Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography
Berberidis radix, as a traditional Chinese herbal medicine, is well known as the main source of berberine. This herbal medicine has been used for the treatment of many diseases and alkaloids including jatrorrhizine, palmatine, and berberine are major bioactive constituents. Few literatures report th...
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Association of the Chemical Engineers of Serbia
2018-01-01
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| Series: | Chemical Industry and Chemical Engineering Quarterly |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/1451-9372/2018/1451-93721700041S.pdf |
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| author | Sun Changlei Zhao Wei Duan Wenjuan Yang Peng Yang Bingtian Jiang Jiaojiao Li Jia Wang Xiao |
| author_facet | Sun Changlei Zhao Wei Duan Wenjuan Yang Peng Yang Bingtian Jiang Jiaojiao Li Jia Wang Xiao |
| author_sort | Sun Changlei |
| collection | DOAJ |
| description | Berberidis radix, as a traditional Chinese herbal medicine, is well known as the main source of berberine. This herbal medicine has been used for the treatment of many diseases and alkaloids including jatrorrhizine, palmatine, and berberine are major bioactive constituents. Few literatures report the preparative separation of these alkaloids from Berberidis radix. In the present study, the combinative application of ultrasonic extraction and pH-zone-refining countercurrent chromatography was used to extract and purify these alkaloids. The PZRCCC separation was performed in a two-phase solvent system composed of chloroform– methanol–water (4:3:3 volume ratio), where 40 mM HCl was added in the upper aqueous stationary phase as a retainer and 10 mM triethylamine was added in the lower organic mobile phase as an eluter. From a crude sample of 1.5 g, palmatine (251.6 mg), berberine (392.5 mg), and jatrorrhizine (412.6 mg) were obtained in a single run with purities of all being over 93.0%, analyzed by HPLC. The chemical structures of the separated alkaloids were identified by electrospray ionization–mass spectrometry (ESI-MS), 1H-NMR and 13C-NMR. |
| first_indexed | 2024-04-13T03:42:58Z |
| format | Article |
| id | doaj.art-da133f720c024c6887f567b82ca306cb |
| institution | Directory Open Access Journal |
| issn | 1451-9372 2217-7434 |
| language | English |
| last_indexed | 2024-04-13T03:42:58Z |
| publishDate | 2018-01-01 |
| publisher | Association of the Chemical Engineers of Serbia |
| record_format | Article |
| series | Chemical Industry and Chemical Engineering Quarterly |
| spelling | doaj.art-da133f720c024c6887f567b82ca306cb2022-12-22T03:04:05ZengAssociation of the Chemical Engineers of SerbiaChemical Industry and Chemical Engineering Quarterly1451-93722217-74342018-01-012411710.2298/CICEQ151220041S1451-93721700041SPreparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatographySun Changlei0Zhao Wei1Duan Wenjuan2Yang Peng3Yang Bingtian4Jiang Jiaojiao5Li Jia6Wang Xiao7Shandong Academy of Sciences, Shandong Analysis and Test Center, Key Laboratory of TCM Quality Control Technology, Jinan, ChinaShandong Academy of Sciences, Shandong Analysis and Test Center, Key Laboratory of TCM Quality Control Technology, Jinan, ChinaShandong Academy of Sciences, Shandong Analysis and Test Center, Key Laboratory of TCM Quality Control Technology, Jinan, ChinaKangsen Sanfeng Biological Engineering Technology Co., Jinan, ChinaKangsen Sanfeng Biological Engineering Technology Co., Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong Academy of Sciences, Shandong Analysis and Test Center, Key Laboratory of TCM Quality Control Technology, Jinan, ChinaBerberidis radix, as a traditional Chinese herbal medicine, is well known as the main source of berberine. This herbal medicine has been used for the treatment of many diseases and alkaloids including jatrorrhizine, palmatine, and berberine are major bioactive constituents. Few literatures report the preparative separation of these alkaloids from Berberidis radix. In the present study, the combinative application of ultrasonic extraction and pH-zone-refining countercurrent chromatography was used to extract and purify these alkaloids. The PZRCCC separation was performed in a two-phase solvent system composed of chloroform– methanol–water (4:3:3 volume ratio), where 40 mM HCl was added in the upper aqueous stationary phase as a retainer and 10 mM triethylamine was added in the lower organic mobile phase as an eluter. From a crude sample of 1.5 g, palmatine (251.6 mg), berberine (392.5 mg), and jatrorrhizine (412.6 mg) were obtained in a single run with purities of all being over 93.0%, analyzed by HPLC. The chemical structures of the separated alkaloids were identified by electrospray ionization–mass spectrometry (ESI-MS), 1H-NMR and 13C-NMR.http://www.doiserbia.nb.rs/img/doi/1451-9372/2018/1451-93721700041S.pdfseparationpalmatineberberinejatrorrhizineBerberidis radixpH-zone-refining countercurrent chromatography |
| spellingShingle | Sun Changlei Zhao Wei Duan Wenjuan Yang Peng Yang Bingtian Jiang Jiaojiao Li Jia Wang Xiao Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography Chemical Industry and Chemical Engineering Quarterly separation palmatine berberine jatrorrhizine Berberidis radix pH-zone-refining countercurrent chromatography |
| title | Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography |
| title_full | Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography |
| title_fullStr | Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography |
| title_full_unstemmed | Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography |
| title_short | Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography |
| title_sort | preparative separation of three isoquinoline alkaloids from berberidis radix by ph zone refining countercurrent chromatography |
| topic | separation palmatine berberine jatrorrhizine Berberidis radix pH-zone-refining countercurrent chromatography |
| url | http://www.doiserbia.nb.rs/img/doi/1451-9372/2018/1451-93721700041S.pdf |
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