Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell
Abstract. Tcf12 has been identified as one of the main helix-loop-helix transcription factors that regulates T cell development from double negative to double positive stage transition. While, the function of Tcf12 in hematopoietic stem cells remains not investigated. In this study, we observed that...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health
2021-01-01
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| Series: | Blood Science |
| Online Access: | http://journals.lww.com/10.1097/BS9.0000000000000059 |
| _version_ | 1818312132393959424 |
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| author | Min Liao Jianwei Wang |
| author_facet | Min Liao Jianwei Wang |
| author_sort | Min Liao |
| collection | DOAJ |
| description | Abstract. Tcf12 has been identified as one of the main helix-loop-helix transcription factors that regulates T cell development from double negative to double positive stage transition. While, the function of Tcf12 in hematopoietic stem cells remains not investigated. In this study, we observed that Tcf12 is expressed in HSCs and targeted deletion of Tcf12 in hematopoietic cells results in increased frequency and absolute number of HSCs, but compromises the reconstitution capacity of HSCs. Further analysis reveals that Tcf12 is dispensable for the self-renewal of HSCs. The declined reconstituted capacity of Tcf12−/− HSCs stems from the decrease in the ability to differentiate into lymphoid-primed multipotent progenitors, and furthermore B and T lineages. |
| first_indexed | 2024-12-13T08:12:59Z |
| format | Article |
| id | doaj.art-d9f47efa5e8f4f5fa3d3e180dd9b47c9 |
| institution | Directory Open Access Journal |
| issn | 2543-6368 |
| language | English |
| last_indexed | 2024-12-13T08:12:59Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Health |
| record_format | Article |
| series | Blood Science |
| spelling | doaj.art-d9f47efa5e8f4f5fa3d3e180dd9b47c92022-12-21T23:54:10ZengWolters Kluwer HealthBlood Science2543-63682021-01-0131141910.1097/BS9.0000000000000059202101000-00003Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cellMin Liao0Jianwei Wang1School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, ChinaSchool of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, ChinaAbstract. Tcf12 has been identified as one of the main helix-loop-helix transcription factors that regulates T cell development from double negative to double positive stage transition. While, the function of Tcf12 in hematopoietic stem cells remains not investigated. In this study, we observed that Tcf12 is expressed in HSCs and targeted deletion of Tcf12 in hematopoietic cells results in increased frequency and absolute number of HSCs, but compromises the reconstitution capacity of HSCs. Further analysis reveals that Tcf12 is dispensable for the self-renewal of HSCs. The declined reconstituted capacity of Tcf12−/− HSCs stems from the decrease in the ability to differentiate into lymphoid-primed multipotent progenitors, and furthermore B and T lineages.http://journals.lww.com/10.1097/BS9.0000000000000059 |
| spellingShingle | Min Liao Jianwei Wang Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell Blood Science |
| title | Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell |
| title_full | Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell |
| title_fullStr | Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell |
| title_full_unstemmed | Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell |
| title_short | Tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell |
| title_sort | tcf12 balances the reconstitution and differentiation capacity of hematopoietic stem cell |
| url | http://journals.lww.com/10.1097/BS9.0000000000000059 |
| work_keys_str_mv | AT minliao tcf12balancesthereconstitutionanddifferentiationcapacityofhematopoieticstemcell AT jianweiwang tcf12balancesthereconstitutionanddifferentiationcapacityofhematopoieticstemcell |