Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil
An anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), 28S rRNA and ITS1, 5.8S and ITS2 regions were amplified using the polymeras...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200209&lng=en&tlng=en |
| _version_ | 1828816681688891392 |
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| author | Cláudio Mafra Cynthia Mantovani Juliana Novo Borges Rafael Mazioli Barcelos Cláudia Portes Santos |
| author_facet | Cláudio Mafra Cynthia Mantovani Juliana Novo Borges Rafael Mazioli Barcelos Cláudia Portes Santos |
| author_sort | Cláudio Mafra |
| collection | DOAJ |
| description | An anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), 28S rRNA and ITS1, 5.8S and ITS2 regions were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships of the larva. The genetic profile confirmed that this larva belongs to the species Pseudoterranova decipiens (sensu stricto). This is the first molecular and ultrastructural study of Pseudoterranova decipiens (sensu stricto) in imported cod sold in Brazil. The health implications of these findings are discussed. |
| first_indexed | 2024-12-12T11:08:54Z |
| format | Article |
| id | doaj.art-da01dcb20b3f4b1d881e88cd3293967c |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-12T11:08:54Z |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-da01dcb20b3f4b1d881e88cd3293967c2022-12-22T00:26:21ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-296124220921510.1590/S1984-29612015037S1984-29612015000200209Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in BrazilCláudio MafraCynthia MantovaniJuliana Novo BorgesRafael Mazioli BarcelosCláudia Portes SantosAn anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), 28S rRNA and ITS1, 5.8S and ITS2 regions were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships of the larva. The genetic profile confirmed that this larva belongs to the species Pseudoterranova decipiens (sensu stricto). This is the first molecular and ultrastructural study of Pseudoterranova decipiens (sensu stricto) in imported cod sold in Brazil. The health implications of these findings are discussed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200209&lng=en&tlng=enAnisakiasiscodwormfood safetypublic health |
| spellingShingle | Cláudio Mafra Cynthia Mantovani Juliana Novo Borges Rafael Mazioli Barcelos Cláudia Portes Santos Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil Revista Brasileira de Parasitologia Veterinária Anisakiasis codworm food safety public health |
| title | Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil |
| title_full | Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil |
| title_fullStr | Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil |
| title_full_unstemmed | Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil |
| title_short | Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil |
| title_sort | morphological and molecular diagnosis of pseudoterranova decipiens sensu stricto anisakidae in imported cod sold in brazil |
| topic | Anisakiasis codworm food safety public health |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200209&lng=en&tlng=en |
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