Phylogenetic Analysis of bor Gene in an Escherichia coli Strain 1378 (O78:K80) Isolated from an Avian Colibacillosis Case in Tehran, Iran
Colibacillosis is known as a fatal bacterial disease resulting in a high level of commercial loss worldwide. This study amid to elucidate the sequence, genetic characteristics, and phylogeny of the <em>bor</em> gene in <em>Escherichia coli</em> (<em>E. coli</em>)...
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| Language: | English |
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Razi Vaccine and Serum Research Institute
2019-09-01
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| Series: | Archives of Razi Institute |
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| Online Access: | http://archrazi.areeo.ac.ir/article_119271_21ba07a4899628ee927d5ef641787537.pdf |
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| author | B. Nayeri Fasaei T. Zahraei Salehi S. Salari M. M. Ranjbar A. R. Yousefi |
| author_facet | B. Nayeri Fasaei T. Zahraei Salehi S. Salari M. M. Ranjbar A. R. Yousefi |
| author_sort | B. Nayeri Fasaei |
| collection | DOAJ |
| description | Colibacillosis is known as a fatal bacterial disease resulting in a high level of commercial loss worldwide. This study amid to elucidate the sequence, genetic characteristics, and phylogeny of the <em>bor</em> gene in <em>Escherichia coli</em> (<em>E. coli</em>) strain c1378 (O78:K80) isolated from avian colibacillosis in Iran and develop a rapid and optimal polymerase chain reaction (PCR) molecular-based technique with specific primers to detect this gene in <em>E. coli</em>. A virulent avian <em>E. coli</em> (i.e., laboratory designation <em>E. coli </em>strain c1378) isolated from a chicken with systemic colibacillosis from a broiler farm in Tehran, Iran, in 2004 was used as a source of the <em>bor </em>gene. After DNA extraction, PCR method was used to amplify the <em>bor</em> gene. A 658 bp fragment of the <em>bor</em> gene was amplified, sequenced, blasted, and phylogenetically studied. The most similar sequences to the <em>bor</em> gene in <em>E.</em> <em>coli</em> strain c1378 were <em>E. coli</em> APEC O78, Enterobacteria phage HK630, and <em>Escherichia coli</em> BW2952, respectively. There was a high similarity between the <em>bor</em> gene in <em>E. coli</em> bacteria with their phage and plasmid. Moreover, a high similarity was observed between the <em>bor</em> and <em>iss</em> genes (approximately 92%) showing that they were homologous genes. In addition, the similarity analysis of different bacterial species, as well as their plasmid and bacteriophage, to the <em>bor</em> gene indicated that the highest similarity to O78:K80 was related to <em>Paracoccidioides brasiliensis</em>, <em>Bacillus thuringiensis</em> CT43 plasmid pBMB0558, and <em>Salmonella enterica</em> subsp. enterica serovar Kentucky strain CVM29188 plasmid, respectively. Altogether, the results of the present study confirmed the presence of the <em>bor</em> gene in the studied isolates and clarified its sequence, phylogenetic relationship, and similarities of <em>E.</em> <em>coli</em> strain c1378 (O78:K80) isolated from avian colibacillosis. |
| first_indexed | 2024-12-11T10:43:01Z |
| format | Article |
| id | doaj.art-da20d06fcab84120847657192a9e9199 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-11T10:43:01Z |
| publishDate | 2019-09-01 |
| publisher | Razi Vaccine and Serum Research Institute |
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| series | Archives of Razi Institute |
