Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in <i>Mycoplasma genitalium</i> in Badalona, Spain
Macrolide and fluoroquinolone resistance (MLr/FQr) in <i>Mycoplasma genitalium</i> (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by p...
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MDPI AG
2022-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/4/485 |
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| author | Belén Rivaya Chloé Le Roy Elena Jordana-Lluch Gema Fernández-Rivas Cristina Casañ Victoria González Jun Hao Wang-Wang Cécile Bébéar Lurdes Matas Sabine Pereyre |
| author_facet | Belén Rivaya Chloé Le Roy Elena Jordana-Lluch Gema Fernández-Rivas Cristina Casañ Victoria González Jun Hao Wang-Wang Cécile Bébéar Lurdes Matas Sabine Pereyre |
| author_sort | Belén Rivaya |
| collection | DOAJ |
| description | Macrolide and fluoroquinolone resistance (MLr/FQr) in <i>Mycoplasma genitalium</i> (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for MLr detection in comparison with a one-step commercial assay and to assess the prevalence of MLr and FQr in Badalona, Spain. A total of 415 MG-positive samples by Allplex STI-7 (Seegene) were analyzed for MLr detection by pyrosequencing. From those, 179 samples were further analyzed for MG and MLr by ResistancePlus<sup>®</sup> MG kit (SpeeDx) and 100 of them also for fluoroquinolone resistance (FQr) by sequencing the <i>parC</i> gene. Regarding MG detection, Allplex and Resistance Plus<sup>®</sup> showed an overall agreement of 87%, but this value rose to 95.4% if we compare them for MLr detection. Prevalence of MLr was 23.1% in Badalona, but this rate increased to 73.7% in the HIV-positive patients cohort. FQr detection showed 3% of resistant strains. Pyrosequencing is a convenient and cheap technique for MLr detection, but one-step tools should be considered in high-throughput laboratories. Despite the fact that MLr remained moderate and FQr was low in our study, simultaneous MG and MLr detection would improve patient’s management applying resistance-guided treatment strategies. |
| first_indexed | 2024-03-09T11:15:24Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T11:15:24Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d9ec04eca3d44aadacdb83ab89ddbf202023-12-01T00:32:41ZengMDPI AGAntibiotics2079-63822022-04-0111448510.3390/antibiotics11040485Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in <i>Mycoplasma genitalium</i> in Badalona, SpainBelén Rivaya0Chloé Le Roy1Elena Jordana-Lluch2Gema Fernández-Rivas3Cristina Casañ4Victoria González5Jun Hao Wang-Wang6Cécile Bébéar7Lurdes Matas8Sabine Pereyre9Microbiology Department, Laboratori Clinic Metropolitana Nord, Hospital Universitari Germans Trias i Pujol, Ctra. Del Canyet, S/N, 08916 Badalona, SpainUMR 5234 Fundamental Microbiology and Pathogenicity, University of Bordeaux, CNRS, F-33000 Bordeaux, FranceMicrobiology Department, Laboratori Clinic Metropolitana Nord, Hospital Universitari Germans Trias i Pujol, Ctra. Del Canyet, S/N, 08916 Badalona, SpainMicrobiology Department, Laboratori Clinic Metropolitana Nord, Hospital Universitari Germans Trias i Pujol, Ctra. Del Canyet, S/N, 08916 Badalona, SpainMicrobiology Department, Laboratori Clinic Metropolitana Nord, Hospital Universitari Germans Trias i Pujol, Ctra. Del Canyet, S/N, 08916 Badalona, SpainMicrobiology Department, Laboratori Clinic Metropolitana Nord, Hospital Universitari Germans Trias i Pujol, Ctra. Del Canyet, S/N, 08916 Badalona, SpainMicrobiology Department, Laboratori Clinic Metropolitana Nord, Hospital Universitari Germans Trias i Pujol, Ctra. Del Canyet, S/N, 08916 Badalona, SpainUMR 5234 Fundamental Microbiology and Pathogenicity, University of Bordeaux, CNRS, F-33000 Bordeaux, FranceMicrobiology Department, Laboratori Clinic Metropolitana Nord, Hospital Universitari Germans Trias i Pujol, Ctra. Del Canyet, S/N, 08916 Badalona, SpainUMR 5234 Fundamental Microbiology and Pathogenicity, University of Bordeaux, CNRS, F-33000 Bordeaux, FranceMacrolide and fluoroquinolone resistance (MLr/FQr) in <i>Mycoplasma genitalium</i> (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for MLr detection in comparison with a one-step commercial assay and to assess the prevalence of MLr and FQr in Badalona, Spain. A total of 415 MG-positive samples by Allplex STI-7 (Seegene) were analyzed for MLr detection by pyrosequencing. From those, 179 samples were further analyzed for MG and MLr by ResistancePlus<sup>®</sup> MG kit (SpeeDx) and 100 of them also for fluoroquinolone resistance (FQr) by sequencing the <i>parC</i> gene. Regarding MG detection, Allplex and Resistance Plus<sup>®</sup> showed an overall agreement of 87%, but this value rose to 95.4% if we compare them for MLr detection. Prevalence of MLr was 23.1% in Badalona, but this rate increased to 73.7% in the HIV-positive patients cohort. FQr detection showed 3% of resistant strains. Pyrosequencing is a convenient and cheap technique for MLr detection, but one-step tools should be considered in high-throughput laboratories. Despite the fact that MLr remained moderate and FQr was low in our study, simultaneous MG and MLr detection would improve patient’s management applying resistance-guided treatment strategies.https://www.mdpi.com/2079-6382/11/4/485<i>Mycoplasma genitalium</i>macrolidefluoroquinoloneresistanceSpain |
| spellingShingle | Belén Rivaya Chloé Le Roy Elena Jordana-Lluch Gema Fernández-Rivas Cristina Casañ Victoria González Jun Hao Wang-Wang Cécile Bébéar Lurdes Matas Sabine Pereyre Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in <i>Mycoplasma genitalium</i> in Badalona, Spain Antibiotics <i>Mycoplasma genitalium</i> macrolide fluoroquinolone resistance Spain |
| title | Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in <i>Mycoplasma genitalium</i> in Badalona, Spain |
| title_full | Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in <i>Mycoplasma genitalium</i> in Badalona, Spain |
| title_fullStr | Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in <i>Mycoplasma genitalium</i> in Badalona, Spain |
| title_full_unstemmed | Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in <i>Mycoplasma genitalium</i> in Badalona, Spain |
| title_short | Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in <i>Mycoplasma genitalium</i> in Badalona, Spain |
| title_sort | detection and prevalence of macrolide and fluoroquinolone resistance in i mycoplasma genitalium i in badalona spain |
| topic | <i>Mycoplasma genitalium</i> macrolide fluoroquinolone resistance Spain |
| url | https://www.mdpi.com/2079-6382/11/4/485 |
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