Comparison of Antibacterial Effect of Composite Resins Incorporating Copper with Zinc Oxide Nanoparticles on Streptococcus Mutans
<strong>Introduction:</strong> Polymerization shrinkage causes a gap between the composite resin and the tooth edge which leads to bacterial invasion, secondary caries, recurrent caries, and the final failure of restoration. It seems that the addition of nanoparticles to composites can b...
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| Format: | Article |
| Language: | fas |
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Mashhad University of Medical Sciences
2019-12-01
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| Series: | Journal of Mashhad Dental School |
| Online Access: | http://jmds.mums.ac.ir/article_14273_808ef01d50c2eb1ae2c2aa0a0247abb6.pdf |
| _version_ | 1818509376816676864 |
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| author | Abdolrahim Davari Ahmad Mosaddegh Alireza Daneshkazemi Seyed Mehdi Mortazavi Sanigei |
| author_facet | Abdolrahim Davari Ahmad Mosaddegh Alireza Daneshkazemi Seyed Mehdi Mortazavi Sanigei |
| author_sort | Abdolrahim Davari |
| collection | DOAJ |
| description | <strong>Introduction:</strong> Polymerization shrinkage causes a gap between the composite resin and the tooth edge which leads to bacterial invasion, secondary caries, recurrent caries, and the final failure of restoration. It seems that the addition of nanoparticles to composites can be effective in the reduction of the number and function of microorganisms. The aim of the present study was to compare the antibacterial properties of composite resins containing zinc-oxide with copper-oxide nanoparticles against <em>Streptococcus mutans. </em> <br /><strong>Materials and Methods<em>:</em></strong> A number of 490 discoid tablets containing 0.05%,0.5%, 0.1% nano-copper and nano zinc-oxide particles were prepared from composite resin GC (<em>n</em>=70). Diluted solutions of <em>Streptococcus mutans </em>(ATCC 35668) were prepared and 1 mL of bacterial species was placed on the discs. The discs were transferred to liquid culture media and were incubated at 37℃ for 8 hr. Thereafter, 1 mm of serum was cultured at 37° C for 24 hours on a blood agar containing sheep blood and the total number of bacteria was obtained by colony counting of all bacteria detected on each disk. The data were analyzed in SPSS software (version 17) using Kolmogorov-Smirnov test (k-s), Kruskal-Wallis, and Dunn's test. The significance level was considered as α = 0.5. <br /><strong>Results:</strong> Based on the obtained results, the composites containing 0.1% and 0.5% Nano zinc oxide particles and copper oxide nanoparticles significantly decreased the number of bacteria in 15 and 30 days, as compared to the control group (P-value=0.001). Nonetheless, other groups did not demonstrate any significant difference with the control group (P-value=1). <br /><strong><em>Conclusion:</em></strong> Composite resins containing 0.5% zinc oxide nanoparticles demonstrated the highest antibacterial activity against <em>Streptococcus mutans.</em> On the other hand, both nano-copper and zinc-oxide particles revealed the least antimicrobial activity in the concentration of 0.05%. <br /><strong> </strong> |
| first_indexed | 2024-12-10T22:44:42Z |
| format | Article |
| id | doaj.art-d9cdebe0e6424cc1a2f3436101f8dc3b |
| institution | Directory Open Access Journal |
| issn | 1560-9286 2008-2347 |
| language | fas |
| last_indexed | 2024-12-10T22:44:42Z |
| publishDate | 2019-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Journal of Mashhad Dental School |
