Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives
Methanol is becoming an attractive fermentation feedstock for large-scale bioproduction of chemicals, due to its natural abundance and mature production technology. Native methylotrophs, which can utilize methanol as the only source of carbon and energy, are ideal hosts for methanol bioconversion du...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2023-06-01
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| Series: | Green Chemical Engineering |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666952822000863 |
| _version_ | 1827934037992275968 |
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| author | Jing Wang Ruirui Qin Yuanke Guo Chen Ma Xin Wang Kequan Chen Pingkai Ouyang |
| author_facet | Jing Wang Ruirui Qin Yuanke Guo Chen Ma Xin Wang Kequan Chen Pingkai Ouyang |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Methanol is becoming an attractive fermentation feedstock for large-scale bioproduction of chemicals, due to its natural abundance and mature production technology. Native methylotrophs, which can utilize methanol as the only source of carbon and energy, are ideal hosts for methanol bioconversion due to their high methanol utilization rate and have been extensively employed in the production of value-added chemicals from methanol. Here, we review the natural methanol utilization pathways in native methylotrophs, describing the available synthetic biology tools developed for engineering native methylotrophs, and discuss the strategies for improving their methanol utilization efficiency. Finally, the representative examples of engineering the native methylotrophs to produce value-added products from methanol are summarized. Furthermore, we also discuss the major challenges and possible solutions for the application of native methylotrophs in methanol-based biomanufacturing. |
| first_indexed | 2024-03-13T07:32:57Z |
| format | Article |
| id | doaj.art-d9f8d54e6aaa49bebefa2329841331e1 |
| institution | Directory Open Access Journal |
| issn | 2666-9528 |
| language | English |
| last_indexed | 2024-03-13T07:32:57Z |
| publishDate | 2023-06-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Green Chemical Engineering |
| spelling | doaj.art-d9f8d54e6aaa49bebefa2329841331e12023-06-04T04:24:34ZengKeAi Communications Co. Ltd.Green Chemical Engineering2666-95282023-06-0142199211Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectivesJing Wang0Ruirui Qin1Yuanke Guo2Chen Ma3Xin Wang4Kequan Chen5Pingkai Ouyang6State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, ChinaCorresponding author.; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, ChinaCorresponding author.; State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, ChinaState Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, ChinaMethanol is becoming an attractive fermentation feedstock for large-scale bioproduction of chemicals, due to its natural abundance and mature production technology. Native methylotrophs, which can utilize methanol as the only source of carbon and energy, are ideal hosts for methanol bioconversion due to their high methanol utilization rate and have been extensively employed in the production of value-added chemicals from methanol. Here, we review the natural methanol utilization pathways in native methylotrophs, describing the available synthetic biology tools developed for engineering native methylotrophs, and discuss the strategies for improving their methanol utilization efficiency. Finally, the representative examples of engineering the native methylotrophs to produce value-added products from methanol are summarized. Furthermore, we also discuss the major challenges and possible solutions for the application of native methylotrophs in methanol-based biomanufacturing.http://www.sciencedirect.com/science/article/pii/S2666952822000863Native methylotrophsMethanolBiomanufacturingValue-added chemicals |
| spellingShingle | Jing Wang Ruirui Qin Yuanke Guo Chen Ma Xin Wang Kequan Chen Pingkai Ouyang Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives Green Chemical Engineering Native methylotrophs Methanol Biomanufacturing Value-added chemicals |
| title | Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives |
| title_full | Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives |
| title_fullStr | Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives |
| title_full_unstemmed | Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives |
| title_short | Engineering the native methylotrophs for the bioconversion of methanol to value-added chemicals: current status and future perspectives |
| title_sort | engineering the native methylotrophs for the bioconversion of methanol to value added chemicals current status and future perspectives |
| topic | Native methylotrophs Methanol Biomanufacturing Value-added chemicals |
| url | http://www.sciencedirect.com/science/article/pii/S2666952822000863 |
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