Expression and purification of soluble and active human enterokinase light chain in Escherichia coli

Expression and purification of soluble and active human enterokinase light chain in Escherichia coli

Human enterokinase light chain (hEKL) specifically cleaves the sequence (Asp)4-Lys↓X (D4K), making this a frequently used enzyme for site-specific cleavage of recombinant fusion proteins. However, hEKL production from Escherichia coli is limited due to intramolecular disulphide bonds. Here, we prese...

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Bibliographic Details
Main Authors: Young Su Kim, Hye-Jeong Lee, Sang-hyun Park, Yeu-chun Kim, Jungoh Ahn
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:Biotechnology Reports
Subjects:
Human enterokinase light chain
Escherichia coli
Recombinant protein
Fusion technology
Self-cleavage
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X21000424

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http://www.sciencedirect.com/science/article/pii/S2215017X21000424

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