Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant Metastases
Background: The aim of this study was to determine the regional heterogeneity and clinicopathological significance of microRNA-21 (miR-21) in advanced colorectal cancer (CRC) patients with distant metastasis. Methods: miR-21 expression was investigated by using locked nucleic acid– fluorescence in s...
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| Format: | Article |
| Language: | English |
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Korean Society of Pathologists & the Korean Society for Cytopathology
2016-07-01
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| Series: | Journal of Pathology and Translational Medicine |
| Subjects: | |
| Online Access: | http://www.jpatholtm.org/upload/pdf/jptm-2016-03-19.pdf |
| _version_ | 1818953603178561536 |
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| author | Kyu Sang Lee Soo Kyung Nam Jiwon Koh Duck-Woo Kim Sung-Bum Kang Gheeyoung Choe Woo Ho Kim Hye Seung Lee |
| author_facet | Kyu Sang Lee Soo Kyung Nam Jiwon Koh Duck-Woo Kim Sung-Bum Kang Gheeyoung Choe Woo Ho Kim Hye Seung Lee |
| author_sort | Kyu Sang Lee |
| collection | DOAJ |
| description | Background: The aim of this study was to determine the regional heterogeneity and clinicopathological significance of microRNA-21 (miR-21) in advanced colorectal cancer (CRC) patients with distant metastasis. Methods: miR-21 expression was investigated by using locked nucleic acid– fluorescence in situ hybridization in the center and periphery of the primary cancer and in distant metastasis from 170 patients with advanced CRC. In addition, α-smooth muscle actin and desmin were evaluated to identify cancer-associated fibroblasts (CAFs) by using immunohistochemistry. Results: The miR-21 signal was observed in the cancer stroma. The expression of miR-21 (a score of 1–4) in the center and periphery of the primary cancer and in distant metastasis was observed in specimens from 133 (78.2%), 105 (61.8%), and 91 (53.5%) patients, respectively. miR-21 expression was heterogeneous in advanced CRC. Discordance between miR-21 expression in the center of the primary cancer and either the periphery of the primary cancer or distant metastasis was 31.7% or 44.7%, respectively. miR-21 stromal expression in the periphery of the primary cancer was significantly associated with a better prognosis (p=.004). miR-21 expression was significantly associated with CAFs in the center of the primary cancer (p=.001) and distant metastases (p=.041). Conclusions: miR-21 expression is observed in cancer stroma related to the CAF quantity and frequently presents regional heterogeneity in CRC. Our findings indicate that the role of miR-21 in predicting prognosis may be controversial but provide a new perspective of miR-21 level measurement in cancer specimens. |
| first_indexed | 2024-12-20T10:08:54Z |
| format | Article |
| id | doaj.art-da0a3abad0064ceaa9ed5b8a668721ca |
| institution | Directory Open Access Journal |
| issn | 2383-7837 2383-7845 |
| language | English |
| last_indexed | 2024-12-20T10:08:54Z |
| publishDate | 2016-07-01 |
| publisher | Korean Society of Pathologists & the Korean Society for Cytopathology |
| record_format | Article |
| series | Journal of Pathology and Translational Medicine |
| spelling | doaj.art-da0a3abad0064ceaa9ed5b8a668721ca2022-12-21T19:44:12ZengKorean Society of Pathologists & the Korean Society for CytopathologyJournal of Pathology and Translational Medicine2383-78372383-78452016-07-0150427027710.4132/jptm.2016.03.1916614Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant MetastasesKyu Sang Lee0Soo Kyung Nam1Jiwon Koh2Duck-Woo Kim3Sung-Bum Kang4Gheeyoung Choe5Woo Ho Kim6Hye Seung Lee7 Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea Department of Pathology, Seoul National University College of Medicine, Seoul, Korea Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Korea Department of Surgery, Seoul National University Bundang Hospital, Seongnam, Korea Department of Pathology, Seoul National University Bundang Hospital, Seongnam, Korea Department of Pathology, Seoul National University College of Medicine, Seoul, Korea Department of Pathology, Seoul National University Bundang Hospital, Seongnam, KoreaBackground: The aim of this study was to determine the regional heterogeneity and clinicopathological significance of microRNA-21 (miR-21) in advanced colorectal cancer (CRC) patients with distant metastasis. Methods: miR-21 expression was investigated by using locked nucleic acid– fluorescence in situ hybridization in the center and periphery of the primary cancer and in distant metastasis from 170 patients with advanced CRC. In addition, α-smooth muscle actin and desmin were evaluated to identify cancer-associated fibroblasts (CAFs) by using immunohistochemistry. Results: The miR-21 signal was observed in the cancer stroma. The expression of miR-21 (a score of 1–4) in the center and periphery of the primary cancer and in distant metastasis was observed in specimens from 133 (78.2%), 105 (61.8%), and 91 (53.5%) patients, respectively. miR-21 expression was heterogeneous in advanced CRC. Discordance between miR-21 expression in the center of the primary cancer and either the periphery of the primary cancer or distant metastasis was 31.7% or 44.7%, respectively. miR-21 stromal expression in the periphery of the primary cancer was significantly associated with a better prognosis (p=.004). miR-21 expression was significantly associated with CAFs in the center of the primary cancer (p=.001) and distant metastases (p=.041). Conclusions: miR-21 expression is observed in cancer stroma related to the CAF quantity and frequently presents regional heterogeneity in CRC. Our findings indicate that the role of miR-21 in predicting prognosis may be controversial but provide a new perspective of miR-21 level measurement in cancer specimens.http://www.jpatholtm.org/upload/pdf/jptm-2016-03-19.pdfColorectal neoplasmsMicroRNA-21Neoplasm metastasisGenetic heterogeneity |
| spellingShingle | Kyu Sang Lee Soo Kyung Nam Jiwon Koh Duck-Woo Kim Sung-Bum Kang Gheeyoung Choe Woo Ho Kim Hye Seung Lee Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant Metastases Journal of Pathology and Translational Medicine Colorectal neoplasms MicroRNA-21 Neoplasm metastasis Genetic heterogeneity |
| title | Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant Metastases |
| title_full | Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant Metastases |
| title_fullStr | Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant Metastases |
| title_full_unstemmed | Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant Metastases |
| title_short | Stromal Expression of MicroRNA-21 in Advanced Colorectal Cancer Patients with Distant Metastases |
| title_sort | stromal expression of microrna 21 in advanced colorectal cancer patients with distant metastases |
| topic | Colorectal neoplasms MicroRNA-21 Neoplasm metastasis Genetic heterogeneity |
| url | http://www.jpatholtm.org/upload/pdf/jptm-2016-03-19.pdf |
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