Profusion of G-quadruplexes on both subunits of metazoan ribosomes.
Mammalian and bird ribosomes are nearly twice the mass of prokaryotic ribosomes in part because of their extraordinarily long rRNA tentacles. Human rRNA tentacles are not fully observable in current three-dimensional structures and their conformations remain to be fully resolved. In previous work we...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0226177 |
| _version_ | 1818919750306103296 |
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| author | Santi Mestre-Fos Petar I Penev John Colin Richards William L Dean Robert D Gray Jonathan B Chaires Loren Dean Williams |
| author_facet | Santi Mestre-Fos Petar I Penev John Colin Richards William L Dean Robert D Gray Jonathan B Chaires Loren Dean Williams |
| author_sort | Santi Mestre-Fos |
| collection | DOAJ |
| description | Mammalian and bird ribosomes are nearly twice the mass of prokaryotic ribosomes in part because of their extraordinarily long rRNA tentacles. Human rRNA tentacles are not fully observable in current three-dimensional structures and their conformations remain to be fully resolved. In previous work we identified sequences that favor G-quadruplexes in silico and in vitro in rRNA tentacles of the human large ribosomal subunit. We demonstrated by experiment that these sequences form G-quadruplexes in vitro. Here, using a more recent motif definition, we report additional G-quadruplex sequences on surfaces of both subunits of the human ribosome. The revised sequence definition reveals expansive arrays of potential G-quadruplex sequences on LSU tentacles. In addition, we demonstrate by a variety of experimental methods that fragments of the small subunit rRNA form G-quadruplexes in vitro. Prior to this report rRNA sequences that form G-quadruplexes were confined to the large ribosomal subunit. Our combined results indicate that the surface of the assembled human ribosome contains numerous sequences capable of forming G-quadruplexes on both ribosomal subunits. The data suggest conversion between duplexes and G-quadruplexes in response to association with proteins, ions, or other RNAs. In some systems it seems likely that the integrated population of RNA G-quadruplexes may be dominated by rRNA, which is the most abundant cellular RNA. |
| first_indexed | 2024-12-20T01:10:49Z |
| format | Article |
| id | doaj.art-d9c87c731498483f8c555473ba91cdd0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:10:49Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d9c87c731498483f8c555473ba91cdd02022-12-21T19:58:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011412e022617710.1371/journal.pone.0226177Profusion of G-quadruplexes on both subunits of metazoan ribosomes.Santi Mestre-FosPetar I PenevJohn Colin RichardsWilliam L DeanRobert D GrayJonathan B ChairesLoren Dean WilliamsMammalian and bird ribosomes are nearly twice the mass of prokaryotic ribosomes in part because of their extraordinarily long rRNA tentacles. Human rRNA tentacles are not fully observable in current three-dimensional structures and their conformations remain to be fully resolved. In previous work we identified sequences that favor G-quadruplexes in silico and in vitro in rRNA tentacles of the human large ribosomal subunit. We demonstrated by experiment that these sequences form G-quadruplexes in vitro. Here, using a more recent motif definition, we report additional G-quadruplex sequences on surfaces of both subunits of the human ribosome. The revised sequence definition reveals expansive arrays of potential G-quadruplex sequences on LSU tentacles. In addition, we demonstrate by a variety of experimental methods that fragments of the small subunit rRNA form G-quadruplexes in vitro. Prior to this report rRNA sequences that form G-quadruplexes were confined to the large ribosomal subunit. Our combined results indicate that the surface of the assembled human ribosome contains numerous sequences capable of forming G-quadruplexes on both ribosomal subunits. The data suggest conversion between duplexes and G-quadruplexes in response to association with proteins, ions, or other RNAs. In some systems it seems likely that the integrated population of RNA G-quadruplexes may be dominated by rRNA, which is the most abundant cellular RNA.https://doi.org/10.1371/journal.pone.0226177 |
| spellingShingle | Santi Mestre-Fos Petar I Penev John Colin Richards William L Dean Robert D Gray Jonathan B Chaires Loren Dean Williams Profusion of G-quadruplexes on both subunits of metazoan ribosomes. PLoS ONE |
| title | Profusion of G-quadruplexes on both subunits of metazoan ribosomes. |
| title_full | Profusion of G-quadruplexes on both subunits of metazoan ribosomes. |
| title_fullStr | Profusion of G-quadruplexes on both subunits of metazoan ribosomes. |
| title_full_unstemmed | Profusion of G-quadruplexes on both subunits of metazoan ribosomes. |
| title_short | Profusion of G-quadruplexes on both subunits of metazoan ribosomes. |
| title_sort | profusion of g quadruplexes on both subunits of metazoan ribosomes |
| url | https://doi.org/10.1371/journal.pone.0226177 |
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