| spelling | doaj.art-d9cdebe0e6424cc1a2f3436101f8dc3b2022-12-22T01:30:37ZfasMashhad University of Medical SciencesJournal of Mashhad Dental School1560-92862008-23472019-12-0143434435110.22038/jmds.2019.1427314273Comparison of Antibacterial Effect of Composite Resins Incorporating Copper with Zinc Oxide Nanoparticles on Streptococcus MutansAbdolrahim Davari0Ahmad Mosaddegh1Alireza Daneshkazemi2Seyed Mehdi Mortazavi Sanigei3Professor of Operative and Aesthetic Dentistry, Social Determinant of Oral Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Associate professor of Operative and Aesthetic Dentistry, Social Determinant of Oral Health Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Asistant of microbiology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Dentist, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.<strong>Introduction:</strong> Polymerization shrinkage causes a gap between the composite resin and the tooth edge which leads to bacterial invasion, secondary caries, recurrent caries, and the final failure of restoration. It seems that the addition of nanoparticles to composites can be effective in the reduction of the number and function of microorganisms. The aim of the present study was to compare the antibacterial properties of composite resins containing zinc-oxide with copper-oxide nanoparticles against <em>Streptococcus mutans. </em> <br /><strong>Materials and Methods<em>:</em></strong> A number of 490 discoid tablets containing 0.05%,0.5%, 0.1% nano-copper and nano zinc-oxide particles were prepared from composite resin GC (<em>n</em>=70). Diluted solutions of <em>Streptococcus mutans </em>(ATCC 35668) were prepared and 1 mL of bacterial species was placed on the discs. The discs were transferred to liquid culture media and were incubated at 37℃ for 8 hr. Thereafter, 1 mm of serum was cultured at 37° C for 24 hours on a blood agar containing sheep blood and the total number of bacteria was obtained by colony counting of all bacteria detected on each disk. The data were analyzed in SPSS software (version 17) using Kolmogorov-Smirnov test (k-s), Kruskal-Wallis, and Dunn's test. The significance level was considered as α = 0.5. <br /><strong>Results:</strong> Based on the obtained results, the composites containing 0.1% and 0.5% Nano zinc oxide particles and copper oxide nanoparticles significantly decreased the number of bacteria in 15 and 30 days, as compared to the control group (P-value=0.001). Nonetheless, other groups did not demonstrate any significant difference with the control group (P-value=1). <br /><strong><em>Conclusion:</em></strong> Composite resins containing 0.5% zinc oxide nanoparticles demonstrated the highest antibacterial activity against <em>Streptococcus mutans.</em> On the other hand, both nano-copper and zinc-oxide particles revealed the least antimicrobial activity in the concentration of 0.05%. <br /><strong> </strong>http://jmds.mums.ac.ir/article_14273_808ef01d50c2eb1ae2c2aa0a0247abb6.pdf |
| spellingShingle | Abdolrahim Davari Ahmad Mosaddegh Alireza Daneshkazemi Seyed Mehdi Mortazavi Sanigei Comparison of Antibacterial Effect of Composite Resins Incorporating Copper with Zinc Oxide Nanoparticles on Streptococcus Mutans Journal of Mashhad Dental School |
| title | Comparison of Antibacterial Effect of Composite Resins Incorporating Copper with Zinc Oxide Nanoparticles on Streptococcus Mutans |
| title_full | Comparison of Antibacterial Effect of Composite Resins Incorporating Copper with Zinc Oxide Nanoparticles on Streptococcus Mutans |
| title_fullStr | Comparison of Antibacterial Effect of Composite Resins Incorporating Copper with Zinc Oxide Nanoparticles on Streptococcus Mutans |
| title_full_unstemmed | Comparison of Antibacterial Effect of Composite Resins Incorporating Copper with Zinc Oxide Nanoparticles on Streptococcus Mutans |
| title_short | Comparison of Antibacterial Effect of Composite Resins Incorporating Copper with Zinc Oxide Nanoparticles on Streptococcus Mutans |
| title_sort | comparison of antibacterial effect of composite resins incorporating copper with zinc oxide nanoparticles on streptococcus mutans |
| url | http://jmds.mums.ac.ir/article_14273_808ef01d50c2eb1ae2c2aa0a0247abb6.pdf |
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