Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection
This study is concerned with two sensitive, fast and reproducible approaches; namely, second-derivative synchronous fluorimetry (method I) and reversed phase high-performance liquid chromatography with fluorimetric detection (method II) for synchronized evaluation of losartan (LOS) and amlodipine be...
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| Format: | Article |
| Language: | English |
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The Royal Society
2019-04-01
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| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190310 |
| _version_ | 1819086060324388864 |
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| author | Shereen Shalan Jenny Jeehan Nasr |
| author_facet | Shereen Shalan Jenny Jeehan Nasr |
| author_sort | Shereen Shalan |
| collection | DOAJ |
| description | This study is concerned with two sensitive, fast and reproducible approaches; namely, second-derivative synchronous fluorimetry (method I) and reversed phase high-performance liquid chromatography with fluorimetric detection (method II) for synchronized evaluation of losartan (LOS) and amlodipine besylate (AML). Method I is based on measuring second-derivative synchronous fluorescence spectra of LOS and AML at Δλ = 80 nm in water. The experimental factors influencing the synchronous fluorescence of the considered compounds were sensibly adjusted. The chromatographic analysis was executed on a Nucleodur MN-C18 column of dimensions; 250 × 4.6 mm i.d. and 5 µm particle size). The fluorimetric detection was time-programmed at λem = 440 nm for AML (0.0–7.5 min) and at λem = 400 nm for LOS (7.5–10 min) after excitation at λex = 245 nm. The mobile phase is a blend of acetonitrile with 0.02 M phosphate buffer in a proportion of 45 : 55, pH 4.0, pumped using a flow rate of 1 ml min−1. The calibration plots were established to be 0.1–4.0 µg ml−1 for both drugs in method I and 0.05–4.0 µg ml−1 for both drugs in method II. The study was extended to the evaluation of the two drugs in their co-formulated tablets. |
| first_indexed | 2024-12-21T21:14:15Z |
| format | Article |
| id | doaj.art-d9ceb272ed4d4ce18abd4831c85929af |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-21T21:14:15Z |
| publishDate | 2019-04-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-d9ceb272ed4d4ce18abd4831c85929af2022-12-21T18:50:04ZengThe Royal SocietyRoyal Society Open Science2054-57032019-04-016410.1098/rsos.190310190310Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detectionShereen ShalanJenny Jeehan NasrThis study is concerned with two sensitive, fast and reproducible approaches; namely, second-derivative synchronous fluorimetry (method I) and reversed phase high-performance liquid chromatography with fluorimetric detection (method II) for synchronized evaluation of losartan (LOS) and amlodipine besylate (AML). Method I is based on measuring second-derivative synchronous fluorescence spectra of LOS and AML at Δλ = 80 nm in water. The experimental factors influencing the synchronous fluorescence of the considered compounds were sensibly adjusted. The chromatographic analysis was executed on a Nucleodur MN-C18 column of dimensions; 250 × 4.6 mm i.d. and 5 µm particle size). The fluorimetric detection was time-programmed at λem = 440 nm for AML (0.0–7.5 min) and at λem = 400 nm for LOS (7.5–10 min) after excitation at λex = 245 nm. The mobile phase is a blend of acetonitrile with 0.02 M phosphate buffer in a proportion of 45 : 55, pH 4.0, pumped using a flow rate of 1 ml min−1. The calibration plots were established to be 0.1–4.0 µg ml−1 for both drugs in method I and 0.05–4.0 µg ml−1 for both drugs in method II. The study was extended to the evaluation of the two drugs in their co-formulated tablets.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190310losartanamlodipine besylatesecond-derivative synchronous spectrofluorimetryhigh-performance liquid chromatography and fluorimetric detection |
| spellingShingle | Shereen Shalan Jenny Jeehan Nasr Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection Royal Society Open Science losartan amlodipine besylate second-derivative synchronous spectrofluorimetry high-performance liquid chromatography and fluorimetric detection |
| title | Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection |
| title_full | Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection |
| title_fullStr | Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection |
| title_full_unstemmed | Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection |
| title_short | Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection |
| title_sort | simultaneous evaluation of losartan and amlodipine besylate using second derivative synchronous spectrofluorimetric technique and liquid chromatography with time programmed fluorimetric detection |
| topic | losartan amlodipine besylate second-derivative synchronous spectrofluorimetry high-performance liquid chromatography and fluorimetric detection |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.190310 |
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