Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract
This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydra...
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| Format: | Article |
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MDPI AG
2014-09-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/9/14567 |
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| author | Neelesh Sharma Kalpa W. Samarakoon Rajendra Gyawali Yang-Ho Park Sung-Jin Lee Sung Jong Oh Tae-Hoon Lee Dong Kee Jeong |
| author_facet | Neelesh Sharma Kalpa W. Samarakoon Rajendra Gyawali Yang-Ho Park Sung-Jin Lee Sung Jong Oh Tae-Hoon Lee Dong Kee Jeong |
| author_sort | Neelesh Sharma |
| collection | DOAJ |
| description | This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The E. hirta ethanol extract (0.5 mg/mL) exhibited DPPH-scavenging activity of 61.19% ± 0.22%, while the positive control (0.5 mg/mL ascorbic acid) had 100% ± 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC50) was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 µg/mL E. hirta extract, and nitric oxide production was decreased significantly (p < 0.05). The extract also showed selective anticancer activity at a concentration of 100 µg/mL (p < 0.05). These results indicated that E. hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications. |
| first_indexed | 2024-04-13T21:07:05Z |
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| id | doaj.art-d9fb8fc254764c03a5edb11991cb5474 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T21:07:05Z |
| publishDate | 2014-09-01 |
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| series | Molecules |
| spelling | doaj.art-d9fb8fc254764c03a5edb11991cb54742022-12-22T02:29:57ZengMDPI AGMolecules1420-30492014-09-01199145671458110.3390/molecules190914567molecules190914567Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic ExtractNeelesh Sharma0Kalpa W. Samarakoon1Rajendra Gyawali2Yang-Ho Park3Sung-Jin Lee4Sung Jong Oh5Tae-Hoon Lee6Dong Kee Jeong7Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju 690-756, KoreaDepartment of Marine Life Science, Jeju National University, Jeju 690-756, KoreaFaculty of Biotechnology, School of Applied Life Science, Jeju National University, Jeju 690-756, KoreaBRM Institute, Seoul 135-010, KoreaDepartment of Animal Biotechnology, School of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, KoreaDepartment of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju 690-756, KoreaDepartment of Oral Biochemistry, Dental Science Research Institute, Chonnam National University, Gwangju 500-757, KoreaDepartment of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju 690-756, KoreaThis study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The E. hirta ethanol extract (0.5 mg/mL) exhibited DPPH-scavenging activity of 61.19% ± 0.22%, while the positive control (0.5 mg/mL ascorbic acid) had 100% ± 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC50) was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 µg/mL E. hirta extract, and nitric oxide production was decreased significantly (p < 0.05). The extract also showed selective anticancer activity at a concentration of 100 µg/mL (p < 0.05). These results indicated that E. hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications.http://www.mdpi.com/1420-3049/19/9/14567Euphorbia hirtachemical compositiononline HPLC-ABTS+• bioassayantioxidantanti-inflammatoryanticancer |
| spellingShingle | Neelesh Sharma Kalpa W. Samarakoon Rajendra Gyawali Yang-Ho Park Sung-Jin Lee Sung Jong Oh Tae-Hoon Lee Dong Kee Jeong Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract Molecules Euphorbia hirta chemical composition online HPLC-ABTS+• bioassay antioxidant anti-inflammatory anticancer |
| title | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
| title_full | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
| title_fullStr | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
| title_full_unstemmed | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
| title_short | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
| title_sort | evaluation of the antioxidant anti inflammatory and anticancer activities of euphorbia hirta ethanolic extract |
| topic | Euphorbia hirta chemical composition online HPLC-ABTS+• bioassay antioxidant anti-inflammatory anticancer |
| url | http://www.mdpi.com/1420-3049/19/9/14567 |
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