Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae)
<p>Measuring stress responses is an important aspect for the conservation of endangered wild species. Non-invasive measuring of glucocorticoid metabolite levels has become an important tool to measure stress intensity. The aims of the present study were as follows: to validate the enzyme imm...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wildlife Information Liaison Development Society
2015-05-01
|
| Series: | Journal of Threatened Taxa |
| Subjects: | |
| Online Access: | http://threatenedtaxa.org/index.php/JoTT/article/view/2020 |
| _version_ | 1818023915304255488 |
|---|---|
| author | Cristiane Schilbach Pizzutto Manuela Gonçalves Fraga Geronymo Sgai Cláudia Pereda Francischini Priscila Viau Cláudio Alvarenga de Oliveira Marcelo Alcindo de Barros Vaz Guimarães |
| author_facet | Cristiane Schilbach Pizzutto Manuela Gonçalves Fraga Geronymo Sgai Cláudia Pereda Francischini Priscila Viau Cláudio Alvarenga de Oliveira Marcelo Alcindo de Barros Vaz Guimarães |
| author_sort | Cristiane Schilbach Pizzutto |
| collection | DOAJ |
| description | <p>Measuring stress responses is an important aspect for the conservation of endangered wild species. Non-invasive measuring of glucocorticoid metabolite levels has become an important tool to measure stress intensity. The aims of the present study were as follows: to validate the enzyme immunoassay to measure the concentration of fecal metabolites of glucocorticoids (FGM) after stressful stimuli and to determine whether FGM concentrations fluctuate diurnally in Black-tufted Marmosets <em>Callithrix penicillata</em> in captivity. Eight captive healthy adult Black-tufted Marmosets (four males and four females) were included in the study. The animals were subjected to three treatments: (1) hormone challenge with adrenocorticotropic hormone (ACTH), (2) saline administration and (3) control treatment to monitor diurnal changes of FGM. Fecal samples were collected on days -1, 0, +1 and +2, with intramuscular administration of ACTH and saline performed on day 0. To control diurnal variations, all feces from all animals were collected over six consecutive days and identified using the time of defecation and animal identification number. There were four designated two-hour periods per day (8–10 h, 10–12 h, 12–14 h and 14–16 h), and the samples were grouped for each two-hour period to obtain a representative pool. The samples were frozen, and the metabolite concentrations were measured by enzyme immunoassay following extraction. The results show that immunoassay measurements of FGM concentrations in <em>C. penicillata </em>can be validated physiologically. Diurnal variation of the FGM concentration was observed, with significantly increased FGM levels in the early afternoon in both sexes. The mean FGM concentration was higher in captive females than in males. Physical restraint followed by saline administration led to adrenocortical stimulation similar to that observed following ACTH hormone challenge, a finding that has not previously been reported in <em>C. penicillata</em>. Our results show immunoassay measurements of FGM concentrations provide a valuable tool for the non-invasive study of the endocrine correlates of behavior and well-being of this species.</p><div> </div> |
| first_indexed | 2024-12-10T03:51:54Z |
| format | Article |
| id | doaj.art-da01313ab5994663b9c56e68cb66d110 |
| institution | Directory Open Access Journal |
| issn | 0974-7893 0974-7907 |
| language | English |
| last_indexed | 2024-12-10T03:51:54Z |
| publishDate | 2015-05-01 |
| publisher | Wildlife Information Liaison Development Society |
| record_format | Article |
| series | Journal of Threatened Taxa |
| spelling | doaj.art-da01313ab5994663b9c56e68cb66d1102022-12-22T02:03:15ZengWildlife Information Liaison Development SocietyJournal of Threatened Taxa0974-78930974-79072015-05-01767234724210.11609/JoTT.o4099.7234-421707Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae)Cristiane Schilbach Pizzutto0Manuela Gonçalves Fraga Geronymo Sgai1Cláudia Pereda Francischini2Priscila Viau3Cláudio Alvarenga de Oliveira4Marcelo Alcindo de Barros Vaz Guimarães5Department of Animal Reproduction - Faculty of Veterinary Medicine and Zootechny - University of São Paulo - Brazil. Avenida Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 055508-270, BrazilDepartment of Animal Reproduction - Faculty of Veterinary Medicine and Zootechny - University of São Paulo - Brazil. Avenida Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 055508-270, BrazilDepartment of Animal Reproduction - Faculty of Veterinary Medicine and Zootechny - University of São Paulo - Brazil. Avenida Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 055508-270, BraziDepartment of Animal Reproduction - Faculty of Veterinary Medicine and Zootechny - University of São Paulo - Brazil. Avenida Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 055508-270, BrazilDepartment of Animal Reproduction - Faculty of Veterinary Medicine and Zootechny - University of São Paulo - Brazil. Avenida Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 