Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation
Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion i...
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MDPI AG
2021-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/2/241 |
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| author | Magdalena Kurek Elisabet Åkesson Masahito Yoshihara Elizabeth Oliver Yanhua Cui Martin Becker João Pedro Alves-Lopes Ragnar Bjarnason Patrik Romerius Mikael Sundin Ulrika Norén Nyström Cecilia Langenskiöld Hartmut Vogt Lars Henningsohn Cecilia Petersen Olle Söder Jingtao Guo Rod T. Mitchell Kirsi Jahnukainen Jan-Bernd Stukenborg |
| author_facet | Magdalena Kurek Elisabet Åkesson Masahito Yoshihara Elizabeth Oliver Yanhua Cui Martin Becker João Pedro Alves-Lopes Ragnar Bjarnason Patrik Romerius Mikael Sundin Ulrika Norén Nyström Cecilia Langenskiöld Hartmut Vogt Lars Henningsohn Cecilia Petersen Olle Söder Jingtao Guo Rod T. Mitchell Kirsi Jahnukainen Jan-Bernd Stukenborg |
| author_sort | Magdalena Kurek |
| collection | DOAJ |
| description | Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions. |
| first_indexed | 2024-03-09T03:34:58Z |
| format | Article |
| id | doaj.art-da13d3212ab546fc9dd2f8ed9f73592d |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T03:34:58Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-da13d3212ab546fc9dd2f8ed9f73592d2023-12-03T14:51:04ZengMDPI AGCells2073-44092021-01-0110224110.3390/cells10020241Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility PreservationMagdalena Kurek0Elisabet Åkesson1Masahito Yoshihara2Elizabeth Oliver3Yanhua Cui4Martin Becker5João Pedro Alves-Lopes6Ragnar Bjarnason7Patrik Romerius8Mikael Sundin9Ulrika Norén Nyström10Cecilia Langenskiöld11Hartmut Vogt12Lars Henningsohn13Cecilia Petersen14Olle Söder15Jingtao Guo16Rod T. Mitchell17Kirsi Jahnukainen18Jan-Bernd Stukenborg19NORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, SwedenDivision of Neurogeriatrics, Department of Neurobiology Care Sciences & Society, Karolinska Institutet, 141 83 Huddinge, SwedenDepartment of Biosciences and Nutrition, Karolinska Institutet, 141 83 Huddinge, SwedenNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, SwedenNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, SwedenCenter of Neurodevelopmental Disorders (KIND), Department of Women’s and Children’s Health, Karolinska Institutet, Centre for Psychiatry Research, Region Stockholm and Astrid Lindgren Children’s Hospital, Karolinska University Hospital, 171 64 Solna, SwedenNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, SwedenChildren’s Medical Center, Landspítali University Hospital, 101 Reykjavik, IcelandDepartment of Paediatric Oncology and Haematology, Clinical Sciences, Lund University, Barn-och Ungdomssjukhuset Lund, Skånes Universitetssjukhus, 221 85 Lund, SwedenDivision of Paediatrics, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 141 52 Huddinge, SwedenDivision of Paediatrics, Department of Clinical Science, Umeå University, 901 87 Umeå, SwedenDepartment of Paediatric Oncology, The Queen Silvia Children’s Hospital, 416 50 Gothenburg, SwedenCrown Princess Victoria’s Child and Youth Hospital, and Department of Biomedical and Clinical Sciences, Linköping University, 581 83 Linköping, SwedenDivision of Urology, Institution for Clinical Science Intervention and Technology, Karolinska Institutet, 141 52 Huddinge, SwedenNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, SwedenNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, SwedenDivision of Urology, Department of Surgery, University of Utah School of Medicine, Salt Lake City, UT 84112, USAMRC Centre for Reproductive Health, The Queen’s Medical Research Institute, The University of Edinburgh, Edinburgh EH16 4TJ, UKNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, SwedenNORDFERTIL Research Lab Stockholm, Childhood Cancer Research Unit, Department of Women’s and Children’s Health, Karolinska Institutet, and Karolinska University Hospital, 171 64 Solna, SwedenFertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.https://www.mdpi.com/2073-4409/10/2/241germ cellsbasal membranestem cell nicheseminiferous tubulesinfertilitylate effects |
| spellingShingle | Magdalena Kurek Elisabet Åkesson Masahito Yoshihara Elizabeth Oliver Yanhua Cui Martin Becker João Pedro Alves-Lopes Ragnar Bjarnason Patrik Romerius Mikael Sundin Ulrika Norén Nyström Cecilia Langenskiöld Hartmut Vogt Lars Henningsohn Cecilia Petersen Olle Söder Jingtao Guo Rod T. Mitchell Kirsi Jahnukainen Jan-Bernd Stukenborg Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation Cells germ cells basal membrane stem cell niche seminiferous tubules infertility late effects |
| title | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
| title_full | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
| title_fullStr | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
| title_full_unstemmed | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
| title_short | Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation |
| title_sort | spermatogonia loss correlates with lama 1 expression in human prepubertal testes stored for fertility preservation |
| topic | germ cells basal membrane stem cell niche seminiferous tubules infertility late effects |
| url | https://www.mdpi.com/2073-4409/10/2/241 |
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