Antiangiogenic, Anti-Inflammatory and Antioxidant Properties of <i>Bidens tripartite</i> Herb, <i>Galium verum</i> Herb and <i>Rumex hydrolapathum</i> Root
Plants are commonly used in folk medicine. Research indicates that the mechanisms of biological activity of plant extracts may be essential in the treatment of various diseases. In this respect, we decided to test the ethanolic extracts of <i>Bidens tripartita</i> herb (BTH), <i>Ga...
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MDPI AG
2023-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/13/4966 |
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| author | Katarzyna Antoniak Elżbieta Studzińska-Sroka Marcin Szymański Marlena Dudek-Makuch Judyta Cielecka-Piontek Katarzyna Korybalska |
| author_facet | Katarzyna Antoniak Elżbieta Studzińska-Sroka Marcin Szymański Marlena Dudek-Makuch Judyta Cielecka-Piontek Katarzyna Korybalska |
| author_sort | Katarzyna Antoniak |
| collection | DOAJ |
| description | Plants are commonly used in folk medicine. Research indicates that the mechanisms of biological activity of plant extracts may be essential in the treatment of various diseases. In this respect, we decided to test the ethanolic extracts of <i>Bidens tripartita</i> herb (BTH), <i>Galium verum</i> herb (GVH), and <i>Rumicis hydrolapathum</i> root (RHR) on angiogenic, anti-inflammatory, and antioxidant properties and their total polyphenols content. In vitro studies using endothelial cells were used to see tested extracts’ angiogenic/angiostatic and anti-inflammatory properties. The DPPH assay and FRAP analysis were used to detect antioxidant properties of extracts. The Folin-Ciocalteu analysis was used to determine the content of total polyphenols. The results of gas chromatography–mass spectrometry analysis was also presented. In vitro study demonstrated that BTH, GVH, and RHR ethanolic extracts significantly increased cell invasiveness, compared with the control group. Increased endothelial proangiogenic invasiveness was accompanied by reduced metalloproteinase inhibitor 1 (TIMP-1) and raised in metalloproteinase 9 (MMP-9). Only BTH and GVH significantly reduced cell proliferation, while BTH and RHR facilitated migration. Additionally, tested extracts reduced the production of proangiogenic platelet-derived growth factor (PDGF) and hepatocyte growth factor (HGF). The most potent anti-inflammatory capacity showed BTH and GVH, reducing proinflammatory interleukin 8 (CXCL8) and interleukin 6 (Il-6), compared to RHR extract that has slightly less inhibited CXCL8 production without affecting IL-6 production. Moreover, we confirmed the antioxidant properties of all examined extracts. The highest activity was characterized by RHR, which has been correlated with the high content of polyphenols. In conclusion, the modifying influence of examined extracts can be promising in disorders with pathogenesis related to angiogenesis, inflammation and free radicals formation. BTH is the best choice among the three tested extracts with its antiangiogenic and anti-inflammatory properties. |
| first_indexed | 2024-03-11T01:34:37Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T01:34:37Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-d9de6318dcf94731840795de589d062a2023-11-18T17:06:12ZengMDPI AGMolecules1420-30492023-06-012813496610.3390/molecules28134966Antiangiogenic, Anti-Inflammatory and Antioxidant Properties of <i>Bidens tripartite</i> Herb, <i>Galium verum</i> Herb and <i>Rumex hydrolapathum</i> RootKatarzyna Antoniak0Elżbieta Studzińska-Sroka1Marcin Szymański2Marlena Dudek-Makuch3Judyta Cielecka-Piontek4Katarzyna Korybalska5Independent Researcher, 92-507 Łódź, PolandDeparatment of Pharmacognosy and Biomaterials, Poznan University of Medical Science, Rokietnicka 3 Str., 60-806 Poznań, PolandCentre for Advanced Technologies, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 10 Str., 61-614 Poznań, PolandRegulatory Affairs Department, Curtis Health Caps S.A., Batorowska 52 Str., Wysogotowo, 62-081 