Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen Infection
Expression of eight different chitinase genes, representing members of five chitinase classes, was studied in Medicago truncatula roots during formation of arbuscular mycorrhiza with Glomus intraradices, nodulation with Rhizobium meliloti, and pathogen attack by Phytophthora megasperma f. sp. medica...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2000-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.7.763 |
| _version_ | 1818915198000431104 |
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| author | Peter Salzer Athos Bonanomi Katinka Beyer Regina Vögeli-Lange Roger A. Aeschbacher Jürg Lange Andres Wiemken Dongjin Kim Douglas R. Cook Thomas Boller |
| author_facet | Peter Salzer Athos Bonanomi Katinka Beyer Regina Vögeli-Lange Roger A. Aeschbacher Jürg Lange Andres Wiemken Dongjin Kim Douglas R. Cook Thomas Boller |
| author_sort | Peter Salzer |
| collection | DOAJ |
| description | Expression of eight different chitinase genes, representing members of five chitinase classes, was studied in Medicago truncatula roots during formation of arbuscular mycorrhiza with Glomus intraradices, nodulation with Rhizobium meliloti, and pathogen attack by Phytophthora megasperma f. sp. medicaginis, Fusarium solani f. sp. phaseoli (compatible interactions with root rot symptoms), Ascochyta pisi (compatible, symptomless), and F. solani f. sp. pisi (incompatible, nonhost interaction). In the compatible plant-pathogen interactions, expression of class I, II, and IV chitinase genes was enhanced. The same genes were induced during nodulation. Transcripts of class I and II chitinase genes accumulated transiently during early stages of the interaction, and transcripts of the class IV chitinase gene accumulated in mature nodules. The pattern of chitinase gene expression in mycorrhizal roots was markedly different: Expression of class I, II, and IV chitinase genes was not enhanced, whereas expression of three class III chitinase genes, with almost no basal expression, was strongly induced. Two of these three (Mtchitinase III-2 and Mtchitinase III-3) were not induced at all in interactions with pathogens and rhizobia. Thus, the expression of two mycorrhiza-specific class III chitinase genes can be considered a hallmark for the establishment of arbuscular mycorrhiza in Medicago truncatula. |
| first_indexed | 2024-12-19T23:58:28Z |
| format | Article |
| id | doaj.art-da1c8980da704f299e80c270b51d9308 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:58:28Z |
| publishDate | 2000-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-da1c8980da704f299e80c270b51d93082022-12-21T20:00:56ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-07-0113776377710.1094/MPMI.2000.13.7.763Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen InfectionPeter SalzerAthos BonanomiKatinka BeyerRegina Vögeli-LangeRoger A. AeschbacherJürg LangeAndres WiemkenDongjin KimDouglas R. CookThomas BollerExpression of eight different chitinase genes, representing members of five chitinase classes, was studied in Medicago truncatula roots during formation of arbuscular mycorrhiza with Glomus intraradices, nodulation with Rhizobium meliloti, and pathogen attack by Phytophthora megasperma f. sp. medicaginis, Fusarium solani f. sp. phaseoli (compatible interactions with root rot symptoms), Ascochyta pisi (compatible, symptomless), and F. solani f. sp. pisi (incompatible, nonhost interaction). In the compatible plant-pathogen interactions, expression of class I, II, and IV chitinase genes was enhanced. The same genes were induced during nodulation. Transcripts of class I and II chitinase genes accumulated transiently during early stages of the interaction, and transcripts of the class IV chitinase gene accumulated in mature nodules. The pattern of chitinase gene expression in mycorrhizal roots was markedly different: Expression of class I, II, and IV chitinase genes was not enhanced, whereas expression of three class III chitinase genes, with almost no basal expression, was strongly induced. Two of these three (Mtchitinase III-2 and Mtchitinase III-3) were not induced at all in interactions with pathogens and rhizobia. Thus, the expression of two mycorrhiza-specific class III chitinase genes can be considered a hallmark for the establishment of arbuscular mycorrhiza in Medicago truncatula.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.7.763chalcone synthasetrehalase |
| spellingShingle | Peter Salzer Athos Bonanomi Katinka Beyer Regina Vögeli-Lange Roger A. Aeschbacher Jürg Lange Andres Wiemken Dongjin Kim Douglas R. Cook Thomas Boller Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen Infection Molecular Plant-Microbe Interactions chalcone synthase trehalase |
| title | Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen Infection |
| title_full | Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen Infection |
| title_fullStr | Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen Infection |
| title_full_unstemmed | Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen Infection |
| title_short | Differential Expression of Eight Chitinase Genes in Medicago truncatula Roots During Mycorrhiza Formation, Nodulation, and Pathogen Infection |
| title_sort | differential expression of eight chitinase genes in medicago truncatula roots during mycorrhiza formation nodulation and pathogen infection |
| topic | chalcone synthase trehalase |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.7.763 |
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