A bioinformatics approach to introduce novel multi-epitope vaccines against Acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteins
Increasing antibiotic resistance of Acinetobacter baumannii highlights the urgent need to develop an effective vaccine to combat it. Despite all efforts, no approved vaccine against this bacterium is on the market, and attempts are underway to find effective vaccines. Due to their surface exposure,...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Informatics in Medicine Unlocked |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352914822001320 |
| _version_ | 1811289375482314752 |
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| author | Maryam Meskini Narjes Noori Goodarzi Sepideh Fereshteh Negin Bolourchi Amir Mirzaie Farzad Badmasti |
| author_facet | Maryam Meskini Narjes Noori Goodarzi Sepideh Fereshteh Negin Bolourchi Amir Mirzaie Farzad Badmasti |
| author_sort | Maryam Meskini |
| collection | DOAJ |
| description | Increasing antibiotic resistance of Acinetobacter baumannii highlights the urgent need to develop an effective vaccine to combat it. Despite all efforts, no approved vaccine against this bacterium is on the market, and attempts are underway to find effective vaccines. Due to their surface exposure, outer membrane proteins (OMPs) are excellent vaccine targets. The extracellular loops of OMPs are highly immunogenic. Therefore, in the present study, we aimed to detect the conserved, exposed, and immunogenic linear B-cell epitopes among sixty-five OMPs available in 35 A. baumannii genomes deposited in the Vaxign database. According to defined criteria (allergenicity, antigenicity, and non-similarity to host), four linear B-cell epitopes were selected as highly immunogenic peptides. Epitope shuffling was performed to select the best arrangements of these epitopes. We proposed six different multi-epitope vaccines consisting of two Naked arrangements, two loop-less C-lobe (LCL) structures, and two FliC-based constructs. An LCL-based multi-epitope vaccine showed the best molecular docking score with human TLRs (TLR 1, 2, 4, and 5) and immune simulation results (Th1 cell population, IFN-γ, IL-12, IgM, IgG1, and IgG2 levels). This study provided a valuable library of exposed epitopes including 26 conserved, linear and immunogenic B-cell epitopes (belonged to 14 OMPs), and three optimized multi-epitope vaccines. However, more experimental studies are needed to confirm the actual immunoreactivity of these vaccine candidates. |
| first_indexed | 2024-04-13T03:53:47Z |
| format | Article |
| id | doaj.art-d9d03b2e2147411cb5ba4805243eddfd |
| institution | Directory Open Access Journal |
| issn | 2352-9148 |
| language | English |
| last_indexed | 2024-04-13T03:53:47Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Informatics in Medicine Unlocked |
| spelling | doaj.art-d9d03b2e2147411cb5ba4805243eddfd2022-12-22T03:03:42ZengElsevierInformatics in Medicine Unlocked2352-91482022-01-0131100989A bioinformatics approach to introduce novel multi-epitope vaccines against Acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteinsMaryam Meskini0Narjes Noori Goodarzi1Sepideh Fereshteh2Negin Bolourchi3Amir Mirzaie4Farzad Badmasti5Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran; Mycobacteriology & Pulmonary Research Department, Pasteur Institute of Iran, Tehran, IranDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Bacteriology, Pasteur Institute of Iran, Tehran, IranDepartment of Bacteriology, Pasteur Institute of Iran, Tehran, IranDepartment of Biology, Parand Branch, Islamic Azad University, Parand, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, Iran; Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran; Corresponding author. Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.Increasing antibiotic resistance of Acinetobacter baumannii highlights the urgent need to develop an effective vaccine to combat it. Despite all efforts, no approved vaccine against this bacterium is on the market, and attempts are underway to find effective vaccines. Due to their surface exposure, outer membrane proteins (OMPs) are excellent vaccine targets. The extracellular loops of OMPs are highly immunogenic. Therefore, in the present study, we aimed to detect the conserved, exposed, and immunogenic linear B-cell epitopes among sixty-five OMPs available in 35 A. baumannii genomes deposited in the Vaxign database. According to defined criteria (allergenicity, antigenicity, and non-similarity to host), four linear B-cell epitopes were selected as highly immunogenic peptides. Epitope shuffling was performed to select the best arrangements of these epitopes. We proposed six different multi-epitope vaccines consisting of two Naked arrangements, two loop-less C-lobe (LCL) structures, and two FliC-based constructs. An LCL-based multi-epitope vaccine showed the best molecular docking score with human TLRs (TLR 1, 2, 4, and 5) and immune simulation results (Th1 cell population, IFN-γ, IL-12, IgM, IgG1, and IgG2 levels). This study provided a valuable library of exposed epitopes including 26 conserved, linear and immunogenic B-cell epitopes (belonged to 14 OMPs), and three optimized multi-epitope vaccines. However, more experimental studies are needed to confirm the actual immunoreactivity of these vaccine candidates.http://www.sciencedirect.com/science/article/pii/S2352914822001320Acinetobacter baumanniiLinear B-Cell epitopeEpitope shuffling |
| spellingShingle | Maryam Meskini Narjes Noori Goodarzi Sepideh Fereshteh Negin Bolourchi Amir Mirzaie Farzad Badmasti A bioinformatics approach to introduce novel multi-epitope vaccines against Acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteins Informatics in Medicine Unlocked Acinetobacter baumannii Linear B-Cell epitope Epitope shuffling |
| title | A bioinformatics approach to introduce novel multi-epitope vaccines against Acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteins |
| title_full | A bioinformatics approach to introduce novel multi-epitope vaccines against Acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteins |
| title_fullStr | A bioinformatics approach to introduce novel multi-epitope vaccines against Acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteins |
| title_full_unstemmed | A bioinformatics approach to introduce novel multi-epitope vaccines against Acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteins |
| title_short | A bioinformatics approach to introduce novel multi-epitope vaccines against Acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteins |
| title_sort | bioinformatics approach to introduce novel multi epitope vaccines against acinetobacter baumannii retrieved from immunogenic extracellular loops of outer membrane proteins |
| topic | Acinetobacter baumannii Linear B-Cell epitope Epitope shuffling |
| url | http://www.sciencedirect.com/science/article/pii/S2352914822001320 |
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