HGF/c-Met signaling regulates early differentiation of placental trophoblast cells
Depletion of hepatocyte growth factor (HGF) or mesenchymal-epithelial transition factor (c-Met) in mice leads to fetal lethality and placental maldevelopment. However, the dynamic change pattern of HGF/c-Met signaling during placental development and its involvement in the early differentiation of t...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Society for Reproduction and Development
2021-01-01
|
| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/67/2/67_2020-107/_pdf/-char/en |
| _version_ | 1797615617097859072 |
|---|---|
| author | Yeling MA Xin YU Yu-xia LI Yan-Ling WANG |
| author_facet | Yeling MA Xin YU Yu-xia LI Yan-Ling WANG |
| author_sort | Yeling MA |
| collection | DOAJ |
| description | Depletion of hepatocyte growth factor (HGF) or mesenchymal-epithelial transition factor (c-Met) in mice leads to fetal lethality and placental maldevelopment. However, the dynamic change pattern of HGF/c-Met signaling during placental development and its involvement in the early differentiation of trophoblasts remain to be elucidated. In this study, using in situ hybridization assay, we elaborately demonstrated the spatial-temporal expression of Hgf and c-Met in mouse placenta from E5.5, the very early stage after embryonic implantation, to E12.5, when the placental structure is well developed. The concentration of the soluble form of c-Met (sMet) in maternal circulation peaked at E10.5. By utilizing the induced differentiation model of mouse trophoblast stem cells (mTSCs), we found that HGF significantly promoted mTSC differentiation into syncytiotrophoblasts (STBs) and invasive parietal trophoblast giant cells (PTGCs). Interestingly, sMet efficiently reversed the effect of HGF on mTSC differentiation. These findings indicate that HGF/c-Met signaling participates in regulating placental trophoblast cell fate at the early differentiation stage and that sMet acts as an endogenous antagonist in this aspect. |
| first_indexed | 2024-03-11T07:29:13Z |
| format | Article |
| id | doaj.art-d9e188b6cdf740aaad356e5f058a50e5 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:29:13Z |
| publishDate | 2021-01-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-d9e188b6cdf740aaad356e5f058a50e52023-11-17T07:27:41ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002021-01-01672899710.1262/jrd.2020-107jrdHGF/c-Met signaling regulates early differentiation of placental trophoblast cellsYeling MA0Xin YU1Yu-xia LI2Yan-Ling WANG3State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaDepletion of hepatocyte growth factor (HGF) or mesenchymal-epithelial transition factor (c-Met) in mice leads to fetal lethality and placental maldevelopment. However, the dynamic change pattern of HGF/c-Met signaling during placental development and its involvement in the early differentiation of trophoblasts remain to be elucidated. In this study, using in situ hybridization assay, we elaborately demonstrated the spatial-temporal expression of Hgf and c-Met in mouse placenta from E5.5, the very early stage after embryonic implantation, to E12.5, when the placental structure is well developed. The concentration of the soluble form of c-Met (sMet) in maternal circulation peaked at E10.5. By utilizing the induced differentiation model of mouse trophoblast stem cells (mTSCs), we found that HGF significantly promoted mTSC differentiation into syncytiotrophoblasts (STBs) and invasive parietal trophoblast giant cells (PTGCs). Interestingly, sMet efficiently reversed the effect of HGF on mTSC differentiation. These findings indicate that HGF/c-Met signaling participates in regulating placental trophoblast cell fate at the early differentiation stage and that sMet acts as an endogenous antagonist in this aspect.https://www.jstage.jst.go.jp/article/jrd/67/2/67_2020-107/_pdf/-char/enhepatocyte growth factor (hgf)mesenchymal-epithelial transition factor (c-met)placentasoluble form of c-met (smet)syncytiotrophoblast cellstrophoblast giant cells |
| spellingShingle | Yeling MA Xin YU Yu-xia LI Yan-Ling WANG HGF/c-Met signaling regulates early differentiation of placental trophoblast cells The Journal of Reproduction and Development hepatocyte growth factor (hgf) mesenchymal-epithelial transition factor (c-met) placenta soluble form of c-met (smet) syncytiotrophoblast cells trophoblast giant cells |
| title | HGF/c-Met signaling regulates early differentiation of placental trophoblast cells |
| title_full | HGF/c-Met signaling regulates early differentiation of placental trophoblast cells |
| title_fullStr | HGF/c-Met signaling regulates early differentiation of placental trophoblast cells |
| title_full_unstemmed | HGF/c-Met signaling regulates early differentiation of placental trophoblast cells |
| title_short | HGF/c-Met signaling regulates early differentiation of placental trophoblast cells |
| title_sort | hgf c met signaling regulates early differentiation of placental trophoblast cells |
| topic | hepatocyte growth factor (hgf) mesenchymal-epithelial transition factor (c-met) placenta soluble form of c-met (smet) syncytiotrophoblast cells trophoblast giant cells |
| url | https://www.jstage.jst.go.jp/article/jrd/67/2/67_2020-107/_pdf/-char/en |
| work_keys_str_mv | AT yelingma hgfcmetsignalingregulatesearlydifferentiationofplacentaltrophoblastcells AT xinyu hgfcmetsignalingregulatesearlydifferentiationofplacentaltrophoblastcells AT yuxiali hgfcmetsignalingregulatesearlydifferentiationofplacentaltrophoblastcells AT yanlingwang hgfcmetsignalingregulatesearlydifferentiationofplacentaltrophoblastcells |