Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS)
Microbial tolerance to toxic compounds formed during biomass pretreatment is a significant challenge to produce bio-based products from lignocellulose cost effectively. Rational engineering can be problematic due to insufficient prerequisite knowledge of tolerance mechanisms. Therefore, adaptive lab...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Metabolic Engineering Communications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214030123000068 |
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| author | Jasper L.S.P. Driessen Josefin Johnsen Ivan Pogrebnyakov Elsayed T.T. Mohamed Solange I. Mussatto Adam M. Feist Sheila I. Jensen Alex T. Nielsen |
| author_facet | Jasper L.S.P. Driessen Josefin Johnsen Ivan Pogrebnyakov Elsayed T.T. Mohamed Solange I. Mussatto Adam M. Feist Sheila I. Jensen Alex T. Nielsen |
| author_sort | Jasper L.S.P. Driessen |
| collection | DOAJ |
| description | Microbial tolerance to toxic compounds formed during biomass pretreatment is a significant challenge to produce bio-based products from lignocellulose cost effectively. Rational engineering can be problematic due to insufficient prerequisite knowledge of tolerance mechanisms. Therefore, adaptive laboratory evolution was applied to obtain 20 tolerant lineages of Bacillus subtilis strains able to utilize Distiller's Dried Grains with Solubles-derived (DDGS) hydrolysate. Evolved strains showed both improved growth performance and retained heterologous enzyme production using 100% hydrolysate-based medium, whereas growth of the starting strains was essentially absent. Whole-genome resequencing revealed that evolved isolates acquired mutations in the global regulator codY in 15 of the 19 sequenced isolates. Furthermore, mutations in genes related to oxidative stress (katA, perR) and flagella function appeared in both tolerance and control evolution experiments without toxic compounds. Overall, tolerance adaptive laboratory evolution yielded strains able to utilize DDGS-hydrolysate to produce enzymes and hence proved to be a valuable tool for the valorization of lignocellulose. |
| first_indexed | 2024-03-13T04:44:53Z |
| format | Article |
| id | doaj.art-d9dacf8e27544532aac2078f90d669d9 |
| institution | Directory Open Access Journal |
| issn | 2214-0301 |
| language | English |
| last_indexed | 2024-03-13T04:44:53Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolic Engineering Communications |
| spelling | doaj.art-d9dacf8e27544532aac2078f90d669d92023-06-19T04:28:11ZengElsevierMetabolic Engineering Communications2214-03012023-06-0116e00223Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS)Jasper L.S.P. Driessen0Josefin Johnsen1Ivan Pogrebnyakov2Elsayed T.T. Mohamed3Solange I. Mussatto4Adam M. Feist5Sheila I. Jensen6Alex T. Nielsen7Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220, 2800, Kongens Lyngby, DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220, 2800, Kongens Lyngby, DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220, 2800, Kongens Lyngby, DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220, 2800, Kongens Lyngby, DenmarkDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 223, 2800, Kongens Lyngby, DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220, 2800, Kongens Lyngby, Denmark; Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0412, USANovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220, 2800, Kongens Lyngby, Denmark; Corresponding author.Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 220, 2800, Kongens Lyngby, DenmarkMicrobial tolerance to toxic compounds formed during biomass pretreatment is a significant challenge to produce bio-based products from lignocellulose cost effectively. Rational engineering can be problematic due to insufficient prerequisite knowledge of tolerance mechanisms. Therefore, adaptive laboratory evolution was applied to obtain 20 tolerant lineages of Bacillus subtilis strains able to utilize Distiller's Dried Grains with Solubles-derived (DDGS) hydrolysate. Evolved strains showed both improved growth performance and retained heterologous enzyme production using 100% hydrolysate-based medium, whereas growth of the starting strains was essentially absent. Whole-genome resequencing revealed that evolved isolates acquired mutations in the global regulator codY in 15 of the 19 sequenced isolates. Furthermore, mutations in genes related to oxidative stress (katA, perR) and flagella function appeared in both tolerance and control evolution experiments without toxic compounds. Overall, tolerance adaptive laboratory evolution yielded strains able to utilize DDGS-hydrolysate to produce enzymes and hence proved to be a valuable tool for the valorization of lignocellulose.http://www.sciencedirect.com/science/article/pii/S2214030123000068Adaptive laboratory evolutionLignocelluloseEnzyme productionToleranceBacillus subtilis |
| spellingShingle | Jasper L.S.P. Driessen Josefin Johnsen Ivan Pogrebnyakov Elsayed T.T. Mohamed Solange I. Mussatto Adam M. Feist Sheila I. Jensen Alex T. Nielsen Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS) Metabolic Engineering Communications Adaptive laboratory evolution Lignocellulose Enzyme production Tolerance Bacillus subtilis |
| title | Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS) |
| title_full | Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS) |
| title_fullStr | Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS) |
| title_full_unstemmed | Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS) |
| title_short | Adaptive laboratory evolution of Bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from Distiller's dried grains with solubles (DDGS) |
| title_sort | adaptive laboratory evolution of bacillus subtilis to overcome toxicity of lignocellulosic hydrolysate derived from distiller s dried grains with solubles ddgs |
| topic | Adaptive laboratory evolution Lignocellulose Enzyme production Tolerance Bacillus subtilis |
| url | http://www.sciencedirect.com/science/article/pii/S2214030123000068 |
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