Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn Kernels
Fusarium verticillioides is considered one of the most important global sources of fumonisins contamination in food and feed. Corn is one of the main commodities produced in the Northeastern Region of Brazil. The present study investigated potential mycotoxigenic fungal strains belonging to the F. v...
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MDPI AG
2016-02-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/3/54 |
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| author | Susane Chang Mariele Porto Carneiro-Leão Benny Ferreira de Oliveira Cristina Souza-Motta Nelson Lima Cledir Santos Neiva Tinti de Oliveira |
| author_facet | Susane Chang Mariele Porto Carneiro-Leão Benny Ferreira de Oliveira Cristina Souza-Motta Nelson Lima Cledir Santos Neiva Tinti de Oliveira |
| author_sort | Susane Chang |
| collection | DOAJ |
| description | Fusarium verticillioides is considered one of the most important global sources of fumonisins contamination in food and feed. Corn is one of the main commodities produced in the Northeastern Region of Brazil. The present study investigated potential mycotoxigenic fungal strains belonging to the F. verticillioides species isolated from corn kernels in 3 different Regions of the Brazilian State of Pernambuco. A polyphasic approach including classical taxonomy, molecular biology, MALDI-TOF MS and MALDI-TOF MS/MS for the identification and characterisation of the F. verticillioides strains was used. Sixty F. verticillioides strains were isolated and successfully identified by classical morphology, proteomic profiles of MALDI-TOF MS, and by molecular biology using the species-specific primers VERT-1 and VERT-2. FUM1 gene was further detected for all the 60 F. verticillioides by using the primers VERTF-1 and VERTF-2 and through the amplification profiles of the ISSR regions using the primers (GTG)5 and (GACA)4. Results obtained from molecular analysis shown a low genetic variability among these isolates from the different geographical regions. All of the 60 F. verticillioides isolates assessed by MALDI-TOF MS/MS presented ion peaks with the molecular mass of the fumonisin B1 (721.83 g/mol) and B2 (705.83 g/mol). |
| first_indexed | 2024-04-11T12:54:27Z |
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| id | doaj.art-d9f0397a5fd9434194ea25a006c3c248 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T12:54:27Z |
| publishDate | 2016-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-d9f0397a5fd9434194ea25a006c3c2482022-12-22T04:23:06ZengMDPI AGToxins2072-66512016-02-01835410.3390/toxins8030054toxins8030054Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn KernelsSusane Chang0Mariele Porto Carneiro-Leão1Benny Ferreira de Oliveira2Cristina Souza-Motta3Nelson Lima4Cledir Santos5Neiva Tinti de Oliveira6Postgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, BrazilPostgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, BrazilPostgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, BrazilPostgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, BrazilCEB-Centre of Biological Engineering, Micoteca da Universidade do Minho, University of Minho, Campus of Gualtar, 4710-057 Braga, PortugalDepartment of Chemical Sciences and Natural Resources, CIBAMA, BIOREN-UFRO, Faculty of Engineering and Sciences, Universidad de La Frontera, Av. Francisco Salazar, 01145 Temuco, ChilePostgraduate Program in Fungal Biology, Department of Mycology, Federal University of Pernambuco, Av. Prof. Nelson Chaves, s/n, Cidade Universitária, Recife 50670-420, PE, BrazilFusarium verticillioides is considered one of the most important global sources of fumonisins contamination in food and feed. Corn is one of the main commodities produced in the Northeastern Region of Brazil. The present study investigated potential mycotoxigenic fungal strains belonging to the F. verticillioides species isolated from corn kernels in 3 different Regions of the Brazilian State of Pernambuco. A polyphasic approach including classical taxonomy, molecular biology, MALDI-TOF MS and MALDI-TOF MS/MS for the identification and characterisation of the F. verticillioides strains was used. Sixty F. verticillioides strains were isolated and successfully identified by classical morphology, proteomic profiles of MALDI-TOF MS, and by molecular biology using the species-specific primers VERT-1 and VERT-2. FUM1 gene was further detected for all the 60 F. verticillioides by using the primers VERTF-1 and VERTF-2 and through the amplification profiles of the ISSR regions using the primers (GTG)5 and (GACA)4. Results obtained from molecular analysis shown a low genetic variability among these isolates from the different geographical regions. All of the 60 F. verticillioides isolates assessed by MALDI-TOF MS/MS presented ion peaks with the molecular mass of the fumonisin B1 (721.83 g/mol) and B2 (705.83 g/mol).http://www.mdpi.com/2072-6651/8/3/54mycotoxinfumonisinsmycotoxigenic fungigenetic study |
| spellingShingle | Susane Chang Mariele Porto Carneiro-Leão Benny Ferreira de Oliveira Cristina Souza-Motta Nelson Lima Cledir Santos Neiva Tinti de Oliveira Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn Kernels Toxins mycotoxin fumonisins mycotoxigenic fungi genetic study |
| title | Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn Kernels |
| title_full | Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn Kernels |
| title_fullStr | Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn Kernels |
| title_full_unstemmed | Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn Kernels |
| title_short | Polyphasic Approach Including MALDI-TOF MS/MS Analysis for Identification and Characterisation of Fusarium verticillioides in Brazilian Corn Kernels |
| title_sort | polyphasic approach including maldi tof ms ms analysis for identification and characterisation of fusarium verticillioides in brazilian corn kernels |
| topic | mycotoxin fumonisins mycotoxigenic fungi genetic study |
| url | http://www.mdpi.com/2072-6651/8/3/54 |
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