Bioactive extracts of Gentiana asclepiadea: antioxidant, antimicrobial, and antibiofilm activity
Extracts of the aerial parts and roots of the wild-growing medicinal plant Gentiana asclepiadea were analysed for their antimicrobial, antibiofilm, and antioxidant activity with quantification of the total phenolic and total flavonoid content. Antimicrobial activity was tested against pathogenic and...
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| Format: | Article |
| Language: | English |
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University of Belgrade
2018-10-01
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| Series: | Botanica Serbica |
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| Online Access: | http://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2018_42_2_720_full.pdf |
| _version_ | 1818579121133846528 |
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| author | Stefanović Olgica Ličina Braho Vasić Sava Radojević Ivana Čomić Ljiljana |
| author_facet | Stefanović Olgica Ličina Braho Vasić Sava Radojević Ivana Čomić Ljiljana |
| author_sort | Stefanović Olgica |
| collection | DOAJ |
| description | Extracts of the aerial parts and roots of the wild-growing medicinal plant Gentiana asclepiadea were analysed for their antimicrobial, antibiofilm, and antioxidant activity with quantification of the total phenolic and total flavonoid content. Antimicrobial activity was tested against pathogenic and spoilage bacteria, yeasts, and moulds using the microdilution method. The strongest antibacterial activity was detected on Bacillus species, where minimum inhibitory concentrations (MICs) of from 0.16 mg/mL to 5 mg/mL were obtained, while antifungal activity was low to moderate, with MICs between 1.25 and 20 mg/mL. In the crystal violet assay, the extracts inhibit 50% biofilm formation in the concentration range of from 2.12 to 37.04 mg/mL. Staphylococcus aureus, S. aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853 biofilms were the most sensitive to the presence of extracts. The extracts rich in phenolic compounds showed good DPPH-scavenging activity, with EC50 values between 181.3 and 614.3 μg/mL for extracts of aerial parts and from 426.67 to >1000 μg/mL for root extracts. Even though G. asclepiadea has long been traditionally used, its biological activity is still insufficiently explored, so the obtained results are significant for contributing new knowledge about the plant's medicinal properties. |
| first_indexed | 2024-12-16T06:56:40Z |
| format | Article |
| id | doaj.art-da0f1beef80e4cb1bbc4a0402f4ce593 |
| institution | Directory Open Access Journal |
| issn | 1821-2158 1821-2638 |
| language | English |
| last_indexed | 2024-12-16T06:56:40Z |
| publishDate | 2018-10-01 |
| publisher | University of Belgrade |
| record_format | Article |
| series | Botanica Serbica |
| spelling | doaj.art-da0f1beef80e4cb1bbc4a0402f4ce5932022-12-21T22:40:17ZengUniversity of BelgradeBotanica Serbica1821-21581821-26382018-10-0142222322910.5281/zenodo.1468319Bioactive extracts of Gentiana asclepiadea: antioxidant, antimicrobial, and antibiofilm activityStefanović Olgica0Ličina Braho1Vasić Sava2Radojević Ivana3Čomić Ljiljana4Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of SerbiaDepartment of Biomedical Sciences, State University of Novi Pazar, Vuka Karadžića bb, 36300 Novi Pazar, Republic of SerbiaDepartment of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of SerbiaDepartment of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of SerbiaDepartment of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovića 12, 34000 Kragujevac, Republic of SerbiaExtracts of the aerial parts and roots of the wild-growing medicinal plant Gentiana asclepiadea were analysed for their antimicrobial, antibiofilm, and antioxidant activity with quantification of the total phenolic and total flavonoid content. Antimicrobial activity was tested against pathogenic and spoilage bacteria, yeasts, and moulds using the microdilution method. The strongest antibacterial activity was detected on Bacillus species, where minimum inhibitory concentrations (MICs) of from 0.16 mg/mL to 5 mg/mL were obtained, while antifungal activity was low to moderate, with MICs between 1.25 and 20 mg/mL. In the crystal violet assay, the extracts inhibit 50% biofilm formation in the concentration range of from 2.12 to 37.04 mg/mL. Staphylococcus aureus, S. aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853 biofilms were the most sensitive to the presence of extracts. The extracts rich in phenolic compounds showed good DPPH-scavenging activity, with EC50 values between 181.3 and 614.3 μg/mL for extracts of aerial parts and from 426.67 to >1000 μg/mL for root extracts. Even though G. asclepiadea has long been traditionally used, its biological activity is still insufficiently explored, so the obtained results are significant for contributing new knowledge about the plant's medicinal properties.http://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2018_42_2_720_full.pdfGentiana asclepiadeaextractsantimicrobial agentsantioxidantsbiofilm |
| spellingShingle | Stefanović Olgica Ličina Braho Vasić Sava Radojević Ivana Čomić Ljiljana Bioactive extracts of Gentiana asclepiadea: antioxidant, antimicrobial, and antibiofilm activity Botanica Serbica Gentiana asclepiadea extracts antimicrobial agents antioxidants biofilm |
| title | Bioactive extracts of Gentiana asclepiadea: antioxidant, antimicrobial, and antibiofilm activity |
| title_full | Bioactive extracts of Gentiana asclepiadea: antioxidant, antimicrobial, and antibiofilm activity |
| title_fullStr | Bioactive extracts of Gentiana asclepiadea: antioxidant, antimicrobial, and antibiofilm activity |
| title_full_unstemmed | Bioactive extracts of Gentiana asclepiadea: antioxidant, antimicrobial, and antibiofilm activity |
| title_short | Bioactive extracts of Gentiana asclepiadea: antioxidant, antimicrobial, and antibiofilm activity |
| title_sort | bioactive extracts of gentiana asclepiadea antioxidant antimicrobial and antibiofilm activity |
| topic | Gentiana asclepiadea extracts antimicrobial agents antioxidants biofilm |
| url | http://botanicaserbica.bio.bg.ac.rs/arhiva/pdf/2018_42_2_720_full.pdf |
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