Evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of Calotropis procera (Ait) against MCF-7 and SK-GT-4 cancer cell lines.
Alkaloids are regarded as important nitrogen-containing chemical compounds that serve as a rich source for discovering and developing new drugs where most plant-origin alkaloids have antiproliferation effects on different kinds of cancers. Alkaloids’ continence of Calotropis procera leaves are dete...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2023-10-01
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| Series: | Baghdad Science Journal |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7091 |
| _version_ | 1797669470896914432 |
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| author | Muna A.Y. Al-Mussawii Emad Y. A. AL-Sultan Maytham A. AL-Hamdani |
| author_facet | Muna A.Y. Al-Mussawii Emad Y. A. AL-Sultan Maytham A. AL-Hamdani |
| author_sort | Muna A.Y. Al-Mussawii |
| collection | DOAJ |
| description |
Alkaloids are regarded as important nitrogen-containing chemical compounds that serve as a rich source for discovering and developing new drugs where most plant-origin alkaloids have antiproliferation effects on different kinds of cancers. Alkaloids’ continence of Calotropis procera leaves are detected by two biochemical alkaloid reagents. Also GC-MS analysis for leaf alkaloid extract was done that showed the existence of one type of alkaloid compound at retention time12.8min detected as colchicine (C22H25N06( by comparing it with colchicine standard reference (Sigma Aldrich) with M.wt 399g/mol and percentage area 7.1%. Furthermore, identification, separation, and purification for purified colchicine compound were conducted by HPLC technique that gave one main peak at RT reached 2.5min compared with the standard reference. Evaluation of the anticancer activity of purified colchicine on two (MCF-7 &SK-GT-4) cell lines revealed significant cytotoxicity on the MCF-7 cell line that was superior to its cytotoxicity on the SK-GT4 cell line. With calculated IC50 reached 55.33µg/ml &522 µg/ml respectively.
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| first_indexed | 2024-03-11T20:44:51Z |
| format | Article |
| id | doaj.art-da18f025d2724ca5bbf40ef2518f5aa1 |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-03-11T20:44:51Z |
| publishDate | 2023-10-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-da18f025d2724ca5bbf40ef2518f5aa12023-10-01T19:55:46ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862023-10-0120510.21123/bsj.2023.7091Evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of Calotropis procera (Ait) against MCF-7 and SK-GT-4 cancer cell lines.Muna A.Y. Al-Mussawii0Emad Y. A. AL-Sultan1Maytham A. AL-Hamdani2Department of Pharmacognosy and Medicinal Plants, Pharmacy College, University of Basrah, Iraq.Department of Biology , College of Education for pure science, University of Basrah, Iraq.Department of Biology , College of Education for pure science, University of Basrah, Iraq. Alkaloids are regarded as important nitrogen-containing chemical compounds that serve as a rich source for discovering and developing new drugs where most plant-origin alkaloids have antiproliferation effects on different kinds of cancers. Alkaloids’ continence of Calotropis procera leaves are detected by two biochemical alkaloid reagents. Also GC-MS analysis for leaf alkaloid extract was done that showed the existence of one type of alkaloid compound at retention time12.8min detected as colchicine (C22H25N06( by comparing it with colchicine standard reference (Sigma Aldrich) with M.wt 399g/mol and percentage area 7.1%. Furthermore, identification, separation, and purification for purified colchicine compound were conducted by HPLC technique that gave one main peak at RT reached 2.5min compared with the standard reference. Evaluation of the anticancer activity of purified colchicine on two (MCF-7 &SK-GT-4) cell lines revealed significant cytotoxicity on the MCF-7 cell line that was superior to its cytotoxicity on the SK-GT4 cell line. With calculated IC50 reached 55.33µg/ml &522 µg/ml respectively. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7091Anticancer activity, Calotropis procera Leaf, Colchicine, Extraction and purification, GC-MS, HPLC |
| spellingShingle | Muna A.Y. Al-Mussawii Emad Y. A. AL-Sultan Maytham A. AL-Hamdani Evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of Calotropis procera (Ait) against MCF-7 and SK-GT-4 cancer cell lines. Baghdad Science Journal Anticancer activity, Calotropis procera Leaf, Colchicine, Extraction and purification, GC-MS, HPLC |
| title | Evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of Calotropis procera (Ait) against MCF-7 and SK-GT-4 cancer cell lines. |
| title_full | Evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of Calotropis procera (Ait) against MCF-7 and SK-GT-4 cancer cell lines. |
| title_fullStr | Evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of Calotropis procera (Ait) against MCF-7 and SK-GT-4 cancer cell lines. |
| title_full_unstemmed | Evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of Calotropis procera (Ait) against MCF-7 and SK-GT-4 cancer cell lines. |
| title_short | Evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of Calotropis procera (Ait) against MCF-7 and SK-GT-4 cancer cell lines. |
| title_sort | evaluation of the cytotoxic effects of the colchicine compound isolated from the leaves of calotropis procera ait against mcf 7 and sk gt 4 cancer cell lines |
| topic | Anticancer activity, Calotropis procera Leaf, Colchicine, Extraction and purification, GC-MS, HPLC |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7091 |
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