Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture
The supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed of a combination of Gelucire<sup>®</sup> 50/13 and Precirol<sup>®</sup> ATO5 to administer...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/2/344 |
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| author | Adriana Trapani María Ángeles Esteban Francesca Curci Daniela Erminia Manno Antonio Serra Giuseppe Fracchiolla Cristóbal Espinosa-Ruiz Stefano Castellani Massimo Conese |
| author_facet | Adriana Trapani María Ángeles Esteban Francesca Curci Daniela Erminia Manno Antonio Serra Giuseppe Fracchiolla Cristóbal Espinosa-Ruiz Stefano Castellani Massimo Conese |
| author_sort | Adriana Trapani |
| collection | DOAJ |
| description | The supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed of a combination of Gelucire<sup>®</sup> 50/13 and Precirol<sup>®</sup> ATO5 to administer a grape seed extract (GSE) mixture containing several antioxidant compounds. The combination of the two lipids for the SLN formation resulted in colloids exhibiting mean particle sizes in the range 139–283 nm and zeta potential values in the range +25.6–43.4 mV. Raman spectra and X-ray diffraction evidenced structural differences between the free GSE and GSE-loaded SLN, leading to the conclusion that GSE alters the structure of the lipid nanocarriers. From a biological viewpoint, cell lines from gilthead seabream and European sea bass were exposed to different concentrations of GSE-SLN for 24 h. In general, at appropriate concentrations, GSE-SLN increased the viability of the fish cells. Furthermore, regarding the gene expression in those cells, the expression of antioxidant genes was upregulated, whereas the expression of <i>hsp70</i> and other genes related to the cytoskeleton was downregulated. Hence, an SLN formulation containing Gelucire<sup>®</sup> 50/13/Precirol<sup>®</sup> ATO5 and GSE may represent a compelling platform for improving the viability and antioxidant properties of fish cells. |
| first_indexed | 2024-03-10T00:52:19Z |
| format | Article |
| id | doaj.art-d9e1be31d772461c9d73c131ecc053aa |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T00:52:19Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d9e1be31d772461c9d73c131ecc053aa2023-11-23T14:50:33ZengMDPI AGMolecules1420-30492022-01-0127234410.3390/molecules27020344Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in AquacultureAdriana Trapani0María Ángeles Esteban1Francesca Curci2Daniela Erminia Manno3Antonio Serra4Giuseppe Fracchiolla5Cristóbal Espinosa-Ruiz6Stefano Castellani7Massimo Conese8Department of Pharmacy-Drug Sciences, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyDepartment of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, SpainDepartment of Pharmacy-Drug Sciences, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyDipartimento di Matematica e Fisica “E. De Giorgi”, University of Salento, 73100 Lecce, ItalyDipartimento di Matematica e Fisica “E. De Giorgi”, University of Salento, 73100 Lecce, ItalyDepartment of Pharmacy-Drug Sciences, University of Bari “Aldo Moro”, Via Orabona 4, 70125 Bari, ItalyDepartment of Cell Biology and Histology, Faculty of Biology, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100 Murcia, SpainDepartment of Biomedical Sciences and Human Oncology, University of Bari “Aldo Moro”, 70124 Bari, ItalyDepartment of Medical and Surgical Sciences, University of Foggia, 71122 Foggia, ItalyThe supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed of a combination of Gelucire<sup>®</sup> 50/13 and Precirol<sup>®</sup> ATO5 to administer a grape seed extract (GSE) mixture containing several antioxidant compounds. The combination of the two lipids for the SLN formation resulted in colloids exhibiting mean particle sizes in the range 139–283 nm and zeta potential values in the range +25.6–43.4 mV. Raman spectra and X-ray diffraction evidenced structural differences between the free GSE and GSE-loaded SLN, leading to the conclusion that GSE alters the structure of the lipid nanocarriers. From a biological viewpoint, cell lines from gilthead seabream and European sea bass were exposed to different concentrations of GSE-SLN for 24 h. In general, at appropriate concentrations, GSE-SLN increased the viability of the fish cells. Furthermore, regarding the gene expression in those cells, the expression of antioxidant genes was upregulated, whereas the expression of <i>hsp70</i> and other genes related to the cytoskeleton was downregulated. Hence, an SLN formulation containing Gelucire<sup>®</sup> 50/13/Precirol<sup>®</sup> ATO5 and GSE may represent a compelling platform for improving the viability and antioxidant properties of fish cells.https://www.mdpi.com/1420-3049/27/2/344solid lipid nanoparticlesgrape seed–derived extractdrug deliveryX-ray diffractionantioxidant activityfish cells |
| spellingShingle | Adriana Trapani María Ángeles Esteban Francesca Curci Daniela Erminia Manno Antonio Serra Giuseppe Fracchiolla Cristóbal Espinosa-Ruiz Stefano Castellani Massimo Conese Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture Molecules solid lipid nanoparticles grape seed–derived extract drug delivery X-ray diffraction antioxidant activity fish cells |
| title | Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture |
| title_full | Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture |
| title_fullStr | Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture |
| title_full_unstemmed | Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture |
| title_short | Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture |
| title_sort | solid lipid nanoparticles administering antioxidant grape seed derived polyphenol compounds a potential application in aquaculture |
| topic | solid lipid nanoparticles grape seed–derived extract drug delivery X-ray diffraction antioxidant activity fish cells |
| url | https://www.mdpi.com/1420-3049/27/2/344 |
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