Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests
ObjectiveTo investigate the epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP).MethodsTotally 436 K. pneumoniae strains were collected from 7 hospitals in mainland China between 2017.01 and 2018.02...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.882210/full |
| _version_ | 1818209620742635520 |
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| author | Dakang Hu Wenjie Chen Qi Zhang Meng Li Zehua Yang Yong Wang Yunkun Huang Gang Li Dongxing Tian Pan Fu Pan Fu Weiwen Wang Ping Ren Qing Mu Lianhua Yu Xiaofei Jiang |
| author_facet | Dakang Hu Wenjie Chen Qi Zhang Meng Li Zehua Yang Yong Wang Yunkun Huang Gang Li Dongxing Tian Pan Fu Pan Fu Weiwen Wang Ping Ren Qing Mu Lianhua Yu Xiaofei Jiang |
| author_sort | Dakang Hu |
| collection | DOAJ |
| description | ObjectiveTo investigate the epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP).MethodsTotally 436 K. pneumoniae strains were collected from 7 hospitals in mainland China between 2017.01 and 2018.02. Sequence types, serotypes, antimicrobial-resistance and virulence genes were analyzed. Additionally, string test, capsule stain, Periodic Acid Schiff stain, fitness analysis, quantitative real-time PCR and mouse lethality test were also performed. Molecular combinations were used to screen putative blaKPC(+)-HvKP and Hv-blaKPC(+)-KP, followed by the confirmation of mouse lethality test.ResultsDiverse detection rates were found for the virulence genes, ranging from c-rmpA (0.0%) to entB (100.0%). According to the molecular criteria, 127, 186, 9 and 26 strains were putatively denoted as HvKP, blaKPC(+)-KP, blaKPC(+)-HvKP and Hv-blaKPC(+)-KP. Mouse lethality test confirmed 2 blaKPC(+)-HvKP strains (JS184 and TZ20) and no Hv-blaKPC(+)-KP. JS184 showed K2 serotype, thin capsule, positive exopolysaccharid and string test. TZ20 presented K20 serotype, thin capsule, negative exopolysaccharide and string test. Compared with the positive control NTUH-K2044, equal galF expression and growth curves were confirmed for JS184 and TZ20.ConclusionsMolecular determination of CR-HvKP and Hv-CRKP brings remarkable bias compared with mouse lethality test. The exact prevalence of CR-HvKP is less than 1.0%, which of Hv-CRKP is much lower. |
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| id | doaj.art-d9ea380e39ff44b2918d3a160e8ec2a3 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-12T05:03:37Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d9ea380e39ff44b2918d3a160e8ec2a32022-12-22T00:37:10ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-06-011210.3389/fcimb.2022.882210882210Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality TestsDakang Hu0Wenjie Chen1Qi Zhang2Meng Li3Zehua Yang4Yong Wang5Yunkun Huang6Gang Li7Dongxing Tian8Pan Fu9Pan Fu10Weiwen Wang11Ping Ren12Qing Mu13Lianhua Yu14Xiaofei Jiang15Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Laboratory Medicine, Henan Provincial People’s Hospital & the People’s Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Laboratory Medicine, Sixth Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, ChinaDepartment of Laboratory Medicine, Kunming Yan’an Hospital, Kunming, ChinaDepartment of Laboratory Medicine, Jinshan Hospital of Fudan University, Shanghai, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaMicrobiology Department. Children’s Hospital of Fudan University, Shanghai, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, China0Zhejiang Provincial Demonstration Centre of Laboratory Medicine Experimental Teaching, Wenzhou Medical University, Wenzhou, China1School of Pharmacy, Fudan University, Shanghai, China2Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, ChinaDepartment of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, ChinaObjectiveTo investigate the epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP).MethodsTotally 436 K. pneumoniae strains were collected from 7 hospitals in mainland China between 2017.01 and 2018.02. Sequence types, serotypes, antimicrobial-resistance and virulence genes were analyzed. Additionally, string test, capsule stain, Periodic Acid Schiff stain, fitness analysis, quantitative real-time PCR and mouse lethality test were also performed. Molecular combinations were used to screen putative blaKPC(+)-HvKP and Hv-blaKPC(+)-KP, followed by the confirmation of mouse lethality test.ResultsDiverse detection rates were found for the virulence genes, ranging from c-rmpA (0.0%) to entB (100.0%). According to the molecular criteria, 127, 186, 9 and 26 strains were putatively denoted as HvKP, blaKPC(+)-KP, blaKPC(+)-HvKP and Hv-blaKPC(+)-KP. Mouse lethality test confirmed 2 blaKPC(+)-HvKP strains (JS184 and TZ20) and no Hv-blaKPC(+)-KP. JS184 showed K2 serotype, thin capsule, positive exopolysaccharid and string test. TZ20 presented K20 serotype, thin capsule, negative exopolysaccharide and string test. Compared with the positive control NTUH-K2044, equal galF expression and growth curves were confirmed for JS184 and TZ20.ConclusionsMolecular determination of CR-HvKP and Hv-CRKP brings remarkable bias compared with mouse lethality test. The exact prevalence of CR-HvKP is less than 1.0%, which of Hv-CRKP is much lower.https://www.frontiersin.org/articles/10.3389/fcimb.2022.882210/fullcarbapenem-resistant hypervirulent Klebsiella pneumoniaehypervirulent carbapenem-resistant Klebsiella pneumoniaeepidemiologymouse lethality testhypervirulencecarbapenemase |
| spellingShingle | Dakang Hu Wenjie Chen Qi Zhang Meng Li Zehua Yang Yong Wang Yunkun Huang Gang Li Dongxing Tian Pan Fu Pan Fu Weiwen Wang Ping Ren Qing Mu Lianhua Yu Xiaofei Jiang Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests Frontiers in Cellular and Infection Microbiology carbapenem-resistant hypervirulent Klebsiella pneumoniae hypervirulent carbapenem-resistant Klebsiella pneumoniae epidemiology mouse lethality test hypervirulence carbapenemase |
| title | Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests |
| title_full | Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests |
| title_fullStr | Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests |
| title_full_unstemmed | Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests |
| title_short | Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests |
| title_sort | prevalence of carbapenem resistant hypervirulent klebsiella pneumoniae and hypervirulent carbapenem resistant klebsiella pneumoniae in china determined via mouse lethality tests |
| topic | carbapenem-resistant hypervirulent Klebsiella pneumoniae hypervirulent carbapenem-resistant Klebsiella pneumoniae epidemiology mouse lethality test hypervirulence carbapenemase |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.882210/full |
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