels were altered in uterine leiomyomas cells. Moreover experimental verification found that BPA can regulate ITGA2 through the transcription factor XBP1, activate the downstream PI3K/AKT signaling pathway, eventually promote the proliferation of uterine leiomyomas. The present study provides new insights into the pathogenesis associated with exposure to BPA and other endocrine disruptors with similar effects by defining XBP1 as an important regulator, and which may act as an intervention and treatment target for uterine leiomyomas. |
| first_indexed | 2024-04-11T08:02:01Z |
| format | Article |
| id | doaj.art-d9c522ed317247de940fb5281e359cd6 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T08:02:01Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-d9c522ed317247de940fb5281e359cd62022-12-22T04:35:42ZengElsevierEcotoxicology and Environmental Safety0147-65132022-12-01247114201Effects of bisphenol A on uterine leiomyoma: In vitro and in vivo evaluation with mechanistic insights related to XBP1Zemin Li0Han Yin1Kai Chen2Bo Ding3Jingyun Xu4Mulan Ren5Chuan Zhang6Yang Shen7Department of Obstetrics and Gynecology Zhongda Hospital, Southeast University, Nanjing, ChinaDepartment of Obstetrics and Gynecology Zhongda Hospital, Southeast University, Nanjing, ChinaNovant Health Maternal Fetal Medicine, Winston-Salem, NC 27103, USADepartment of Obstetrics and Gynecology Zhongda Hospital, Southeast University, Nanjing, ChinaDepartment of Obstetrics and Gynecology Zhongda Hospital, Southeast University, Nanjing, ChinaDepartment of Obstetrics and Gynecology Zhongda Hospital, Southeast University, Nanjing, ChinaNational Mobile Communications Research Laboratory, Frontiers Science Center for Mobile Information Communication and Security, Southeast University, Purple Mountain Laboratories, Nanjing, China; Corresponding authors.Department of Obstetrics and Gynecology Zhongda Hospital, Southeast University, Nanjing, China; Corresponding authors.The incidence rate of human uterine leiomyomas is over 70% in the women of childbearing age, which has caused serious health and financial burden. Our previous study confirmed that Bisphenol A (BPA)，representative environmental estrogen, promoted the proliferation of human uterine leiomyomas and up-regulated the expression of cell proliferation-related genes. In this study, by combining ChIP-seq and RNA-seq, it was shown that after BPA intervention, H3K27ac modification levels and gene expression levels were altered in uterine leiomyomas cells. Moreover experimental verification found that BPA can regulate ITGA2 through the transcription factor XBP1, activate the downstream PI3K/AKT signaling pathway, eventually promote the proliferation of uterine leiomyomas. The present study provides new insights into the pathogenesis associated with exposure to BPA and other endocrine disruptors with similar effects by defining XBP1 as an important regulator, and which may act as an intervention and treatment target for uterine leiomyomas.http://www.sciencedirect.com/science/article/pii/S0147651322010417RNA-seqChIP-seqXBP1Bisphenol AUterine leiomyoma |
| spellingShingle | Zemin Li Han Yin Kai Chen Bo Ding Jingyun Xu Mulan Ren Chuan Zhang Yang Shen Effects of bisphenol A on uterine leiomyoma: In vitro and in vivo evaluation with mechanistic insights related to XBP1 Ecotoxicology and Environmental Safety RNA-seq ChIP-seq XBP1 Bisphenol A Uterine leiomyoma |
| title | Effects of bisphenol A on uterine leiomyoma: In vitro and in vivo evaluation with mechanistic insights related to XBP1 |
| title_full | Effects of bisphenol A on uterine leiomyoma: In vitro and in vivo evaluation with mechanistic insights related to XBP1 |
| title_fullStr | Effects of bisphenol A on uterine leiomyoma: In vitro and in vivo evaluation with mechanistic insights related to XBP1 |
| title_full_unstemmed | Effects of bisphenol A on uterine leiomyoma: In vitro and in vivo evaluation with mechanistic insights related to XBP1 |
| title_short | Effects of bisphenol A on uterine leiomyoma: In vitro and in vivo evaluation with mechanistic insights related to XBP1 |
| title_sort | effects of bisphenol a on uterine leiomyoma in vitro and in vivo evaluation with mechanistic insights related to xbp1 |
| topic | RNA-seq ChIP-seq XBP1 Bisphenol A Uterine leiomyoma |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322010417 |
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