AKT family and miRNAs expression in IL-2-induced CD4+T cells
Objective(s): Study of non-coding RNAs is considerable to elucidate principal biological questions or design new therapeutic strategies. miRNAs are a group of non-coding RNAs that their functions in PI3K/AKT signaling and apoptosis pathways after T cell activation is not entirely clear. Herein, miRN...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2014-11-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_3740_e90652b47de04422958e2d67863ce080.html |
| _version_ | 1811297579572396032 |
|---|---|
| author | Najmeh Ranji Majid Sadeghizadeh Morteza Karimipoor Mohammad Ali Shokrgozar Reza Ebrahimzadeh-Vesal |
| author_facet | Najmeh Ranji Majid Sadeghizadeh Morteza Karimipoor Mohammad Ali Shokrgozar Reza Ebrahimzadeh-Vesal |
| author_sort | Najmeh Ranji |
| collection | DOAJ |
| description | Objective(s): Study of non-coding RNAs is considerable to elucidate principal biological questions or design new therapeutic strategies. miRNAs are a group of non-coding RNAs that their functions in PI3K/AKT signaling and apoptosis pathways after T cell activation is not entirely clear. Herein, miRNAs expression and their putative targets in the mentioned pathways were studied in the activated CD4+T cells.
Materials and Methods: Herein, proliferation rate and IL-2 secretion were measured in treated and untreated cells by IL-2. Putative targets of up-regulated miRNAs were predicted by bioinformatics approaches in the apoptotic and PI3K/AKT signaling pathways. Then the expression of two putative targets was evaluated by quantitative RT-PCR.
Results: Proliferation rate of treated cells by IL-2 increased in a dose- and time- dependent manner. Naive and activated CD4+T cells induced by different dose of IL-2 secreted abundant amounts of IL-2. Also, in IL-2 un-induced cells (IL-2 depleted cells) after 3 days, decrease of proliferation has been shown. In silico analysis predicted putative targets of up-regulated miRNAs such as AKT1, AKT3 and apoptotic genes in the activated cells induced or un-induced by IL-2. Decrease of AKT3 was shown by Q-RT-PCR as a potential target of miRNAs overexpressed in IL-2 depleted cells. But there was no significant difference in AKT1 expression in two cell groups.
Conclusion: Our analysis suggests that decrease of AKT3 was likely controlled via up-regulation of specific miRNAs in IL-2 depleted cells. Also it seems that miRNAs play role in induction of different apoptosis pathways in IL-2 induced and un-induced cells. |
| first_indexed | 2024-04-13T06:07:26Z |
| format | Article |
| id | doaj.art-da049f08b58e4e0781d3592c47f06954 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T06:07:26Z |
| publishDate | 2014-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-da049f08b58e4e0781d3592c47f069542022-12-22T02:59:13ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742014-11-0117118868943740AKT family and miRNAs expression in IL-2-induced CD4+T cellsNajmeh Ranji0Majid Sadeghizadeh1Morteza Karimipoor2Mohammad Ali Shokrgozar3Reza Ebrahimzadeh-Vesal4Department of Genetics, College of Science, Rasht Branch, Islamic Azad University, Rasht, IranDepartment of Genetics, School of Biological Sciences, Tarbiat Modares University, Tehran, IranDepartment of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IranNational Cell Bank of Iran, Pasteur Institute of Iran, Tehran, IranDepartment of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IranObjective(s): Study of non-coding RNAs is considerable to elucidate principal biological questions or design new therapeutic strategies. miRNAs are a group of non-coding RNAs that their functions in PI3K/AKT signaling and apoptosis pathways after T cell activation is not entirely clear. Herein, miRNAs expression and their putative targets in the mentioned pathways were studied in the activated CD4+T cells. Materials and Methods: Herein, proliferation rate and IL-2 secretion were measured in treated and untreated cells by IL-2. Putative targets of up-regulated miRNAs were predicted by bioinformatics approaches in the apoptotic and PI3K/AKT signaling pathways. Then the expression of two putative targets was evaluated by quantitative RT-PCR. Results: Proliferation rate of treated cells by IL-2 increased in a dose- and time- dependent manner. Naive and activated CD4+T cells induced by different dose of IL-2 secreted abundant amounts of IL-2. Also, in IL-2 un-induced cells (IL-2 depleted cells) after 3 days, decrease of proliferation has been shown. In silico analysis predicted putative targets of up-regulated miRNAs such as AKT1, AKT3 and apoptotic genes in the activated cells induced or un-induced by IL-2. Decrease of AKT3 was shown by Q-RT-PCR as a potential target of miRNAs overexpressed in IL-2 depleted cells. But there was no significant difference in AKT1 expression in two cell groups. Conclusion: Our analysis suggests that decrease of AKT3 was likely controlled via up-regulation of specific miRNAs in IL-2 depleted cells. Also it seems that miRNAs play role in induction of different apoptosis pathways in IL-2 induced and un-induced cells.http://ijbms.mums.ac.ir/pdf_3740_e90652b47de04422958e2d67863ce080.htmlAKT3 IL-2 induction In silico analysis miRNAs |
| spellingShingle | Najmeh Ranji Majid Sadeghizadeh Morteza Karimipoor Mohammad Ali Shokrgozar Reza Ebrahimzadeh-Vesal AKT family and miRNAs expression in IL-2-induced CD4+T cells Iranian Journal of Basic Medical Sciences AKT3 IL-2 induction In silico analysis miRNAs |
| title | AKT family and miRNAs expression in IL-2-induced CD4+T cells |
| title_full | AKT family and miRNAs expression in IL-2-induced CD4+T cells |
| title_fullStr | AKT family and miRNAs expression in IL-2-induced CD4+T cells |
| title_full_unstemmed | AKT family and miRNAs expression in IL-2-induced CD4+T cells |
| title_short | AKT family and miRNAs expression in IL-2-induced CD4+T cells |
| title_sort | akt family and mirnas expression in il 2 induced cd4 t cells |
| topic | AKT3 IL-2 induction In silico analysis miRNAs |
| url | http://ijbms.mums.ac.ir/pdf_3740_e90652b47de04422958e2d67863ce080.html |
| work_keys_str_mv | AT najmehranji aktfamilyandmirnasexpressioninil2inducedcd4tcells AT majidsadeghizadeh aktfamilyandmirnasexpressioninil2inducedcd4tcells AT mortezakarimipoor aktfamilyandmirnasexpressioninil2inducedcd4tcells AT mohammadalishokrgozar aktfamilyandmirnasexpressioninil2inducedcd4tcells AT rezaebrahimzadehvesal aktfamilyandmirnasexpressioninil2inducedcd4tcells |