| spelling | doaj.art-da20d06fcab84120847657192a9e91992022-12-22T01:10:32ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722019-09-0174331332010.22092/ari.2018.120869.1200119271Phylogenetic Analysis of bor Gene in an Escherichia coli Strain 1378 (O78:K80) Isolated from an Avian Colibacillosis Case in Tehran, IranB. Nayeri Fasaei0T. Zahraei Salehi1S. Salari2M. M. Ranjbar3A. R. Yousefi4Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, IranDepartment of FMD, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, IranDepartment of Pathology and Experimental Animals, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranColibacillosis is known as a fatal bacterial disease resulting in a high level of commercial loss worldwide. This study amid to elucidate the sequence, genetic characteristics, and phylogeny of the <em>bor</em> gene in <em>Escherichia coli</em> (<em>E. coli</em>) strain c1378 (O78:K80) isolated from avian colibacillosis in Iran and develop a rapid and optimal polymerase chain reaction (PCR) molecular-based technique with specific primers to detect this gene in <em>E. coli</em>. A virulent avian <em>E. coli</em> (i.e., laboratory designation <em>E. coli </em>strain c1378) isolated from a chicken with systemic colibacillosis from a broiler farm in Tehran, Iran, in 2004 was used as a source of the <em>bor </em>gene. After DNA extraction, PCR method was used to amplify the <em>bor</em> gene. A 658 bp fragment of the <em>bor</em> gene was amplified, sequenced, blasted, and phylogenetically studied. The most similar sequences to the <em>bor</em> gene in <em>E.</em> <em>coli</em> strain c1378 were <em>E. coli</em> APEC O78, Enterobacteria phage HK630, and <em>Escherichia coli</em> BW2952, respectively. There was a high similarity between the <em>bor</em> gene in <em>E. coli</em> bacteria with their phage and plasmid. Moreover, a high similarity was observed between the <em>bor</em> and <em>iss</em> genes (approximately 92%) showing that they were homologous genes. In addition, the similarity analysis of different bacterial species, as well as their plasmid and bacteriophage, to the <em>bor</em> gene indicated that the highest similarity to O78:K80 was related to <em>Paracoccidioides brasiliensis</em>, <em>Bacillus thuringiensis</em> CT43 plasmid pBMB0558, and <em>Salmonella enterica</em> subsp. enterica serovar Kentucky strain CVM29188 plasmid, respectively. Altogether, the results of the present study confirmed the presence of the <em>bor</em> gene in the studied isolates and clarified its sequence, phylogenetic relationship, and similarities of <em>E.</em> <em>coli</em> strain c1378 (O78:K80) isolated from avian colibacillosis.http://archrazi.areeo.ac.ir/article_119271_21ba07a4899628ee927d5ef641787537.pdfe. colicolibacillosisavianidentification |
| spellingShingle | B. Nayeri Fasaei T. Zahraei Salehi S. Salari M. M. Ranjbar A. R. Yousefi Phylogenetic Analysis of bor Gene in an Escherichia coli Strain 1378 (O78:K80) Isolated from an Avian Colibacillosis Case in Tehran, Iran Archives of Razi Institute e. coli colibacillosis avian identification |
| title | Phylogenetic Analysis of bor Gene in an Escherichia coli Strain 1378 (O78:K80) Isolated from an Avian Colibacillosis Case in Tehran, Iran |
| title_full | Phylogenetic Analysis of bor Gene in an Escherichia coli Strain 1378 (O78:K80) Isolated from an Avian Colibacillosis Case in Tehran, Iran |
| title_fullStr | Phylogenetic Analysis of bor Gene in an Escherichia coli Strain 1378 (O78:K80) Isolated from an Avian Colibacillosis Case in Tehran, Iran |
| title_full_unstemmed | Phylogenetic Analysis of bor Gene in an Escherichia coli Strain 1378 (O78:K80) Isolated from an Avian Colibacillosis Case in Tehran, Iran |
| title_short | Phylogenetic Analysis of bor Gene in an Escherichia coli Strain 1378 (O78:K80) Isolated from an Avian Colibacillosis Case in Tehran, Iran |
| title_sort | phylogenetic analysis of bor gene in an escherichia coli strain 1378 o78 k80 isolated from an avian colibacillosis case in tehran iran |
| topic | e. coli colibacillosis avian identification |
| url | http://archrazi.areeo.ac.ir/article_119271_21ba07a4899628ee927d5ef641787537.pdf |
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