A Comparison of Methods for Identifying Enterobacterales Isolates from Fish and Prawns
Enterobacterales is a prevalent order, which inhabits a variety of environments including food. Due to the high similarities between pathogenic and non-pathogenic species, their identification might be difficult and laborious, and therefore there is a need for rapid and precise identification. The a...
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MDPI AG
2022-03-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/4/410 |
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| author | Arkadiusz Józef Zakrzewski Urszula Zarzecka Wioleta Chajęcka-Wierzchowska Anna Zadernowska |
| author_facet | Arkadiusz Józef Zakrzewski Urszula Zarzecka Wioleta Chajęcka-Wierzchowska Anna Zadernowska |
| author_sort | Arkadiusz Józef Zakrzewski |
| collection | DOAJ |
| description | Enterobacterales is a prevalent order, which inhabits a variety of environments including food. Due to the high similarities between pathogenic and non-pathogenic species, their identification might be difficult and laborious, and therefore there is a need for rapid and precise identification. The aim of this study was to compare the effectiveness of the available methods of identifying order <i>Enterobacterales</i> strains isolated from fresh fish and shrimps (n = 62). The following methods were used in this study: biochemical, sequencing and identification using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). For this purpose, biochemical identification was performed with the use of the EnteroTest 24N set, while the identification using the MALDI-TOF MS technology was operated on VITEK<sup>®</sup> MS. Results were compared with identification made by 16S rRNA sequencing. The results of the study showed that conventional identification methods might provide a false result. Identification by VITEK<sup>®</sup> MS to the species level was correct at 70.97%, and the accuracy of EnteroTest 24N identification did not exceed 50.0%. The genus identification reached 90.32% for the MALDI-TOF technique, while for EnteroTest 24N it was nearly 70.0%. Due to errors in identification, especially of pathogenic organisms, the use of each of these methods should be confirmed by another method, preferably sequencing. |
| first_indexed | 2024-03-09T10:31:33Z |
| format | Article |
| id | doaj.art-d9d63babeae44d1d8a481cb282d43fd6 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T10:31:33Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-d9d63babeae44d1d8a481cb282d43fd62023-12-01T21:17:41ZengMDPI AGPathogens2076-08172022-03-0111441010.3390/pathogens11040410A Comparison of Methods for Identifying Enterobacterales Isolates from Fish and PrawnsArkadiusz Józef Zakrzewski0Urszula Zarzecka1Wioleta Chajęcka-Wierzchowska2Anna Zadernowska3Department of Industrial and Food Microbiology, University of Warmia and Mazury, Plac Cieszyński 1, 10-726 Olsztyn, PolandDepartment of Industrial and Food Microbiology, University of Warmia and Mazury, Plac Cieszyński 1, 10-726 Olsztyn, PolandDepartment of Industrial and Food Microbiology, University of Warmia and Mazury, Plac Cieszyński 1, 10-726 Olsztyn, PolandDepartment of Industrial and Food Microbiology, University of Warmia and Mazury, Plac Cieszyński 1, 10-726 Olsztyn, PolandEnterobacterales is a prevalent order, which inhabits a variety of environments including food. Due to the high similarities between pathogenic and non-pathogenic species, their identification might be difficult and laborious, and therefore there is a need for rapid and precise identification. The aim of this study was to compare the effectiveness of the available methods of identifying order <i>Enterobacterales</i> strains isolated from fresh fish and shrimps (n = 62). The following methods were used in this study: biochemical, sequencing and identification using the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). For this purpose, biochemical identification was performed with the use of the EnteroTest 24N set, while the identification using the MALDI-TOF MS technology was operated on VITEK<sup>®</sup> MS. Results were compared with identification made by 16S rRNA sequencing. The results of the study showed that conventional identification methods might provide a false result. Identification by VITEK<sup>®</sup> MS to the species level was correct at 70.97%, and the accuracy of EnteroTest 24N identification did not exceed 50.0%. The genus identification reached 90.32% for the MALDI-TOF technique, while for EnteroTest 24N it was nearly 70.0%. Due to errors in identification, especially of pathogenic organisms, the use of each of these methods should be confirmed by another method, preferably sequencing.https://www.mdpi.com/2076-0817/11/4/410<i>16S rRNA</i> sequencingEnterobacteralesEnteropathogensEnteroTest 24NMALDI-TOF MS |
| spellingShingle | Arkadiusz Józef Zakrzewski Urszula Zarzecka Wioleta Chajęcka-Wierzchowska Anna Zadernowska A Comparison of Methods for Identifying Enterobacterales Isolates from Fish and Prawns Pathogens <i>16S rRNA</i> sequencing Enterobacterales Enteropathogens EnteroTest 24N MALDI-TOF MS |
| title | A Comparison of Methods for Identifying Enterobacterales Isolates from Fish and Prawns |
| title_full | A Comparison of Methods for Identifying Enterobacterales Isolates from Fish and Prawns |
| title_fullStr | A Comparison of Methods for Identifying Enterobacterales Isolates from Fish and Prawns |
| title_full_unstemmed | A Comparison of Methods for Identifying Enterobacterales Isolates from Fish and Prawns |
| title_short | A Comparison of Methods for Identifying Enterobacterales Isolates from Fish and Prawns |
| title_sort | comparison of methods for identifying enterobacterales isolates from fish and prawns |
| topic | <i>16S rRNA</i> sequencing Enterobacterales Enteropathogens EnteroTest 24N MALDI-TOF MS |
| url | https://www.mdpi.com/2076-0817/11/4/410 |
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