Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.
Generation of potent anti-HIV antibody responses in mucosal compartments is a potential requirement of a transmission-blocking HIV vaccine. HIV-specific, functional antibody responses are present in breast milk, and these mucosal antibody responses may play a role in protection of the majority of HI...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3356285?pdf=render |
| _version_ | 1828396861937942528 |
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| author | James Friedman S Munir Alam Xiaoying Shen Shi-Mao Xia Shelley Stewart Kara Anasti Justin Pollara Genevieve G Fouda Guang Yang Garnett Kelsoe Guido Ferrari Georgia D Tomaras Barton F Haynes Hua-Xin Liao M Anthony Moody Sallie R Permar |
| author_facet | James Friedman S Munir Alam Xiaoying Shen Shi-Mao Xia Shelley Stewart Kara Anasti Justin Pollara Genevieve G Fouda Guang Yang Garnett Kelsoe Guido Ferrari Georgia D Tomaras Barton F Haynes Hua-Xin Liao M Anthony Moody Sallie R Permar |
| author_sort | James Friedman |
| collection | DOAJ |
| description | Generation of potent anti-HIV antibody responses in mucosal compartments is a potential requirement of a transmission-blocking HIV vaccine. HIV-specific, functional antibody responses are present in breast milk, and these mucosal antibody responses may play a role in protection of the majority of HIV-exposed, breastfeeding infants. Therefore, characterization of HIV-specific antibodies produced by B cells in milk could guide the development of vaccines that elicit protective mucosal antibody responses.We isolated B cells from colostrum of an HIV-infected lactating woman with a detectable neutralization response in milk and recombinantly produced and characterized the resulting HIV-1 Envelope (Env)-specific monoclonal antibodies (mAbs).The identified HIV-1 Env-specific colostrum mAbs, CH07 and CH08, represent two of the first mucosally-derived anti-HIV antibodies yet to be reported. Colostrum mAb CH07 is a highly-autoreactive, weakly-neutralizing gp140-specific mAb that binds to linear epitopes in the gp120 C5 region and gp41 fusion domain. In contrast, colostrum mAb CH08 is a nonpolyreactive CD4-inducible (CD4i) gp120-specific mAb with moderate breadth of neutralization.These novel HIV-neutralizing mAbs isolated from a mucosal compartment provide insight into the ability of mucosal B cell populations to produce functional anti-HIV antibodies that may contribute to protection against virus acquisition at mucosal surfaces. |
| first_indexed | 2024-12-10T08:37:29Z |
| format | Article |
| id | doaj.art-d9d8084dda2f4707b5a235ec76f608df |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T08:37:29Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d9d8084dda2f4707b5a235ec76f608df2022-12-22T01:55:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3764810.1371/journal.pone.0037648Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum.James FriedmanS Munir AlamXiaoying ShenShi-Mao XiaShelley StewartKara AnastiJustin PollaraGenevieve G FoudaGuang YangGarnett KelsoeGuido FerrariGeorgia D TomarasBarton F HaynesHua-Xin LiaoM Anthony MoodySallie R PermarGeneration of potent anti-HIV antibody responses in mucosal compartments is a potential requirement of a transmission-blocking HIV vaccine. HIV-specific, functional antibody responses are present in breast milk, and these mucosal antibody responses may play a role in protection of the majority of HIV-exposed, breastfeeding infants. Therefore, characterization of HIV-specific antibodies produced by B cells in milk could guide the development of vaccines that elicit protective mucosal antibody responses.We isolated B cells from colostrum of an HIV-infected lactating woman with a detectable neutralization response in milk and recombinantly produced and characterized the resulting HIV-1 Envelope (Env)-specific monoclonal antibodies (mAbs).The identified HIV-1 Env-specific colostrum mAbs, CH07 and CH08, represent two of the first mucosally-derived anti-HIV antibodies yet to be reported. Colostrum mAb CH07 is a highly-autoreactive, weakly-neutralizing gp140-specific mAb that binds to linear epitopes in the gp120 C5 region and gp41 fusion domain. In contrast, colostrum mAb CH08 is a nonpolyreactive CD4-inducible (CD4i) gp120-specific mAb with moderate breadth of neutralization.These novel HIV-neutralizing mAbs isolated from a mucosal compartment provide insight into the ability of mucosal B cell populations to produce functional anti-HIV antibodies that may contribute to protection against virus acquisition at mucosal surfaces.http://europepmc.org/articles/PMC3356285?pdf=render |
| spellingShingle | James Friedman S Munir Alam Xiaoying Shen Shi-Mao Xia Shelley Stewart Kara Anasti Justin Pollara Genevieve G Fouda Guang Yang Garnett Kelsoe Guido Ferrari Georgia D Tomaras Barton F Haynes Hua-Xin Liao M Anthony Moody Sallie R Permar Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. PLoS ONE |
| title | Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. |
| title_full | Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. |
| title_fullStr | Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. |
| title_full_unstemmed | Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. |
| title_short | Isolation of HIV-1-neutralizing mucosal monoclonal antibodies from human colostrum. |
| title_sort | isolation of hiv 1 neutralizing mucosal monoclonal antibodies from human colostrum |
| url | http://europepmc.org/articles/PMC3356285?pdf=render |
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