YEAR-ROUND BLUEBERRY SCORCH VIRUS DETECTION IN HIGHBUSH BLUEBERRY

Viral diseases are a worldwide problem of blueberry which a major limiting factor for production. A survey for Blueberry scorch virus (BlScV) by DAS-ELISA in various organs of highbush blueberry conducted from May 2010 to April 2011, showed the occurrence of these virus in cvs Bluecrop and Herbert,...

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Main Authors: Elżbieta Paduch-Cichal, Maria Chorodorska, Elżbieta Kalinowska, Beata Komorowska
Format: Article
Language:English
Published: University of Life Sciences in Lublin - Publishing House 2014-06-01
Series:Acta Scientiarum Polonorum: Hortorum Cultus
Subjects:
Online Access:https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2725
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author Elżbieta Paduch-Cichal
Maria Chorodorska
Elżbieta Kalinowska
Beata Komorowska
author_facet Elżbieta Paduch-Cichal
Maria Chorodorska
Elżbieta Kalinowska
Beata Komorowska
author_sort Elżbieta Paduch-Cichal
collection DOAJ
description Viral diseases are a worldwide problem of blueberry which a major limiting factor for production. A survey for Blueberry scorch virus (BlScV) by DAS-ELISA in various organs of highbush blueberry conducted from May 2010 to April 2011, showed the occurrence of these virus in cvs Bluecrop and Herbert, which showing virus-like symptoms. Samples of plant materials (bud flower, flower, leaf, bark) were collected individually from each highbush blueberry plant of every cultivar. It was established that the detection of virus of each the investigated bushes cvs Bluecrop and Herbert depended on the tested plant materials as well as the period in which the tests were performed. The effectiveness of the virus detection varied for the investigated cultivars. The presence of the BlScV was confirmed in leaves samples with specific primer pair which amplifies a 430 bp fragment of the 5’-proximal ORF I [RNA-dependent RNA polymerase (RdRp)].
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spelling doaj.art-d9cfad992ee4420fab7046e2e54280f42023-12-12T09:50:57ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052014-06-01133YEAR-ROUND BLUEBERRY SCORCH VIRUS DETECTION IN HIGHBUSH BLUEBERRYElżbieta Paduch-CichalMaria ChorodorskaElżbieta KalinowskaBeata Komorowska Viral diseases are a worldwide problem of blueberry which a major limiting factor for production. A survey for Blueberry scorch virus (BlScV) by DAS-ELISA in various organs of highbush blueberry conducted from May 2010 to April 2011, showed the occurrence of these virus in cvs Bluecrop and Herbert, which showing virus-like symptoms. Samples of plant materials (bud flower, flower, leaf, bark) were collected individually from each highbush blueberry plant of every cultivar. It was established that the detection of virus of each the investigated bushes cvs Bluecrop and Herbert depended on the tested plant materials as well as the period in which the tests were performed. The effectiveness of the virus detection varied for the investigated cultivars. The presence of the BlScV was confirmed in leaves samples with specific primer pair which amplifies a 430 bp fragment of the 5’-proximal ORF I [RNA-dependent RNA polymerase (RdRp)]. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2725blueberry cultivarsBlScVDAS-ELISART-PCR
spellingShingle Elżbieta Paduch-Cichal
Maria Chorodorska
Elżbieta Kalinowska
Beata Komorowska
YEAR-ROUND BLUEBERRY SCORCH VIRUS DETECTION IN HIGHBUSH BLUEBERRY
Acta Scientiarum Polonorum: Hortorum Cultus
blueberry cultivars
BlScV
DAS-ELISA
RT-PCR
title YEAR-ROUND BLUEBERRY SCORCH VIRUS DETECTION IN HIGHBUSH BLUEBERRY
title_full YEAR-ROUND BLUEBERRY SCORCH VIRUS DETECTION IN HIGHBUSH BLUEBERRY
title_fullStr YEAR-ROUND BLUEBERRY SCORCH VIRUS DETECTION IN HIGHBUSH BLUEBERRY
title_full_unstemmed YEAR-ROUND BLUEBERRY SCORCH VIRUS DETECTION IN HIGHBUSH BLUEBERRY
title_short YEAR-ROUND BLUEBERRY SCORCH VIRUS DETECTION IN HIGHBUSH BLUEBERRY
title_sort year round blueberry scorch virus detection in highbush blueberry
topic blueberry cultivars
BlScV
DAS-ELISA
RT-PCR
url https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2725
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AT mariachorodorska yearroundblueberryscorchvirusdetectioninhighbushblueberry
AT elzbietakalinowska yearroundblueberryscorchvirusdetectioninhighbushblueberry
AT beatakomorowska yearroundblueberryscorchvirusdetectioninhighbushblueberry