Associations between Single Nucleotide Polymorphisms from the Genes of Chemokines and the CXCR2 Chemokine Receptor and an Increased Risk of Endometrial Cancer
Significant relationships with endometrial cancer were demonstrated, both for <i>CCL2</i>, <i>CCL5</i>, and <i>CXCL8</i> chemokines and for the chemokine receptor <i>CXCR2</i>. The reported case-control study of genetic associations was designed to est...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/22/5416 |
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| author | Wioletta Izabela Wujcicka Agnieszka Zając Krzysztof Szyłło Hanna Romanowicz Beata Smolarz Grzegorz Stachowiak |
| author_facet | Wioletta Izabela Wujcicka Agnieszka Zając Krzysztof Szyłło Hanna Romanowicz Beata Smolarz Grzegorz Stachowiak |
| author_sort | Wioletta Izabela Wujcicka |
| collection | DOAJ |
| description | Significant relationships with endometrial cancer were demonstrated, both for <i>CCL2</i>, <i>CCL5</i>, and <i>CXCL8</i> chemokines and for the chemokine receptor <i>CXCR2</i>. The reported case-control study of genetic associations was designed to establish the role of selected single nucleotide polymorphisms (SNPs) of the <i>CCL2</i>, <i>CCL5</i>, <i>CXCL8</i>, and <i>CXCR2</i> genes in the onset and progression of endometrial cancer. This study was conducted on 282 women, including 132 (46.8%) patients with endometrial cancer and 150 (53.2%) non-cancerous controls. The genotypes for <i>CCL2</i> rs4586, <i>CCL5</i> rs2107538 and rs2280789, <i>CXCL8</i> rs2227532 and −738 T>A, and <i>CXCR2</i> rs1126580 were determined, using PCR-RFLP assays. The AA homozygotes in <i>CCL5</i> rs2107538 were associated with more than a quadruple risk of endometrial cancer (<i>p</i> ≤ 0.050). The GA heterozygotes in the <i>CXCR2</i> SNP were associated with approximately threefold higher cancer risk (<i>p</i> ≤ 0.001). That association also remained significant after certain adjustments, carried out for age, diabetes mellitus, arterial hypertension, or endometrial thickness above 5 mm (<i>p</i> ≤ 0.050). The A-A haplotypes for the <i>CCL5</i> polymorphisms and T-A-A haplotypes for the <i>CCL2</i> and <i>CCL5</i> SNPs were associated with about a twofold risk of endometrial cancer (<i>p</i> ≤ 0.050). In conclusion, <i>CCL2</i> rs4586, <i>CCL5</i> rs2107538 and rs2280789, and <i>CXCR2</i> rs1126580 demonstrated significant associations with an increased risk of endometrial cancer. |
| first_indexed | 2024-03-09T16:57:07Z |
| format | Article |
| id | doaj.art-d9f7a5a8793747408e5126211daf2384 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T16:57:07Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-d9f7a5a8793747408e5126211daf23842023-11-24T14:34:21ZengMDPI AGCancers2072-66942023-11-011522541610.3390/cancers15225416Associations between Single Nucleotide Polymorphisms from the Genes of Chemokines and the CXCR2 Chemokine Receptor and an Increased Risk of Endometrial CancerWioletta Izabela Wujcicka0Agnieszka Zając1Krzysztof Szyłło2Hanna Romanowicz3Beata Smolarz4Grzegorz Stachowiak5Scientific Laboratory of the Center of Medical Laboratory Diagnostics and Screening, Polish Mother’s Memorial Hospital—Research Institute, 93-338 Lodz, PolandDepartment of Operative Gynecology and Gynecologic Oncology, Polish Mother’s Memorial Hospital—Research Institute, 93-338 Lodz, PolandDepartment of Operative Gynecology and Gynecologic Oncology, Polish Mother’s Memorial Hospital—Research Institute, 93-338 Lodz, PolandDepartment of Clinical Pathomorphology, Polish Mother’s Memorial Hospital—Research Institute, 93-338 Lodz, PolandLaboratory of Cancer Genetics of the Department of Clinical