Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings.
Acute kidney injury (AKI) is a critical systemic complication caused by Bothrops envenoming, a neglected health problem in the Brazilian Amazon. Understanding the underlying mechanisms leading to AKI is crucial for effectively mitigating the burden of this complication. This study aimed to character...
| Asıl Yazarlar: | , , , , , , , , , , , , , , , , , |
|---|---|
| Materyal Türü: | Makale |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
Public Library of Science (PLoS)
2024-03-01
|
| Seri Bilgileri: | PLoS Neglected Tropical Diseases |
| Online Erişim: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0012072&type=printable |
| _version_ | 1827269943776772096 |
|---|---|
| author | Lisele Maria Brasileiro-Martins Sofia Angiole Cavalcante Thaís Pinto Nascimento Alexandre Vilhena Silva-Neto Marlon Dias Mariano Santos Amanda C Camillo-Andrade Juliana de Saldanha da Gama Fischer Caroline Coelho Ferreira Lucas Barbosa Oliveira Marco Aurelio Sartim Allyson Guimarães Costa Manuela B Pucca Fan Hui Wen Ana Maria Moura-da-Silva Jacqueline Sachett Paulo Costa Carvalho Priscila Ferreira de Aquino Wuelton M Monteiro |
| author_facet | Lisele Maria Brasileiro-Martins Sofia Angiole Cavalcante Thaís Pinto Nascimento Alexandre Vilhena Silva-Neto Marlon Dias Mariano Santos Amanda C Camillo-Andrade Juliana de Saldanha da Gama Fischer Caroline Coelho Ferreira Lucas Barbosa Oliveira Marco Aurelio Sartim Allyson Guimarães Costa Manuela B Pucca Fan Hui Wen Ana Maria Moura-da-Silva Jacqueline Sachett Paulo Costa Carvalho Priscila Ferreira de Aquino Wuelton M Monteiro |
| author_sort | Lisele Maria Brasileiro-Martins |
| collection | DOAJ |
| description | Acute kidney injury (AKI) is a critical systemic complication caused by Bothrops envenoming, a neglected health problem in the Brazilian Amazon. Understanding the underlying mechanisms leading to AKI is crucial for effectively mitigating the burden of this complication. This study aimed to characterize the urinary protein profile of Bothrops atrox snakebite victims who developed AKI. We analyzed three groups of samples collected on admission: healthy subjects (controls, n = 10), snakebite victims who developed AKI (AKI, n = 10), and those who did not evolve to AKI (No-AKI, n = 10). Using liquid-chromatography tandem mass spectrometry, we identified and quantified (label-free) 1190 proteins. A panel of 65 proteins was identified exclusively in the urine of snakebite victims, with 32 exclusives to the AKI condition. Proteins more abundant or exclusive in AKI's urine were associated with acute phase response, endopeptidase inhibition, complement cascade, and inflammation. Notable proteins include serotransferrin, SERPINA-1, alpha-1B-glycoprotein, and NHL repeat-containing protein 3. Furthermore, evaluating previously reported biomarkers candidates for AKI and renal injury, we found retinol-binding protein, beta-2-microglobulin, cystatin-C, and hepcidin to be significant in cases of AKI induced by Bothrops envenoming. This work sheds light on physiological disturbances caused by Bothrops envenoming, highlighting potential biological processes contributing to AKI. Such insights may aid in better understanding and managing this life-threatening complication. |
| first_indexed | 2024-04-24T07:36:46Z |
| format | Article |
| id | doaj.art-d9cb06f2d3d94d6b98de9285e4e92820 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2025-03-22T05:13:34Z |
| publishDate | 2024-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-d9cb06f2d3d94d6b98de9285e4e928202024-04-27T05:33:54ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352024-03-01183e001207210.1371/journal.pntd.0012072Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings.Lisele Maria Brasileiro-MartinsSofia Angiole CavalcanteThaís Pinto NascimentoAlexandre Vilhena Silva-NetoMarlon Dias Mariano SantosAmanda C Camillo-AndradeJuliana de Saldanha da Gama FischerCaroline Coelho FerreiraLucas Barbosa OliveiraMarco Aurelio SartimAllyson Guimarães CostaManuela B PuccaFan Hui WenAna Maria Moura-da-SilvaJacqueline SachettPaulo Costa CarvalhoPriscila Ferreira de AquinoWuelton M MonteiroAcute kidney injury (AKI) is a critical systemic complication caused by Bothrops envenoming, a neglected health problem in the Brazilian Amazon. Understanding the underlying mechanisms leading to AKI is crucial for effectively mitigating the burden of this complication. This study aimed to characterize the urinary protein profile of Bothrops atrox snakebite victims who developed AKI. We analyzed three groups of samples collected on admission: healthy subjects (controls, n = 10), snakebite victims who developed AKI (AKI, n = 10), and those who did not evolve to AKI (No-AKI, n = 10). Using liquid-chromatography tandem mass spectrometry, we identified and quantified (label-free) 1190 proteins. A panel of 65 proteins was identified exclusively in the urine of snakebite victims, with 32 exclusives to the AKI condition. Proteins more abundant or exclusive in AKI's urine were associated with acute phase response, endopeptidase inhibition, complement cascade, and inflammation. Notable proteins include serotransferrin, SERPINA-1, alpha-1B-glycoprotein, and NHL repeat-containing protein 3. Furthermore, evaluating previously reported biomarkers candidates for AKI and renal injury, we found retinol-binding protein, beta-2-microglobulin, cystatin-C, and hepcidin to be significant in cases of AKI induced by Bothrops envenoming. This work sheds light on physiological disturbances caused by Bothrops envenoming, highlighting potential biological processes contributing to AKI. Such insights may aid in better understanding and managing this life-threatening complication.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0012072&type=printable |
| spellingShingle | Lisele Maria Brasileiro-Martins Sofia Angiole Cavalcante Thaís Pinto Nascimento Alexandre Vilhena Silva-Neto Marlon Dias Mariano Santos Amanda C Camillo-Andrade Juliana de Saldanha da Gama Fischer Caroline Coelho Ferreira Lucas Barbosa Oliveira Marco Aurelio Sartim Allyson Guimarães Costa Manuela B Pucca Fan Hui Wen Ana Maria Moura-da-Silva Jacqueline Sachett Paulo Costa Carvalho Priscila Ferreira de Aquino Wuelton M Monteiro Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings. PLoS Neglected Tropical Diseases |
| title | Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings. |
| title_full | Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings. |
| title_fullStr | Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings. |
| title_full_unstemmed | Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings. |
| title_short | Urinary proteomics reveals biological processes related to acute kidney injury in Bothrops atrox envenomings. |
| title_sort | urinary proteomics reveals biological processes related to acute kidney injury in bothrops atrox envenomings |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0012072&type=printable |
| work_keys_str_mv | AT liselemariabrasileiromartins urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT sofiaangiolecavalcante urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT thaispintonascimento urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT alexandrevilhenasilvaneto urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT marlondiasmarianosantos urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT amandaccamilloandrade urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT julianadesaldanhadagamafischer urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT carolinecoelhoferreira urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT lucasbarbosaoliveira urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT marcoaureliosartim urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT allysonguimaraescosta urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT manuelabpucca urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT fanhuiwen urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT anamariamouradasilva urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT jacquelinesachett urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT paulocostacarvalho urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT priscilaferreiradeaquino urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings AT wueltonmmonteiro urinaryproteomicsrevealsbiologicalprocessesrelatedtoacutekidneyinjuryinbothropsatroxenvenomings |