Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic Yak
Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone ER...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Asian-Australasian Association of Animal Production Societies
2014-12-01
|
| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-27-12-1684.pdf |
| _version_ | 1828231019402100736 |
|---|---|
| author | Mei Fu Xian-Rong Xiong Dao-liang Lan Jian Li |
| author_facet | Mei Fu Xian-Rong Xiong Dao-liang Lan Jian Li |
| author_sort | Mei Fu |
| collection | DOAJ |
| description | Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone ERα and ERβ genes. Bioinformatics methods were used to analyze the evolutionary relationship between yaks and other species, and real-time PCR was performed to identify the mRNA expression of ERα and ERβ. Sequence analysis showed that the ER open reading frames (ORFs) encoded 596 and 527 amino acid proteins. The yak ERα and ERβ shared 45.3% to 99.5% and 53.9% to 99.1% protein sequence identities with other species homologs, respectively. Real-time PCR analysis revealed that ERα and ERβ were expressed in a variety of tissues, but the expression level of ERα was higher than that of ERβ in all tissues, except testis. The mRNA expression of ERα was highest in the mammary gland, followed by uterus, oviduct, and ovary, and lowest in the liver, kidney, lung, testis, spleen, and heart. The ERβ mRNA level was highest in the ovary; intermediary in the uterus and oviduct; and lowest in the heart, liver, spleen, lung, kidney, mammary gland, and testis. The identification and tissue distribution of ER genes in yaks provides a foundation for the further study on their biological functions. |
| first_indexed | 2024-04-12T18:57:10Z |
| format | Article |
| id | doaj.art-da0f9ad8c4fa4f1993d43ce0c880a73b |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-04-12T18:57:10Z |
| publishDate | 2014-12-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-da0f9ad8c4fa4f1993d43ce0c880a73b2022-12-22T03:20:17ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172014-12-0127121684169010.5713/ajas.2014.1438022978Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic YakMei Fu0Xian-Rong Xiong1Dao-liang Lan2Jian Li3 College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, China Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu, Sichuan 610041, .China College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan 610041, ChinaEstrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone ERα and ERβ genes. Bioinformatics methods were used to analyze the evolutionary relationship between yaks and other species, and real-time PCR was performed to identify the mRNA expression of ERα and ERβ. Sequence analysis showed that the ER open reading frames (ORFs) encoded 596 and 527 amino acid proteins. The yak ERα and ERβ shared 45.3% to 99.5% and 53.9% to 99.1% protein sequence identities with other species homologs, respectively. Real-time PCR analysis revealed that ERα and ERβ were expressed in a variety of tissues, but the expression level of ERα was higher than that of ERβ in all tissues, except testis. The mRNA expression of ERα was highest in the mammary gland, followed by uterus, oviduct, and ovary, and lowest in the liver, kidney, lung, testis, spleen, and heart. The ERβ mRNA level was highest in the ovary; intermediary in the uterus and oviduct; and lowest in the heart, liver, spleen, lung, kidney, mammary gland, and testis. The identification and tissue distribution of ER genes in yaks provides a foundation for the further study on their biological functions.http://www.ajas.info/upload/pdf/ajas-27-12-1684.pdfYakEstrogen Receptor GeneGene ExpressionTissue Distribution |
| spellingShingle | Mei Fu Xian-Rong Xiong Dao-liang Lan Jian Li Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic Yak Asian-Australasian Journal of Animal Sciences Yak Estrogen Receptor Gene Gene Expression Tissue Distribution |
| title | Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic Yak |
| title_full | Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic Yak |
| title_fullStr | Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic Yak |
| title_full_unstemmed | Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic Yak |
| title_short | Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic Yak |
| title_sort | molecular characterization and tissue distribution of estrogen receptor genes in domestic yak |
| topic | Yak Estrogen Receptor Gene Gene Expression Tissue Distribution |
| url | http://www.ajas.info/upload/pdf/ajas-27-12-1684.pdf |
| work_keys_str_mv | AT meifu molecularcharacterizationandtissuedistributionofestrogenreceptorgenesindomesticyak AT xianrongxiong molecularcharacterizationandtissuedistributionofestrogenreceptorgenesindomesticyak AT daolianglan molecularcharacterizationandtissuedistributionofestrogenreceptorgenesindomesticyak AT jianli molecularcharacterizationandtissuedistributionofestrogenreceptorgenesindomesticyak |