Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes
Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation of the epidermal cells and is clinically presented as thick, bright red to pink plaques with a silvery scale. Photodynamic therapy (PDT) using visible light has become of increasing interest in the treatment of infl...
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/7/1388 |
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| author | Clinton Gomez Chawanphat Muangnoi Feaungthit Niyamissara Sorasitthiyanukarn Jongkonnee Wongpiyabovorn Pornchai Rojsitthisak Pranee Rojsitthisak |
| author_facet | Clinton Gomez Chawanphat Muangnoi Feaungthit Niyamissara Sorasitthiyanukarn Jongkonnee Wongpiyabovorn Pornchai Rojsitthisak Pranee Rojsitthisak |
| author_sort | Clinton Gomez |
| collection | DOAJ |
| description | Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation of the epidermal cells and is clinically presented as thick, bright red to pink plaques with a silvery scale. Photodynamic therapy (PDT) using visible light has become of increasing interest in the treatment of inflammatory skin diseases. In this study, we demonstrate that a combination of curcumin-loaded chitosan/alginate nanoparticles (Cur-CS/Alg NPs) and blue light emitting diodes (LED) light irradiation effectively suppressed the hyperproliferation of tumor necrosis factor-alpha (TNF-α)-induced cultured human kerlatinocyte (HaCaT) cells. The Cur-CS/Alg NPs were fabricated by emulsification of curcumin in aqueous sodium alginate solution and ionotropic gelation with calcium chloride and chitosan using an optimized formulation derived from a Box-Behnken design. The fabricated Cur-CS/Alg NPs were characterized for their particle size, zeta potential, encapsulation efficiency, and loading capacity. The surrogate 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, to measure the relative number of viable cells, showed that the CS/Alg NPs were nontoxic to normal HaCaT cells, while 0.05 µg/mL and 0.1 µg/mL of free curcumin and Cur-CS/Alg NPs inhibited the hyperproliferation of HaCaT cells induced by TNF-α. However, the Cur-CS/Alg NPs demonstrated a stronger effect than the free curcumin, especially when combined with blue light irradiation (10 J/cm<sup>2</sup>) from an LED-based illumination device. Therefore, the Cur-CS/Alg NPs with blue LED light could be potentially developed into an effective PDT system for the treatment of psoriasis. |
| first_indexed | 2024-12-11T15:51:23Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T15:51:23Z |
| publishDate | 2019-04-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d9c824d904b04cc8be36aa5c12f7e49d2022-12-22T00:59:33ZengMDPI AGMolecules1420-30492019-04-01247138810.3390/molecules24071388molecules24071388Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of KeratinocytesClinton Gomez0Chawanphat Muangnoi1Feaungthit Niyamissara Sorasitthiyanukarn2Jongkonnee Wongpiyabovorn3Pornchai Rojsitthisak4Pranee Rojsitthisak5Biomedicinal Chemistry Program, Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, ThailandNatural Products for Ageing and Chronic Diseases Research Unit, Chulalongkorn University, Bangkok 10330, ThailandNatural Products for Ageing and Chronic Diseases Research Unit, Chulalongkorn University, Bangkok 10330, ThailandCenter of Excellence in Immunology and Immune Mediated Diseases, Division of Immunology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandNatural Products for Ageing and Chronic Diseases Research Unit, Chulalongkorn University, Bangkok 10330, ThailandNatural Products for Ageing and Chronic Diseases Research Unit, Chulalongkorn University, Bangkok 10330, ThailandPsoriasis is a chronic inflammatory skin disease characterized by hyperproliferation of the epidermal cells and is clinically presented as thick, bright red to pink plaques with a silvery scale. Photodynamic therapy (PDT) using visible light has become of increasing interest in the treatment of inflammatory skin diseases. In this study, we demonstrate that a combination of curcumin-loaded chitosan/alginate nanoparticles (Cur-CS/Alg NPs) and blue light emitting diodes (LED) light irradiation effectively suppressed the hyperproliferation of tumor necrosis factor-alpha (TNF-α)-induced cultured human kerlatinocyte (HaCaT) cells. The Cur-CS/Alg NPs were fabricated by emulsification of curcumin in aqueous sodium alginate solution and ionotropic gelation with calcium chloride and chitosan using an optimized formulation derived from a Box-Behnken design. The fabricated Cur-CS/Alg NPs were characterized for their particle size, zeta potential, encapsulation efficiency, and loading capacity. The surrogate 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, to measure the relative number of viable cells, showed that the CS/Alg NPs were nontoxic to normal HaCaT cells, while 0.05 µg/mL and 0.1 µg/mL of free curcumin and Cur-CS/Alg NPs inhibited the hyperproliferation of HaCaT cells induced by TNF-α. However, the Cur-CS/Alg NPs demonstrated a stronger effect than the free curcumin, especially when combined with blue light irradiation (10 J/cm<sup>2</sup>) from an LED-based illumination device. Therefore, the Cur-CS/Alg NPs with blue LED light could be potentially developed into an effective PDT system for the treatment of psoriasis.https://www.mdpi.com/1420-3049/24/7/1388photo-irradiationcurcuminchitosan/alginate nanoparticlespsoriasisLED light |
| spellingShingle | Clinton Gomez Chawanphat Muangnoi Feaungthit Niyamissara Sorasitthiyanukarn Jongkonnee Wongpiyabovorn Pornchai Rojsitthisak Pranee Rojsitthisak Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes Molecules photo-irradiation curcumin chitosan/alginate nanoparticles psoriasis LED light |
| title | Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes |
| title_full | Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes |
| title_fullStr | Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes |
| title_full_unstemmed | Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes |
| title_short | Synergistic Effects of Photo-Irradiation and Curcumin-Chitosan/Alginate Nanoparticles on Tumor Necrosis Factor-Alpha-Induced Psoriasis-Like Proliferation of Keratinocytes |
| title_sort | synergistic effects of photo irradiation and curcumin chitosan alginate nanoparticles on tumor necrosis factor alpha induced psoriasis like proliferation of keratinocytes |
| topic | photo-irradiation curcumin chitosan/alginate nanoparticles psoriasis LED light |
| url | https://www.mdpi.com/1420-3049/24/7/1388 |
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