Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor
The micrographic phytopathogen Botrytis cinerea causes gray mold diseases in a large number of dicotyledonous crop plants and ornamentals. Colonization of host tissue is accompanied by rapid killing of plant cells ahead of the growing hyphen, probably caused by secretion of nonspecific phytotoxins,...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2005-06-01
|
| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0602 |
| _version_ | 1818915197766598656 |
|---|---|
| author | Verena Siewers Muriel Viaud Daniel Jimenez-Teja Isidro G. Collado Christian Schulze Gronover Jean-Marc Pradier Bettina Tudzynsk Paul Tudzynski |
| author_facet | Verena Siewers Muriel Viaud Daniel Jimenez-Teja Isidro G. Collado Christian Schulze Gronover Jean-Marc Pradier Bettina Tudzynsk Paul Tudzynski |
| author_sort | Verena Siewers |
| collection | DOAJ |
| description | The micrographic phytopathogen Botrytis cinerea causes gray mold diseases in a large number of dicotyledonous crop plants and ornamentals. Colonization of host tissue is accompanied by rapid killing of plant cells ahead of the growing hyphen, probably caused by secretion of nonspecific phytotoxins, e.g., the sesquiterpene botrydial. Although all pathogenic strains tested so far had been shown to secrete botrydial and although the toxin causes comparable necrotic lesions as infection by the fungus, the role of botrydial in the infection process has not been elucidated so far. Here, we describe the functional characterization of bcbot1, encoding a P450 monooxygenase and provide evidence that it is involved in the botrydial pathway, i.e., it represents the first botrydial biosynthetic gene identified. We show that bcbot1 is expressed in planta and that expression in vitro and in planta is controlled by an α-subunit of a heterotrimeric GTP-binding protein, BCG1. Deletion of bcbot1 in three standard strains of B. cinerea shows that the effect on virulence (on several host plants) is strain-dependent; only deletion in one of the strains (T4) led to reduced virulence. |
| first_indexed | 2024-12-19T23:58:27Z |
| format | Article |
| id | doaj.art-da050c517b1c4271b9dc0bce3724faeb |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:58:27Z |
| publishDate | 2005-06-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-da050c517b1c4271b9dc0bce3724faeb2022-12-21T20:00:55ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062005-06-0118660261210.1094/MPMI-18-0602Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence FactorVerena SiewersMuriel ViaudDaniel Jimenez-TejaIsidro G. ColladoChristian Schulze GronoverJean-Marc PradierBettina TudzynskPaul TudzynskiThe micrographic phytopathogen Botrytis cinerea causes gray mold diseases in a large number of dicotyledonous crop plants and ornamentals. Colonization of host tissue is accompanied by rapid killing of plant cells ahead of the growing hyphen, probably caused by secretion of nonspecific phytotoxins, e.g., the sesquiterpene botrydial. Although all pathogenic strains tested so far had been shown to secrete botrydial and although the toxin causes comparable necrotic lesions as infection by the fungus, the role of botrydial in the infection process has not been elucidated so far. Here, we describe the functional characterization of bcbot1, encoding a P450 monooxygenase and provide evidence that it is involved in the botrydial pathway, i.e., it represents the first botrydial biosynthetic gene identified. We show that bcbot1 is expressed in planta and that expression in vitro and in planta is controlled by an α-subunit of a heterotrimeric GTP-binding protein, BCG1. Deletion of bcbot1 in three standard strains of B. cinerea shows that the effect on virulence (on several host plants) is strain-dependent; only deletion in one of the strains (T4) led to reduced virulence.https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0602secondary metabolismsignaling |
| spellingShingle | Verena Siewers Muriel Viaud Daniel Jimenez-Teja Isidro G. Collado Christian Schulze Gronover Jean-Marc Pradier Bettina Tudzynsk Paul Tudzynski Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor Molecular Plant-Microbe Interactions secondary metabolism signaling |
| title | Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor |
| title_full | Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor |
| title_fullStr | Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor |
| title_full_unstemmed | Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor |
| title_short | Functional Analysis of the Cytochrome P450 Monooxygenase Gene bcbot1 of Botrytis cinerea Indicates That Botrydial Is a Strain-Specific Virulence Factor |
| title_sort | functional analysis of the cytochrome p450 monooxygenase gene bcbot1 of botrytis cinerea indicates that botrydial is a strain specific virulence factor |
| topic | secondary metabolism signaling |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-18-0602 |
| work_keys_str_mv | AT verenasiewers functionalanalysisofthecytochromep450monooxygenasegenebcbot1ofbotrytiscinereaindicatesthatbotrydialisastrainspecificvirulencefactor AT murielviaud functionalanalysisofthecytochromep450monooxygenasegenebcbot1ofbotrytiscinereaindicatesthatbotrydialisastrainspecificvirulencefactor AT danieljimenezteja functionalanalysisofthecytochromep450monooxygenasegenebcbot1ofbotrytiscinereaindicatesthatbotrydialisastrainspecificvirulencefactor AT isidrogcollado functionalanalysisofthecytochromep450monooxygenasegenebcbot1ofbotrytiscinereaindicatesthatbotrydialisastrainspecificvirulencefactor AT christianschulzegronover functionalanalysisofthecytochromep450monooxygenasegenebcbot1ofbotrytiscinereaindicatesthatbotrydialisastrainspecificvirulencefactor AT jeanmarcpradier functionalanalysisofthecytochromep450monooxygenasegenebcbot1ofbotrytiscinereaindicatesthatbotrydialisastrainspecificvirulencefactor AT bettinatudzynsk functionalanalysisofthecytochromep450monooxygenasegenebcbot1ofbotrytiscinereaindicatesthatbotrydialisastrainspecificvirulencefactor AT paultudzynski functionalanalysisofthecytochromep450monooxygenasegenebcbot1ofbotrytiscinereaindicatesthatbotrydialisastrainspecificvirulencefactor |