Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda)
Abstract Background The invasive and calamitous polyphagous pest Spodoptera frugiperda or commonly known as fall armyworm (FAW) poses serious menace to the global agricultural production. Owing to the revamped invasion of FAW in 2018 in India, present study was undertaken for precise assessment of i...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Biotechnology |
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| Online Access: | https://doi.org/10.1186/s12896-023-00786-6 |
| _version_ | 1797784587054612480 |
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| author | Snigdha Samanta Mritunjoy Barman Himanshu Thakur Swati Chakraborty Gouranga Upadhyaya Deepayan Roy Amitava Banerjee Arunava Samanta Jayanta Tarafdar |
| author_facet | Snigdha Samanta Mritunjoy Barman Himanshu Thakur Swati Chakraborty Gouranga Upadhyaya Deepayan Roy Amitava Banerjee Arunava Samanta Jayanta Tarafdar |
| author_sort | Snigdha Samanta |
| collection | DOAJ |
| description | Abstract Background The invasive and calamitous polyphagous pest Spodoptera frugiperda or commonly known as fall armyworm (FAW) poses serious menace to the global agricultural production. Owing to the revamped invasion of FAW in 2018 in India, present study was undertaken for precise assessment of its genetic identity and pesticide resistance to aid in pest-management strategies. Results To evaluate the diversity in FAW population across Eastern India, mitochondrial COI sequences were used which revealed a low nucleotide diversity. Analysis of molecular variance indicated significant genetic variation between four global geographical FAW populations with lowest differentiation between India and Africa suggesting a present-day and shared origin of FAW. The study demonstrated existence of two different strains (‘R’ strain and ‘C’ strain) based on COI gene marker. However, discrepancies between COI marker and host plant association of FAW was observed. Characterization of Tpi gene revealed abundance of TpiCa1a followed by TpiCa2b and TpiR1a strains respectively. The FAW population showed higher susceptibility towards chlorantraniliprole and spinetoram than cypermethrin. Insecticide resistance genes depicted marked upregulation although with lot of variance. Chlorantraniliprole resistance ratio (RR) exhibited significant correlation with 1950 (Glutathione S-transferase, GST), 9131 (Cytochrome P450, CYP) and 9360 (CYP) genes, while spinetoram and cypermethrin RR was found to correlate with 1950 (GST) and 9360 (CYP) genes. Conclusion This study manifests Indian subcontinent as the potential new hotspot for the growth and distribution of FAW population that can be effectively controlled using chlorantraniliprole and spinetoram. This study also adds novel significant information on FAW population across Eastern India for developing a comprehensive pest management approach for S. frugiperda. |
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| id | doaj.art-d9d42a1c814e4948aa1679113b14e477 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-03-13T00:42:01Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-d9d42a1c814e4948aa1679113b14e4772023-07-09T11:17:28ZengBMCBMC Biotechnology1472-67502023-07-0123111510.1186/s12896-023-00786-6Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda)Snigdha Samanta0Mritunjoy Barman1Himanshu Thakur2Swati Chakraborty3Gouranga Upadhyaya4Deepayan Roy5Amitava Banerjee6Arunava Samanta7Jayanta Tarafdar8Department of Agricultural Entomology, Bidhan Chandra Krishi ViswavidyalayaDepartment of Agricultural Entomology, Bidhan Chandra Krishi ViswavidyalayaDepartment of Entomology, C.S.K. Himachal Pradesh Krishi VishvavidyalayaDepartment of Plant Pathology, Bidhan Chandra Krishi ViswavidyalayaDepartment of Biological Sciences, Indian Institute of Science Education and Research KolkataSchool of Agriculture Science, GD Goenka UniversityDepartment of Agricultural Entomology, Bidhan Chandra Krishi ViswavidyalayaDepartment of Agricultural Entomology, Bidhan Chandra Krishi ViswavidyalayaDepartment of Plant Pathology, Bidhan Chandra Krishi ViswavidyalayaAbstract Background The invasive and calamitous polyphagous pest Spodoptera frugiperda or commonly known as fall armyworm (FAW) poses serious menace to the global agricultural production. Owing to the revamped invasion of FAW in 2018 in India, present study was undertaken for precise assessment of its genetic identity and pesticide resistance to aid in pest-management strategies. Results To evaluate the diversity in FAW population across Eastern India, mitochondrial COI sequences were used which revealed a low nucleotide diversity. Analysis of molecular variance indicated significant genetic variation between four global geographical FAW populations with lowest differentiation between India and Africa suggesting a present-day and shared origin of FAW. The study demonstrated existence of two different strains (‘R’ strain and ‘C’ strain) based on COI gene marker. However, discrepancies between COI marker and host plant association of FAW was observed. Characterization of Tpi gene revealed abundance of TpiCa1a followed by TpiCa2b and TpiR1a strains respectively. The FAW population showed higher susceptibility towards chlorantraniliprole and spinetoram than cypermethrin. Insecticide resistance genes depicted marked upregulation although with lot of variance. Chlorantraniliprole resistance ratio (RR) exhibited significant correlation with 1950 (Glutathione S-transferase, GST), 9131 (Cytochrome P450, CYP) and 9360 (CYP) genes, while spinetoram and cypermethrin RR was found to correlate with 1950 (GST) and 9360 (CYP) genes. Conclusion This study manifests Indian subcontinent as the potential new hotspot for the growth and distribution of FAW population that can be effectively controlled using chlorantraniliprole and spinetoram. This study also adds novel significant information on FAW population across Eastern India for developing a comprehensive pest management approach for S. frugiperda.https://doi.org/10.1186/s12896-023-00786-6Insecticide resistanceCOI markerGenetic diversityFall Armyworm |
| spellingShingle | Snigdha Samanta Mritunjoy Barman Himanshu Thakur Swati Chakraborty Gouranga Upadhyaya Deepayan Roy Amitava Banerjee Arunava Samanta Jayanta Tarafdar Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda) BMC Biotechnology Insecticide resistance COI marker Genetic diversity Fall Armyworm |
| title | Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda) |
| title_full | Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda) |
| title_fullStr | Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda) |
| title_full_unstemmed | Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda) |
| title_short | Evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm (Spodoptera frugiperda) |
| title_sort | evidence of population expansion and insecticide resistance mechanism in invasive fall armyworm spodoptera frugiperda |
| topic | Insecticide resistance COI marker Genetic diversity Fall Armyworm |
| url | https://doi.org/10.1186/s12896-023-00786-6 |
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