Analysis of CDR3 Sequences from T-Cell Receptor β in Acute Respiratory Distress Syndrome
Acute Respiratory Distress Syndrome (ARDS) is an illness that typically develops in people who are significantly ill or have serious injuries. ARDS is characterized by fluid build-up that occurs in the alveoli. T-cells are implicated as playing a role in the modulation of the aberrant response leadi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/5/825 |
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| author | Sara Hey Dayjah Whyte Minh-Chau Hoang Nick Le Joseph Natvig Claire Wingfield Charles Onyeama Judie Howrylak Inimary T. Toby |
| author_facet | Sara Hey Dayjah Whyte Minh-Chau Hoang Nick Le Joseph Natvig Claire Wingfield Charles Onyeama Judie Howrylak Inimary T. Toby |
| author_sort | Sara Hey |
| collection | DOAJ |
| description | Acute Respiratory Distress Syndrome (ARDS) is an illness that typically develops in people who are significantly ill or have serious injuries. ARDS is characterized by fluid build-up that occurs in the alveoli. T-cells are implicated as playing a role in the modulation of the aberrant response leading to excessive tissue damage and, eventually, ARDS. Complementarity Determining Region 3 (CDR3) sequences derived from T-cells are key players in the adaptive immune response. This response is governed by an elaborate specificity for distinct molecules and the ability to recognize and vigorously respond to repeated exposures to the same molecules. Most of the diversity in T-cell receptors (TCRs) is contained in the CDR3 regions of the heterodimeric cell-surface receptors. For this study, we employed the novel technology of immune sequencing to assess lung edema fluid. Our goal was to explore the landscape of CDR3 clonal sequences found within these samples. We obtained more than 3615 CDR3 sequences across samples in the study. Our data demonstrate that: (1) CDR3 sequences from lung edema fluid exhibit distinct clonal populations, and (2) CDR3 sequences can be further characterized based on biochemical features. Analysis of these CDR3 sequences offers insight into the CDR3-driven T-cell repertoire of ARDS. These findings represent the first step towards applications of this technology with these types of biological samples in the context of ARDS. |
| first_indexed | 2024-03-11T03:55:10Z |
| format | Article |
| id | doaj.art-da11b6cb405145cd8a0c7f9c8571e217 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T03:55:10Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-da11b6cb405145cd8a0c7f9c8571e2172023-11-18T00:39:44ZengMDPI AGBiomolecules2218-273X2023-05-0113582510.3390/biom13050825Analysis of CDR3 Sequences from T-Cell Receptor β in Acute Respiratory Distress SyndromeSara Hey0Dayjah Whyte1Minh-Chau Hoang2Nick Le3Joseph Natvig4Claire Wingfield5Charles Onyeama6Judie Howrylak7Inimary T. Toby8Department of Biology, University of Dallas, Irving, TX 75062, USADepartment of Biology, University of Dallas, Irving, TX 75062, USADepartment of Biology, University of Dallas, Irving, TX 75062, USADepartment of Biology, University of Dallas, Irving, TX 75062, USADepartment of Biology, University of Dallas, Irving, TX 75062, USADepartment of Biology, University of Dallas, Irving, TX 75062, USAT.C Pediatrics, Bedford, TX 76021, USAPulmonary, Allergy and Critical Care Division, Penn State Milton S. Hershey Medical Center, Hershey, PA 17033, USADepartment of Biology, University of Dallas, Irving, TX 75062, USAAcute Respiratory Distress Syndrome (ARDS) is an illness that typically develops in people who are significantly ill or have serious injuries. ARDS is characterized by fluid build-up that occurs in the alveoli. T-cells are implicated as playing a role in the modulation of the aberrant response leading to excessive tissue damage and, eventually, ARDS. Complementarity Determining Region 3 (CDR3) sequences derived from T-cells are key players in the adaptive immune response. This response is governed by an elaborate specificity for distinct molecules and the ability to recognize and vigorously respond to repeated exposures to the same molecules. Most of the diversity in T-cell receptors (TCRs) is contained in the CDR3 regions of the heterodimeric cell-surface receptors. For this study, we employed the novel technology of immune sequencing to assess lung edema fluid. Our goal was to explore the landscape of CDR3 clonal sequences found within these samples. We obtained more than 3615 CDR3 sequences across samples in the study. Our data demonstrate that: (1) CDR3 sequences from lung edema fluid exhibit distinct clonal populations, and (2) CDR3 sequences can be further characterized based on biochemical features. Analysis of these CDR3 sequences offers insight into the CDR3-driven T-cell repertoire of ARDS. These findings represent the first step towards applications of this technology with these types of biological samples in the context of ARDS.https://www.mdpi.com/2218-273X/13/5/825Complementarity Determining Region 3immune sequencingAcute Respiratory Distress SyndromeT-cell receptorsequence analysisbiochemical properties and clonality |
| spellingShingle | Sara Hey Dayjah Whyte Minh-Chau Hoang Nick Le Joseph Natvig Claire Wingfield Charles Onyeama Judie Howrylak Inimary T. Toby Analysis of CDR3 Sequences from T-Cell Receptor β in Acute Respiratory Distress Syndrome Biomolecules Complementarity Determining Region 3 immune sequencing Acute Respiratory Distress Syndrome T-cell receptor sequence analysis biochemical properties and clonality |
| title | Analysis of CDR3 Sequences from T-Cell Receptor β in Acute Respiratory Distress Syndrome |
| title_full | Analysis of CDR3 Sequences from T-Cell Receptor β in Acute Respiratory Distress Syndrome |
| title_fullStr | Analysis of CDR3 Sequences from T-Cell Receptor β in Acute Respiratory Distress Syndrome |
| title_full_unstemmed | Analysis of CDR3 Sequences from T-Cell Receptor β in Acute Respiratory Distress Syndrome |
| title_short | Analysis of CDR3 Sequences from T-Cell Receptor β in Acute Respiratory Distress Syndrome |
| title_sort | analysis of cdr3 sequences from t cell receptor β in acute respiratory distress syndrome |
| topic | Complementarity Determining Region 3 immune sequencing Acute Respiratory Distress Syndrome T-cell receptor sequence analysis biochemical properties and clonality |
| url | https://www.mdpi.com/2218-273X/13/5/825 |
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