055508-270, BrazilDepartment of Animal Reproduction - Faculty of Veterinary Medicine and Zootechny - University of São Paulo - Brazil. Avenida Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP 055508-270, Brazil<p>Measuring stress responses is an important aspect for the conservation of endangered wild species. Non-invasive measuring of glucocorticoid metabolite levels has become an important tool to measure stress intensity. The aims of the present study were as follows: to validate the enzyme immunoassay to measure the concentration of fecal metabolites of glucocorticoids (FGM) after stressful stimuli and to determine whether FGM concentrations fluctuate diurnally in Black-tufted Marmosets <em>Callithrix penicillata</em> in captivity. Eight captive healthy adult Black-tufted Marmosets (four males and four females) were included in the study. The animals were subjected to three treatments: (1) hormone challenge with adrenocorticotropic hormone (ACTH), (2) saline administration and (3) control treatment to monitor diurnal changes of FGM. Fecal samples were collected on days -1, 0, +1 and +2, with intramuscular administration of ACTH and saline performed on day 0. To control diurnal variations, all feces from all animals were collected over six consecutive days and identified using the time of defecation and animal identification number. There were four designated two-hour periods per day (8–10 h, 10–12 h, 12–14 h and 14–16 h), and the samples were grouped for each two-hour period to obtain a representative pool. The samples were frozen, and the metabolite concentrations were measured by enzyme immunoassay following extraction. The results show that immunoassay measurements of FGM concentrations in <em>C. penicillata </em>can be validated physiologically. Diurnal variation of the FGM concentration was observed, with significantly increased FGM levels in the early afternoon in both sexes. The mean FGM concentration was higher in captive females than in males. Physical restraint followed by saline administration led to adrenocortical stimulation similar to that observed following ACTH hormone challenge, a finding that has not previously been reported in <em>C. penicillata</em>. Our results show immunoassay measurements of FGM concentrations provide a valuable tool for the non-invasive study of the endocrine correlates of behavior and well-being of this species.</p><div> </div>http://threatenedtaxa.org/index.php/JoTT/article/view/2020Callithrix penicillatadiurnal variationfecal glucocorticoid metabolitesstressvalidation. |
| spellingShingle | Cristiane Schilbach Pizzutto Manuela Gonçalves Fraga Geronymo Sgai Cláudia Pereda Francischini Priscila Viau Cláudio Alvarenga de Oliveira Marcelo Alcindo de Barros Vaz Guimarães Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae) Journal of Threatened Taxa Callithrix penicillata diurnal variation fecal glucocorticoid metabolites stress validation. |
| title | Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae) |
| title_full | Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae) |
| title_fullStr | Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae) |
| title_full_unstemmed | Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae) |
| title_short | Physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive Black-tufted Marmoset Callithrix penicillata (Mammalia: Primates: Callitrichidae) |
| title_sort | physiological validation of enzyme immunoassay of fecal glucocorticoid metabolite levels and diurnal variation measured in captive black tufted marmoset callithrix penicillata mammalia primates callitrichidae |
| topic | Callithrix penicillata diurnal variation fecal glucocorticoid metabolites stress validation. |
| url | http://threatenedtaxa.org/index.php/JoTT/article/view/2020 |
| work_keys_str_mv | AT cristianeschilbachpizzutto physiologicalvalidationofenzymeimmunoassayoffecalglucocorticoidmetabolitelevelsanddiurnalvariationmeasuredincaptiveblacktuftedmarmosetcallithrixpenicillatamammaliaprimatescallitrichidae AT manuelagoncalvesfragageronymosgai physiologicalvalidationofenzymeimmunoassayoffecalglucocorticoidmetabolitelevelsanddiurnalvariationmeasuredincaptiveblacktuftedmarmosetcallithrixpenicillatamammaliaprimatescallitrichidae AT claudiaperedafrancischini physiologicalvalidationofenzymeimmunoassayoffecalglucocorticoidmetabolitelevelsanddiurnalvariationmeasuredincaptiveblacktuftedmarmosetcallithrixpenicillatamammaliaprimatescallitrichidae AT priscilaviau physiologicalvalidationofenzymeimmunoassayoffecalglucocorticoidmetabolitelevelsanddiurnalvariationmeasuredincaptiveblacktuftedmarmosetcallithrixpenicillatamammaliaprimatescallitrichidae AT claudioalvarengadeoliveira physiologicalvalidationofenzymeimmunoassayoffecalglucocorticoidmetabolitelevelsanddiurnalvariationmeasuredincaptiveblacktuftedmarmosetcallithrixpenicillatamammaliaprimatescallitrichidae AT marceloalcindodebarrosvazguimaraes physiologicalvalidationofenzymeimmunoassayoffecalglucocorticoidmetabolitelevelsanddiurnalvariationmeasuredincaptiveblacktuftedmarmosetcallithrixpenicillatamammaliaprimatescallitrichidae |