Characterization of Coagulase Positive and Negative Staphylococci Recovered from Mastitic Cows and Buffaloes
This study aimed to characterize the different Staphylococci recovered from mastitic cows and buffaloes. A total of 126 mastitis milk samples were aseptically collected from clinically mastitic animals including 87 cows and 39 buffaloes. Bacteriological examination and biochemical identification usi...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Beni-Suef University
2022-04-01
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| Series: | Journal of Veterinary Medical Research |
| Subjects: | |
| Online Access: | https://jvmr.journals.ekb.eg/article_228736_3aa24f26a9001cabef26e36f178e6f66.pdf |
| _version_ | 1818523543436001280 |
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| author | Ismail Raheel Asmaa Nady Mohammed; Asmaa Abdrabo Mohamed |
| author_facet | Ismail Raheel Asmaa Nady Mohammed; Asmaa Abdrabo Mohamed |
| author_sort | Ismail Raheel |
| collection | DOAJ |
| description | This study aimed to characterize the different Staphylococci recovered from mastitic cows and buffaloes. A total of 126 mastitis milk samples were aseptically collected from clinically mastitic animals including 87 cows and 39 buffaloes. Bacteriological examination and biochemical identification using VITEK-2-compact-SYSTEM revealed that a total of 94 Staphylococcus isolates (74.6%) were recovered; 56 isolates (59.6%) and 38 isolates (40%) from cows and buffaloes, respectively. S. aureus was the most predominant isolate (n=26; 15 from cows and 11 from buffaloes) with a percentage of 27.7%. Moreover, 68 coagulase-negative staphylococci (CNS) isolates (72.3%) were identified of which; 21 S. epidermidis (22.3%); all isolates were from cattle, followed by 18 S. lentus (19.1%); 8 and 10 from cows and buffaloes, respectively, 17 S. simulans (18%); 6 and 11 isolates, respectively, and finally 12 S. hominis (12.9%); 5 and 7 isolates, respectively. Antimicrobial susceptibility testing showed that all isolates were sensitive to ceftriaxone, ciprofloxacin, ofloxacin and sulfamethoxazole-trimethoprim. On the contrary, all isolates were resistant to penicillin and streptomycin. Multidrug resistance (MDR) was detected in 21 (22.3%) Staphylococci isolates. Biofilm formation capacity was phenotypically assessed on YESCA CR agar medium and showed that all Staphylococci isolates were curli-producing. Application of PCR technique revealed that sed, seb genes were the most prevalent genes in all isolates, followed by fnbA gene which was detected in 80% of the isolates, and then mecA, blaZ, and icaA with percentages of 60%, 40%, and 40%, respectively. |
| first_indexed | 2024-12-11T05:46:11Z |
| format | Article |
| id | doaj.art-da06aa78cd05404ea5e39bba97bdf59b |
| institution | Directory Open Access Journal |
| issn | 2357-0512 2357-0520 |
| language | English |
| last_indexed | 2024-12-11T05:46:11Z |
| publishDate | 2022-04-01 |
| publisher | Faculty of Veterinary Medicine, Beni-Suef University |
| record_format | Article |
| series | Journal of Veterinary Medical Research |
| spelling | doaj.art-da06aa78cd05404ea5e39bba97bdf59b2022-12-22T01:18:58ZengFaculty of Veterinary Medicine, Beni-Suef UniversityJournal of Veterinary Medical Research2357-05122357-05202022-04-012901612Characterization of Coagulase Positive and Negative Staphylococci Recovered from Mastitic Cows and BuffaloesIsmail Raheel0Asmaa Nady Mohammed;1 Asmaa Abdrabo Mohamed2Bacteriology, Mycology, and Immunology Department, Faculty of Veterinary Medicine Beni-suef University, Beni-Suef 62511, Egypt.Department of Hygiene, Zoonoses, and Epidemiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.Directorate of Veterinary Medicine, El-Fayoum Governorate, Egypt.This study aimed to characterize the different Staphylococci recovered from mastitic cows and buffaloes. A total of 126 mastitis milk samples were aseptically collected from clinically mastitic animals including 87 cows and 39 buffaloes. Bacteriological examination and biochemical identification using VITEK-2-compact-SYSTEM revealed that a total of 94 Staphylococcus isolates (74.6%) were recovered; 56 isolates (59.6%) and 38 isolates (40%) from cows and buffaloes, respectively. S. aureus was the most predominant isolate (n=26; 15 from cows and 11 from buffaloes) with a percentage of 27.7%. Moreover, 68 coagulase-negative staphylococci (CNS) isolates (72.3%) were identified of which; 21 S. epidermidis (22.3%); all isolates were from cattle, followed by 18 S. lentus (19.1%); 8 and 10 from cows and buffaloes, respectively, 17 S. simulans (18%); 6 and 11 isolates, respectively, and finally 12 S. hominis (12.9%); 5 and 7 isolates, respectively. Antimicrobial susceptibility testing showed that all isolates were sensitive to ceftriaxone, ciprofloxacin, ofloxacin and sulfamethoxazole-trimethoprim. On the contrary, all isolates were resistant to penicillin and streptomycin. Multidrug resistance (MDR) was detected in 21 (22.3%) Staphylococci isolates. Biofilm formation capacity was phenotypically assessed on YESCA CR agar medium and showed that all Staphylococci isolates were curli-producing. Application of PCR technique revealed that sed, seb genes were the most prevalent genes in all isolates, followed by fnbA gene which was detected in 80% of the isolates, and then mecA, blaZ, and icaA with percentages of 60%, 40%, and 40%, respectively.https://jvmr.journals.ekb.eg/article_228736_3aa24f26a9001cabef26e36f178e6f66.pdfbiofilmcoagulase negative staphylococcimastitic cows and buffaloesresistance and virulence geness. aureus |
| spellingShingle | Ismail Raheel Asmaa Nady Mohammed; Asmaa Abdrabo Mohamed Characterization of Coagulase Positive and Negative Staphylococci Recovered from Mastitic Cows and Buffaloes Journal of Veterinary Medical Research biofilm coagulase negative staphylococci mastitic cows and buffaloes resistance and virulence genes s. aureus |
| title | Characterization of Coagulase Positive and Negative Staphylococci Recovered from Mastitic Cows and Buffaloes |
| title_full | Characterization of Coagulase Positive and Negative Staphylococci Recovered from Mastitic Cows and Buffaloes |
| title_fullStr | Characterization of Coagulase Positive and Negative Staphylococci Recovered from Mastitic Cows and Buffaloes |
| title_full_unstemmed | Characterization of Coagulase Positive and Negative Staphylococci Recovered from Mastitic Cows and Buffaloes |
| title_short | Characterization of Coagulase Positive and Negative Staphylococci Recovered from Mastitic Cows and Buffaloes |
| title_sort | characterization of coagulase positive and negative staphylococci recovered from mastitic cows and buffaloes |
| topic | biofilm coagulase negative staphylococci mastitic cows and buffaloes resistance and virulence genes s. aureus |
| url | https://jvmr.journals.ekb.eg/article_228736_3aa24f26a9001cabef26e36f178e6f66.pdf |
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