Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection
BackgroundAs the largest plant receptor-like protein kinase (RLK) superfamily, the leucine-rich repeat receptor-like kinases (LRR-RLKs) family are involved in plant growth, development, and stress responses. However, the functions of LRR-RLKs in wheat immunity remain unknown.ResultsIn the current st...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1109845/full |
| _version_ | 1811169003209490432 |
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| author | Shuang Liu Shuang Liu Shuang Liu Jiajia Lei Jiajia Lei Juan Zhang Juan Zhang Hanhong Liu Zhuangxin Ye Zhuangxin Ye Jin Yang Jin Yang Qiseng Lu Qiseng Lu Peng Liu Peng Liu Jianping Chen Jianping Chen Jian Yang Jian Yang |
| author_facet | Shuang Liu Shuang Liu Shuang Liu Jiajia Lei Jiajia Lei Juan Zhang Juan Zhang Hanhong Liu Zhuangxin Ye Zhuangxin Ye Jin Yang Jin Yang Qiseng Lu Qiseng Lu Peng Liu Peng Liu Jianping Chen Jianping Chen Jian Yang Jian Yang |
| author_sort | Shuang Liu |
| collection | DOAJ |
| description | BackgroundAs the largest plant receptor-like protein kinase (RLK) superfamily, the leucine-rich repeat receptor-like kinases (LRR-RLKs) family are involved in plant growth, development, and stress responses. However, the functions of LRR-RLKs in wheat immunity remain unknown.ResultsIn the current study, 929 LRR-RLKs were identified in Triticum aestivum genome database using the BLAST and hidden Markov models (HMM) approach and divided into 14 clades. Chromosomal localization and synteny analysis revealed that TaLRR-RLKs were randomly distributed on all chromosomes with 921 collinear events. Through the cis-acting elements analysis, we observed that TaLRR-RLKs participated in hormone response, light response, development, metabolism, and response to environmental stress. The transcript level of 14 random selected TaLRR-RLKs from each subfamily was regulated by plant hormone treatment and Chinese wheat mosaic virus (CWMV) infection. The function of TaLRR-RLKs in wheat resistance to CWMV infection was further investigated by virus-induced gene silencing assay. Additionally, the accumulation of MeJA response genes, as well as CWMV RNA were not changed in the TaLRR-RLK silencing plants under MeJA treatment.ConclusionsOur results demonstrated that TaLRR-RLKs play an important role in wheat resistance to viral infection via hormone signals and lay the groundwork for the functional study of TaLRR-RLKs in wheat. |
| first_indexed | 2024-04-10T16:35:03Z |
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| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T16:35:03Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-da0fa11110a64df7b2867c0ab60ac1cd2023-02-08T14:23:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.11098451109845Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infectionShuang Liu0Shuang Liu1Shuang Liu2Jiajia Lei3Jiajia Lei4Juan Zhang5Juan Zhang6Hanhong Liu7Zhuangxin Ye8Zhuangxin Ye9Jin Yang10Jin Yang11Qiseng Lu12Qiseng Lu13Peng Liu14Peng Liu15Jianping Chen16Jianping Chen17Jian Yang18Jian Yang19College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaJunan County Bureau of Agriculture and Country, Linyi, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, ChinaKey Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, ChinaBackgroundAs the largest plant receptor-like protein kinase (RLK) superfamily, the leucine-rich repeat receptor-like kinases (LRR-RLKs) family are involved in plant growth, development, and stress responses. However, the functions of LRR-RLKs in wheat immunity remain unknown.ResultsIn the current study, 929 LRR-RLKs were identified in Triticum aestivum genome database using the BLAST and hidden Markov models (HMM) approach and divided into 14 clades. Chromosomal localization and synteny analysis revealed that TaLRR-RLKs were randomly distributed on all chromosomes with 921 collinear events. Through the cis-acting elements analysis, we observed that TaLRR-RLKs participated in hormone response, light response, development, metabolism, and response to environmental stress. The transcript level of 14 random selected TaLRR-RLKs from each subfamily was regulated by plant hormone treatment and Chinese wheat mosaic virus (CWMV) infection. The function of TaLRR-RLKs in wheat resistance to CWMV infection was further investigated by virus-induced gene silencing assay. Additionally, the accumulation of MeJA response genes, as well as CWMV RNA were not changed in the TaLRR-RLK silencing plants under MeJA treatment.ConclusionsOur results demonstrated that TaLRR-RLKs play an important role in wheat resistance to viral infection via hormone signals and lay the groundwork for the functional study of TaLRR-RLKs in wheat.https://www.frontiersin.org/articles/10.3389/fpls.2022.1109845/fullLRR-RLKswheatgenome-wideCWMVresistanceplant hormone |
| spellingShingle | Shuang Liu Shuang Liu Shuang Liu Jiajia Lei Jiajia Lei Juan Zhang Juan Zhang Hanhong Liu Zhuangxin Ye Zhuangxin Ye Jin Yang Jin Yang Qiseng Lu Qiseng Lu Peng Liu Peng Liu Jianping Chen Jianping Chen Jian Yang Jian Yang Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection Frontiers in Plant Science LRR-RLKs wheat genome-wide CWMV resistance plant hormone |
| title | Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection |
| title_full | Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection |
| title_fullStr | Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection |
| title_full_unstemmed | Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection |
| title_short | Genome-wide identification and analysis of wheat LRR-RLK family genes following Chinese wheat mosaic virus infection |
| title_sort | genome wide identification and analysis of wheat lrr rlk family genes following chinese wheat mosaic virus infection |
| topic | LRR-RLKs wheat genome-wide CWMV resistance plant hormone |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1109845/full |
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