Single-molecule analysis of steroid receptor and cofactor action in living cells
Transcription factors (TFs) are thought to regulate gene expression by stably binding to target DNA elements. Here, the authors use single-molecule tracking to analyse the dynamic behaviour of steroid receptors TFs and show that most specific interactions with chromatin are transient and dynamic.
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms15896 |
| _version_ | 1818749191485128704 |
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| author | Ville Paakinaho Diego M. Presman David A. Ball Thomas A. Johnson R. Louis Schiltz Peter Levitt Davide Mazza Tatsuya Morisaki Tatiana S. Karpova Gordon L. Hager |
| author_facet | Ville Paakinaho Diego M. Presman David A. Ball Thomas A. Johnson R. Louis Schiltz Peter Levitt Davide Mazza Tatsuya Morisaki Tatiana S. Karpova Gordon L. Hager |
| author_sort | Ville Paakinaho |
| collection | DOAJ |
| description | Transcription factors (TFs) are thought to regulate gene expression by stably binding to target DNA elements. Here, the authors use single-molecule tracking to analyse the dynamic behaviour of steroid receptors TFs and show that most specific interactions with chromatin are transient and dynamic. |
| first_indexed | 2024-12-18T03:59:51Z |
| format | Article |
| id | doaj.art-da11fc1b218545cd940439b7efb072c6 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-18T03:59:51Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-da11fc1b218545cd940439b7efb072c62022-12-21T21:21:43ZengNature PortfolioNature Communications2041-17232017-06-018111410.1038/ncomms15896Single-molecule analysis of steroid receptor and cofactor action in living cellsVille Paakinaho0Diego M. Presman1David A. Ball2Thomas A. Johnson3R. Louis Schiltz4Peter Levitt5Davide Mazza6Tatsuya Morisaki7Tatiana S. Karpova8Gordon L. Hager9Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthIstituto Scientifico Ospedale San Raffaele, Centro di Imaging Sperimentale e Università Vita-Salute San RaffaeleLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthLaboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of HealthTranscription factors (TFs) are thought to regulate gene expression by stably binding to target DNA elements. Here, the authors use single-molecule tracking to analyse the dynamic behaviour of steroid receptors TFs and show that most specific interactions with chromatin are transient and dynamic.https://doi.org/10.1038/ncomms15896 |
| spellingShingle | Ville Paakinaho Diego M. Presman David A. Ball Thomas A. Johnson R. Louis Schiltz Peter Levitt Davide Mazza Tatsuya Morisaki Tatiana S. Karpova Gordon L. Hager Single-molecule analysis of steroid receptor and cofactor action in living cells Nature Communications |
| title | Single-molecule analysis of steroid receptor and cofactor action in living cells |
| title_full | Single-molecule analysis of steroid receptor and cofactor action in living cells |
| title_fullStr | Single-molecule analysis of steroid receptor and cofactor action in living cells |
| title_full_unstemmed | Single-molecule analysis of steroid receptor and cofactor action in living cells |
| title_short | Single-molecule analysis of steroid receptor and cofactor action in living cells |
| title_sort | single molecule analysis of steroid receptor and cofactor action in living cells |
| url | https://doi.org/10.1038/ncomms15896 |
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