Pathomorphology, Polish Mother’s Memorial Hospital—Research Institute, 93-338 Lodz, PolandDepartment of Operative Gynecology and Gynecologic Oncology, Polish Mother’s Memorial Hospital—Research Institute, 93-338 Lodz, PolandSignificant relationships with endometrial cancer were demonstrated, both for <i>CCL2</i>, <i>CCL5</i>, and <i>CXCL8</i> chemokines and for the chemokine receptor <i>CXCR2</i>. The reported case-control study of genetic associations was designed to establish the role of selected single nucleotide polymorphisms (SNPs) of the <i>CCL2</i>, <i>CCL5</i>, <i>CXCL8</i>, and <i>CXCR2</i> genes in the onset and progression of endometrial cancer. This study was conducted on 282 women, including 132 (46.8%) patients with endometrial cancer and 150 (53.2%) non-cancerous controls. The genotypes for <i>CCL2</i> rs4586, <i>CCL5</i> rs2107538 and rs2280789, <i>CXCL8</i> rs2227532 and −738 T>A, and <i>CXCR2</i> rs1126580 were determined, using PCR-RFLP assays. The AA homozygotes in <i>CCL5</i> rs2107538 were associated with more than a quadruple risk of endometrial cancer (<i>p</i> ≤ 0.050). The GA heterozygotes in the <i>CXCR2</i> SNP were associated with approximately threefold higher cancer risk (<i>p</i> ≤ 0.001). That association also remained significant after certain adjustments, carried out for age, diabetes mellitus, arterial hypertension, or endometrial thickness above 5 mm (<i>p</i> ≤ 0.050). The A-A haplotypes for the <i>CCL5</i> polymorphisms and T-A-A haplotypes for the <i>CCL2</i> and <i>CCL5</i> SNPs were associated with about a twofold risk of endometrial cancer (<i>p</i> ≤ 0.050). In conclusion, <i>CCL2</i> rs4586, <i>CCL5</i> rs2107538 and rs2280789, and <i>CXCR2</i> rs1126580 demonstrated significant associations with an increased risk of endometrial cancer.https://www.mdpi.com/2072-6694/15/22/5416endometrial cancerchemokineschemokine receptorsingle nucleotide polymorphisms (SNPs)<i>CCL2</i><i>CCL5</i> |
| spellingShingle | Wioletta Izabela Wujcicka Agnieszka Zając Krzysztof Szyłło Hanna Romanowicz Beata Smolarz Grzegorz Stachowiak Associations between Single Nucleotide Polymorphisms from the Genes of Chemokines and the CXCR2 Chemokine Receptor and an Increased Risk of Endometrial Cancer Cancers endometrial cancer chemokines chemokine receptor single nucleotide polymorphisms (SNPs) <i>CCL2</i> <i>CCL5</i> |
| title | Associations between Single Nucleotide Polymorphisms from the Genes of Chemokines and the CXCR2 Chemokine Receptor and an Increased Risk of Endometrial Cancer |
| title_full | Associations between Single Nucleotide Polymorphisms from the Genes of Chemokines and the CXCR2 Chemokine Receptor and an Increased Risk of Endometrial Cancer |
| title_fullStr | Associations between Single Nucleotide Polymorphisms from the Genes of Chemokines and the CXCR2 Chemokine Receptor and an Increased Risk of Endometrial Cancer |
| title_full_unstemmed | Associations between Single Nucleotide Polymorphisms from the Genes of Chemokines and the CXCR2 Chemokine Receptor and an Increased Risk of Endometrial Cancer |
| title_short | Associations between Single Nucleotide Polymorphisms from the Genes of Chemokines and the CXCR2 Chemokine Receptor and an Increased Risk of Endometrial Cancer |
| title_sort | associations between single nucleotide polymorphisms from the genes of chemokines and the cxcr2 chemokine receptor and an increased risk of endometrial cancer |
| topic | endometrial cancer chemokines chemokine receptor single nucleotide polymorphisms (SNPs) <i>CCL2</i> <i>CCL5</i> |
| url | https://www.mdpi.com/2072-6694/15/22/5416 |
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