Przeźmierowo, PolandDeparatment of Pharmacognosy and Biomaterials, Poznan University of Medical Science, Rokietnicka 3 Str., 60-806 Poznań, PolandDepartment of Patophysiology, Poznań University of Medical Science, Rokietnicka 8 Str., 60-806 Poznań, PolandPlants are commonly used in folk medicine. Research indicates that the mechanisms of biological activity of plant extracts may be essential in the treatment of various diseases. In this respect, we decided to test the ethanolic extracts of <i>Bidens tripartita</i> herb (BTH), <i>Galium verum</i> herb (GVH), and <i>Rumicis hydrolapathum</i> root (RHR) on angiogenic, anti-inflammatory, and antioxidant properties and their total polyphenols content. In vitro studies using endothelial cells were used to see tested extracts’ angiogenic/angiostatic and anti-inflammatory properties. The DPPH assay and FRAP analysis were used to detect antioxidant properties of extracts. The Folin-Ciocalteu analysis was used to determine the content of total polyphenols. The results of gas chromatography–mass spectrometry analysis was also presented. In vitro study demonstrated that BTH, GVH, and RHR ethanolic extracts significantly increased cell invasiveness, compared with the control group. Increased endothelial proangiogenic invasiveness was accompanied by reduced metalloproteinase inhibitor 1 (TIMP-1) and raised in metalloproteinase 9 (MMP-9). Only BTH and GVH significantly reduced cell proliferation, while BTH and RHR facilitated migration. Additionally, tested extracts reduced the production of proangiogenic platelet-derived growth factor (PDGF) and hepatocyte growth factor (HGF). The most potent anti-inflammatory capacity showed BTH and GVH, reducing proinflammatory interleukin 8 (CXCL8) and interleukin 6 (Il-6), compared to RHR extract that has slightly less inhibited CXCL8 production without affecting IL-6 production. Moreover, we confirmed the antioxidant properties of all examined extracts. The highest activity was characterized by RHR, which has been correlated with the high content of polyphenols. In conclusion, the modifying influence of examined extracts can be promising in disorders with pathogenesis related to angiogenesis, inflammation and free radicals formation. BTH is the best choice among the three tested extracts with its antiangiogenic and anti-inflammatory properties.https://www.mdpi.com/1420-3049/28/13/4966polyphenolsfree radicalsinvasivenessmigrationproliferation |
| spellingShingle | Katarzyna Antoniak Elżbieta Studzińska-Sroka Marcin Szymański Marlena Dudek-Makuch Judyta Cielecka-Piontek Katarzyna Korybalska Antiangiogenic, Anti-Inflammatory and Antioxidant Properties of <i>Bidens tripartite</i> Herb, <i>Galium verum</i> Herb and <i>Rumex hydrolapathum</i> Root Molecules polyphenols free radicals invasiveness migration proliferation |
| title | Antiangiogenic, Anti-Inflammatory and Antioxidant Properties of <i>Bidens tripartite</i> Herb, <i>Galium verum</i> Herb and <i>Rumex hydrolapathum</i> Root |
| title_full | Antiangiogenic, Anti-Inflammatory and Antioxidant Properties of <i>Bidens tripartite</i> Herb, <i>Galium verum</i> Herb and <i>Rumex hydrolapathum</i> Root |
| title_fullStr | Antiangiogenic, Anti-Inflammatory and Antioxidant Properties of <i>Bidens tripartite</i> Herb, <i>Galium verum</i> Herb and <i>Rumex hydrolapathum</i> Root |
| title_full_unstemmed | Antiangiogenic, Anti-Inflammatory and Antioxidant Properties of <i>Bidens tripartite</i> Herb, <i>Galium verum</i> Herb and <i>Rumex hydrolapathum</i> Root |
| title_short | Antiangiogenic, Anti-Inflammatory and Antioxidant Properties of <i>Bidens tripartite</i> Herb, <i>Galium verum</i> Herb and <i>Rumex hydrolapathum</i> Root |
| title_sort | antiangiogenic anti inflammatory and antioxidant properties of i bidens tripartite i herb i galium verum i herb and i rumex hydrolapathum i root |
| topic | polyphenols free radicals invasiveness migration proliferation |
| url | https://www.mdpi.com/1420-3049/28/13/4966 |
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