The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method
Objective(s): A combination of biological and microemulsion methods was used to synthesize silver nanoparticles for the first time. The applied method could be referred to as the biomicroemulsion method, which has the advantages of both biological and the microemulsion methods.<br />Materials...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-07-01
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| Series: | Nanomedicine Journal |
| Subjects: | |
| Online Access: | http://nmj.mums.ac.ir/article_15643_741538c1f2306875f57ce3de8394e9e3.pdf |
| _version_ | 1818720371893862400 |
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| author | Hamid Reza Ghorbani Alireza Hoseinpour Kasgari Peyman Valipour Farnaz Jafari |
| author_facet | Hamid Reza Ghorbani Alireza Hoseinpour Kasgari Peyman Valipour Farnaz Jafari |
| author_sort | Hamid Reza Ghorbani |
| collection | DOAJ |
| description | Objective(s): A combination of biological and microemulsion methods was used to synthesize silver nanoparticles for the first time. The applied method could be referred to as the biomicroemulsion method, which has the advantages of both biological and the microemulsion methods.<br />Materials and Methods: In the present study, silver nanoparticles were synthesized in a water-in-oil biomicroemulsion using silver nitrate, which was solubilized in the water core of one microemulsion as the source of silver ions. In addition, a bacterial culture supernatant solubilized in the water core of another microemulsion was employed as the biological reducing agent, dodecane was used as the oil phase, and sodium bis(2-ethylhexyl) sulfosuccinate was applied as the surfactant. Moreover, the antibacterial activity of the nanoparticles was investigated against gram-positive and gram-negative bacteria by disc-diffusion method.<br />Results: The UV-Vis absorption spectra, dynamic light scattering, and transmission electron microscopy were employed to characterize the presence, size distribution, and morphology of the nanoparticles, respectively. According to the results, the nanoparticles had the optimal conditions in terms of the size and distribution at the silver nitrate concentration of 0.001 M. In addition, the analysis of antibacterial activity indicated that the inhibition zone diameter of Staphylococcus aureus was higher compared to Escherichia coli.<br />Conclusion: Silver nanoparticles were synthesized successfully using biomicroemulsion method and showed significant anti-bacterial activities against S. aureus and E. coli. |
| first_indexed | 2024-12-17T20:21:47Z |
| format | Article |
| id | doaj.art-da21957dff3140e5902de11572396913 |
| institution | Directory Open Access Journal |
| issn | 2322-3049 2322-5904 |
| language | English |
| last_indexed | 2024-12-17T20:21:47Z |
| publishDate | 2020-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj.art-da21957dff3140e5902de115723969132022-12-21T21:33:55ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042020-07-017323724210.22038/nmj.2020.07.000815643The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion methodHamid Reza Ghorbani0Alireza Hoseinpour Kasgari1Peyman Valipour2Farnaz Jafari3Department of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, IranDepartment of Textile Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, IranDepartment of Textile Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, IranDepartment of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, IranObjective(s): A combination of biological and microemulsion methods was used to synthesize silver nanoparticles for the first time. The applied method could be referred to as the biomicroemulsion method, which has the advantages of both biological and the microemulsion methods.<br />Materials and Methods: In the present study, silver nanoparticles were synthesized in a water-in-oil biomicroemulsion using silver nitrate, which was solubilized in the water core of one microemulsion as the source of silver ions. In addition, a bacterial culture supernatant solubilized in the water core of another microemulsion was employed as the biological reducing agent, dodecane was used as the oil phase, and sodium bis(2-ethylhexyl) sulfosuccinate was applied as the surfactant. Moreover, the antibacterial activity of the nanoparticles was investigated against gram-positive and gram-negative bacteria by disc-diffusion method.<br />Results: The UV-Vis absorption spectra, dynamic light scattering, and transmission electron microscopy were employed to characterize the presence, size distribution, and morphology of the nanoparticles, respectively. According to the results, the nanoparticles had the optimal conditions in terms of the size and distribution at the silver nitrate concentration of 0.001 M. In addition, the analysis of antibacterial activity indicated that the inhibition zone diameter of Staphylococcus aureus was higher compared to Escherichia coli.<br />Conclusion: Silver nanoparticles were synthesized successfully using biomicroemulsion method and showed significant anti-bacterial activities against S. aureus and E. coli.http://nmj.mums.ac.ir/article_15643_741538c1f2306875f57ce3de8394e9e3.pdfantibacterialbiomicroemulsionsynthesissilver nanoparticles |
| spellingShingle | Hamid Reza Ghorbani Alireza Hoseinpour Kasgari Peyman Valipour Farnaz Jafari The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method Nanomedicine Journal antibacterial biomicroemulsion synthesis silver nanoparticles |
| title | The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method |
| title_full | The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method |
| title_fullStr | The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method |
| title_full_unstemmed | The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method |
| title_short | The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method |
| title_sort | synthesis of silver nanoparticles using the water in oil biomicroemulsion method |
| topic | antibacterial biomicroemulsion synthesis silver nanoparticles |
| url | http://nmj.mums.ac.ir/article_15643_741538c1f2306875f57ce3de8394e9e3.pdf |
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