Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography

Berberidis radix, as a traditional Chinese herbal medicine, is well known as the main source of berberine. This herbal medicine has been used for the treatment of many diseases and alkaloids including jatrorrhizine, palmatine, and berberine are major bioactive constituents. Few literatures report th...

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Main Authors: Sun Changlei, Zhao Wei, Duan Wenjuan, Yang Peng, Yang Bingtian, Jiang Jiaojiao, Li Jia, Wang Xiao
Format: Article
Language:English
Published: Association of the Chemical Engineers of Serbia 2018-01-01
Series:Chemical Industry and Chemical Engineering Quarterly
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/1451-9372/2018/1451-93721700041S.pdf
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author Sun Changlei
Zhao Wei
Duan Wenjuan
Yang Peng
Yang Bingtian
Jiang Jiaojiao
Li Jia
Wang Xiao
author_facet Sun Changlei
Zhao Wei
Duan Wenjuan
Yang Peng
Yang Bingtian
Jiang Jiaojiao
Li Jia
Wang Xiao
author_sort Sun Changlei
collection DOAJ
description Berberidis radix, as a traditional Chinese herbal medicine, is well known as the main source of berberine. This herbal medicine has been used for the treatment of many diseases and alkaloids including jatrorrhizine, palmatine, and berberine are major bioactive constituents. Few literatures report the preparative separation of these alkaloids from Berberidis radix. In the present study, the combinative application of ultrasonic extraction and pH-zone-refining countercurrent chromatography was used to extract and purify these alkaloids. The PZRCCC separation was performed in a two-phase solvent system composed of chloroform– methanol–water (4:3:3 volume ratio), where 40 mM HCl was added in the upper aqueous stationary phase as a retainer and 10 mM triethylamine was added in the lower organic mobile phase as an eluter. From a crude sample of 1.5 g, palmatine (251.6 mg), berberine (392.5 mg), and jatrorrhizine (412.6 mg) were obtained in a single run with purities of all being over 93.0%, analyzed by HPLC. The chemical structures of the separated alkaloids were identified by electrospray ionization–mass spectrometry (ESI-MS), 1H-NMR and 13C-NMR.
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spelling doaj.art-da133f720c024c6887f567b82ca306cb2022-12-22T03:04:05ZengAssociation of the Chemical Engineers of SerbiaChemical Industry and Chemical Engineering Quarterly1451-93722217-74342018-01-012411710.2298/CICEQ151220041S1451-93721700041SPreparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatographySun Changlei0Zhao Wei1Duan Wenjuan2Yang Peng3Yang Bingtian4Jiang Jiaojiao5Li Jia6Wang Xiao7Shandong Academy of Sciences, Shandong Analysis and Test Center, Key Laboratory of TCM Quality Control Technology, Jinan, ChinaShandong Academy of Sciences, Shandong Analysis and Test Center, Key Laboratory of TCM Quality Control Technology, Jinan, ChinaShandong Academy of Sciences, Shandong Analysis and Test Center, Key Laboratory of TCM Quality Control Technology, Jinan, ChinaKangsen Sanfeng Biological Engineering Technology Co., Jinan, ChinaKangsen Sanfeng Biological Engineering Technology Co., Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong University of Traditional Chinese Medicine, Jinan, ChinaShandong Academy of Sciences, Shandong Analysis and Test Center, Key Laboratory of TCM Quality Control Technology, Jinan, ChinaBerberidis radix, as a traditional Chinese herbal medicine, is well known as the main source of berberine. This herbal medicine has been used for the treatment of many diseases and alkaloids including jatrorrhizine, palmatine, and berberine are major bioactive constituents. Few literatures report the preparative separation of these alkaloids from Berberidis radix. In the present study, the combinative application of ultrasonic extraction and pH-zone-refining countercurrent chromatography was used to extract and purify these alkaloids. The PZRCCC separation was performed in a two-phase solvent system composed of chloroform– methanol–water (4:3:3 volume ratio), where 40 mM HCl was added in the upper aqueous stationary phase as a retainer and 10 mM triethylamine was added in the lower organic mobile phase as an eluter. From a crude sample of 1.5 g, palmatine (251.6 mg), berberine (392.5 mg), and jatrorrhizine (412.6 mg) were obtained in a single run with purities of all being over 93.0%, analyzed by HPLC. The chemical structures of the separated alkaloids were identified by electrospray ionization–mass spectrometry (ESI-MS), 1H-NMR and 13C-NMR.http://www.doiserbia.nb.rs/img/doi/1451-9372/2018/1451-93721700041S.pdfseparationpalmatineberberinejatrorrhizineBerberidis radixpH-zone-refining countercurrent chromatography
spellingShingle Sun Changlei
Zhao Wei
Duan Wenjuan
Yang Peng
Yang Bingtian
Jiang Jiaojiao
Li Jia
Wang Xiao
Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography
Chemical Industry and Chemical Engineering Quarterly
separation
palmatine
berberine
jatrorrhizine
Berberidis radix
pH-zone-refining countercurrent chromatography
title Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography
title_full Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography
title_fullStr Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography
title_full_unstemmed Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography
title_short Preparative separation of three isoquinoline alkaloids from Berberidis radix by pH-zone-refining countercurrent chromatography
title_sort preparative separation of three isoquinoline alkaloids from berberidis radix by ph zone refining countercurrent chromatography
topic separation
palmatine
berberine
jatrorrhizine
Berberidis radix
pH-zone-refining countercurrent chromatography
url http://www.doiserbia.nb.rs/img/doi/1451-9372/2018/1451-93721700041S.pdf
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