Large-scale comparative analysis of cytogenetic markers across Lepidoptera
Abstract Fluorescence in situ hybridization (FISH) allows identification of particular chromosomes and their rearrangements. Using FISH with signal enhancement via antibody amplification and enzymatically catalysed reporter deposition, we evaluated applicability of universal cytogenetic markers, nam...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-91665-7 |
| _version_ | 1818431288648925184 |
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| author | Irena Provazníková Martina Hejníčková Sander Visser Martina Dalíková Leonela Z. Carabajal Paladino Magda Zrzavá Anna Voleníková František Marec Petr Nguyen |
| author_facet | Irena Provazníková Martina Hejníčková Sander Visser Martina Dalíková Leonela Z. Carabajal Paladino Magda Zrzavá Anna Voleníková František Marec Petr Nguyen |
| author_sort | Irena Provazníková |
| collection | DOAJ |
| description | Abstract Fluorescence in situ hybridization (FISH) allows identification of particular chromosomes and their rearrangements. Using FISH with signal enhancement via antibody amplification and enzymatically catalysed reporter deposition, we evaluated applicability of universal cytogenetic markers, namely 18S and 5S rDNA genes, U1 and U2 snRNA genes, and histone H3 genes, in the study of the karyotype evolution in moths and butterflies. Major rDNA underwent rather erratic evolution, which does not always reflect chromosomal changes. In contrast, the hybridization pattern of histone H3 genes was well conserved, reflecting the stable organisation of lepidopteran genomes. Unlike 5S rDNA and U1 and U2 snRNA genes which we failed to detect, except for 5S rDNA in a few representatives of early diverging lepidopteran lineages. To explain the negative FISH results, we used quantitative PCR and Southern hybridization to estimate the copy number and organization of the studied genes in selected species. The results suggested that their detection was hampered by long spacers between the genes and/or their scattered distribution. Our results question homology of 5S rDNA and U1 and U2 snRNA loci in comparative studies. We recommend the use of histone H3 in studies of karyotype evolution. |
| first_indexed | 2024-12-14T15:46:56Z |
| format | Article |
| id | doaj.art-d9fc6e1164074ab38557b6519ce0705f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:46:56Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-d9fc6e1164074ab38557b6519ce0705f2022-12-21T22:55:28ZengNature PortfolioScientific Reports2045-23222021-06-0111111310.1038/s41598-021-91665-7Large-scale comparative analysis of cytogenetic markers across LepidopteraIrena Provazníková0Martina Hejníčková1Sander Visser2Martina Dalíková3Leonela Z. Carabajal Paladino4Magda Zrzavá5Anna Voleníková6František Marec7Petr Nguyen8Faculty of Science, University of South BohemiaFaculty of Science, University of South BohemiaFaculty of Science, University of South BohemiaFaculty of Science, University of South BohemiaThe Pirbright InstituteFaculty of Science, University of South BohemiaFaculty of Science, University of South BohemiaInstitute of Entomology, Biology Centre CASFaculty of Science, University of South BohemiaAbstract Fluorescence in situ hybridization (FISH) allows identification of particular chromosomes and their rearrangements. Using FISH with signal enhancement via antibody amplification and enzymatically catalysed reporter deposition, we evaluated applicability of universal cytogenetic markers, namely 18S and 5S rDNA genes, U1 and U2 snRNA genes, and histone H3 genes, in the study of the karyotype evolution in moths and butterflies. Major rDNA underwent rather erratic evolution, which does not always reflect chromosomal changes. In contrast, the hybridization pattern of histone H3 genes was well conserved, reflecting the stable organisation of lepidopteran genomes. Unlike 5S rDNA and U1 and U2 snRNA genes which we failed to detect, except for 5S rDNA in a few representatives of early diverging lepidopteran lineages. To explain the negative FISH results, we used quantitative PCR and Southern hybridization to estimate the copy number and organization of the studied genes in selected species. The results suggested that their detection was hampered by long spacers between the genes and/or their scattered distribution. Our results question homology of 5S rDNA and U1 and U2 snRNA loci in comparative studies. We recommend the use of histone H3 in studies of karyotype evolution.https://doi.org/10.1038/s41598-021-91665-7 |
| spellingShingle | Irena Provazníková Martina Hejníčková Sander Visser Martina Dalíková Leonela Z. Carabajal Paladino Magda Zrzavá Anna Voleníková František Marec Petr Nguyen Large-scale comparative analysis of cytogenetic markers across Lepidoptera Scientific Reports |
| title | Large-scale comparative analysis of cytogenetic markers across Lepidoptera |
| title_full | Large-scale comparative analysis of cytogenetic markers across Lepidoptera |
| title_fullStr | Large-scale comparative analysis of cytogenetic markers across Lepidoptera |
| title_full_unstemmed | Large-scale comparative analysis of cytogenetic markers across Lepidoptera |
| title_short | Large-scale comparative analysis of cytogenetic markers across Lepidoptera |
| title_sort | large scale comparative analysis of cytogenetic markers across lepidoptera |
| url | https://doi.org/10.1038/s41598-021-91665-7 |
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