Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio Cholera
Background and purpose: Synthesis of homogeneous and equally important nanoparticles is very popular in various sciences. Preparation and use of gold nanoparticles is very important according to their applications in biotechnology. For synthesizing homogeneous gold nanoparticles many substrates and...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2019-12-01
|
| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-12963-en.html |
| _version_ | 1797944102574096384 |
|---|---|
| author | Naimeh Mahheidari Jamal Rashidiani Khadijeh Eskandari |
| author_facet | Naimeh Mahheidari Jamal Rashidiani Khadijeh Eskandari |
| author_sort | Naimeh Mahheidari |
| collection | DOAJ |
| description | Background and purpose: Synthesis of homogeneous and equally important nanoparticles is very popular in various sciences. Preparation and use of gold nanoparticles is very important according to their applications in biotechnology. For synthesizing homogeneous gold nanoparticles many substrates and nanostructures have been used. This study aimed at synthesis of gold nanoparticles by apoferritin to be conjugated to antibody for detection of vibrio cholera.
Materials and methods: In this study, gold nanoparticles were prepared in apoferritin and compared with apoferritin free gold nanoparticles. Data were characterized by UV-Vis, DLS, and FE-SEM spectroscopy.
Results: Findings showed that the synthesized gold nanoparticles in apoferritin are more homogeneous.
Conclusion: The distribution of synthesizing gold nanoparticle in presence of apoferritin was so homogenous. In the following step, gold nanoparticles were separated from apoferritin and conjugated to recombinant antibody for detecting vibrio cholera. By bacteria aatchment on gold nanoparticles, red shift ocured on wavelength, which was also visible to naked eyes. So, gold nanoparticles could be considered as a colored biosensor for detection of vibrio cholera. |
| first_indexed | 2024-04-10T20:33:21Z |
| format | Article |
| id | doaj.art-da14716e20dc42d2a384d71b897f6d5e |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:33:21Z |
| publishDate | 2019-12-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-da14716e20dc42d2a384d71b897f6d5e2023-01-25T05:07:55ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792019-12-01291794048Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio CholeraNaimeh Mahheidari0Jamal Rashidiani1Khadijeh Eskandari2 Phd Candidate of Tissue Engineering, Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran PhD of Nanobiotechnology, Nanobiotecnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Assistant Professor, Nanobiotecnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Background and purpose: Synthesis of homogeneous and equally important nanoparticles is very popular in various sciences. Preparation and use of gold nanoparticles is very important according to their applications in biotechnology. For synthesizing homogeneous gold nanoparticles many substrates and nanostructures have been used. This study aimed at synthesis of gold nanoparticles by apoferritin to be conjugated to antibody for detection of vibrio cholera. Materials and methods: In this study, gold nanoparticles were prepared in apoferritin and compared with apoferritin free gold nanoparticles. Data were characterized by UV-Vis, DLS, and FE-SEM spectroscopy. Results: Findings showed that the synthesized gold nanoparticles in apoferritin are more homogeneous. Conclusion: The distribution of synthesizing gold nanoparticle in presence of apoferritin was so homogenous. In the following step, gold nanoparticles were separated from apoferritin and conjugated to recombinant antibody for detecting vibrio cholera. By bacteria aatchment on gold nanoparticles, red shift ocured on wavelength, which was also visible to naked eyes. So, gold nanoparticles could be considered as a colored biosensor for detection of vibrio cholera.http://jmums.mazums.ac.ir/article-1-12963-en.htmlgold nanoparticlesapoferritinantibodyspectroscopy |
| spellingShingle | Naimeh Mahheidari Jamal Rashidiani Khadijeh Eskandari Gold Nanoparticle Synthesis by Apoferritin Conjugated-antibody in Detecting Vibrio Cholera Journal of Mazandaran University of Medical Sciences gold nanoparticles apoferritin antibody spectroscopy |
| title | Gold Nanoparticle Synthesis by Apoferritin
Conjugated-antibody in Detecting Vibrio Cholera |
| title_full | Gold Nanoparticle Synthesis by Apoferritin
Conjugated-antibody in Detecting Vibrio Cholera |
| title_fullStr | Gold Nanoparticle Synthesis by Apoferritin
Conjugated-antibody in Detecting Vibrio Cholera |
| title_full_unstemmed | Gold Nanoparticle Synthesis by Apoferritin
Conjugated-antibody in Detecting Vibrio Cholera |
| title_short | Gold Nanoparticle Synthesis by Apoferritin
Conjugated-antibody in Detecting Vibrio Cholera |
| title_sort | gold nanoparticle synthesis by apoferritin conjugated antibody in detecting vibrio cholera |
| topic | gold nanoparticles apoferritin antibody spectroscopy |
| url | http://jmums.mazums.ac.ir/article-1-12963-en.html |
| work_keys_str_mv | AT naimehmahheidari goldnanoparticlesynthesisbyapoferritinconjugatedantibodyindetectingvibriocholera AT jamalrashidiani goldnanoparticlesynthesisbyapoferritinconjugatedantibodyindetectingvibriocholera AT khadijeheskandari goldnanoparticlesynthesisbyapoferritinconjugatedantibodyindetectingvibriocholera |