Chemical Constituents of <i>Callistemon subulatus</i> and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line
An n-hexane extract of <i>Callistemon subulatus</i> was found to exhibit potent cytotoxicity against PANC-1 human pancreatic cancer cells, preferentially under nutrition starvation conditions, with a PC<sub>50</sub> value of 6.2 µg/mL. Phytochemical investigation of this bioa...
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MDPI AG
2022-09-01
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| author | Juthamart Maneenet Ahmed M. Tawila Ashraf M. Omar Nguyen Duy Phan Chiharu Ojima Masahiro Kuroda Mao Sato Mio Mizoguchi Ikue Takahashi Suresh Awale |
| author_facet | Juthamart Maneenet Ahmed M. Tawila Ashraf M. Omar Nguyen Duy Phan Chiharu Ojima Masahiro Kuroda Mao Sato Mio Mizoguchi Ikue Takahashi Suresh Awale |
| author_sort | Juthamart Maneenet |
| collection | DOAJ |
| description | An n-hexane extract of <i>Callistemon subulatus</i> was found to exhibit potent cytotoxicity against PANC-1 human pancreatic cancer cells, preferentially under nutrition starvation conditions, with a PC<sub>50</sub> value of 6.2 µg/mL. Phytochemical investigation of this bioactive extract resulted in the isolation of fifteen compounds (<b>1</b>–<b>15</b>), including a new compound, subulatone A (–). The structure of compound <b>1</b> was elucidated using HRFABMS and NMR spectroscopic analyses. The isolated compounds were tested for their preferential cytotoxicity against the PANC-1 human pancreatic cancer cell line, using an anti-austerity strategy. Among these, myrtucommulone A (<b>2</b>) showed highly potent preferential cytotoxicity, with a PC<sub>50</sub> value of 0.28 µM. Myrtucommulone A (<b>2</b>) was found to alter PANC-1 cell morphology, inhibit cell migration, and downregulate the PI3K/Akt/mTOR and autophagy signaling pathways in nutrient-deprived media, leading to cancer cell death. Therefore, myrtucommulone A (<b>2</b>) is a lead compound for anticancer drug development based on an anti-austerity strategy. |
| first_indexed | 2024-03-09T21:18:17Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:18:17Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-da229ed3f9094358b1186c2fe088a1f52023-11-23T21:27:58ZengMDPI AGPlants2223-77472022-09-011119246610.3390/plants11192466Chemical Constituents of <i>Callistemon subulatus</i> and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell LineJuthamart Maneenet0Ahmed M. Tawila1Ashraf M. Omar2Nguyen Duy Phan3Chiharu Ojima4Masahiro Kuroda5Mao Sato6Mio Mizoguchi7Ikue Takahashi8Suresh Awale9Natural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanNatural Drug Discovery Laboratory, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, JapanAn n-hexane extract of <i>Callistemon subulatus</i> was found to exhibit potent cytotoxicity against PANC-1 human pancreatic cancer cells, preferentially under nutrition starvation conditions, with a PC<sub>50</sub> value of 6.2 µg/mL. Phytochemical investigation of this bioactive extract resulted in the isolation of fifteen compounds (<b>1</b>–<b>15</b>), including a new compound, subulatone A (–). The structure of compound <b>1</b> was elucidated using HRFABMS and NMR spectroscopic analyses. The isolated compounds were tested for their preferential cytotoxicity against the PANC-1 human pancreatic cancer cell line, using an anti-austerity strategy. Among these, myrtucommulone A (<b>2</b>) showed highly potent preferential cytotoxicity, with a PC<sub>50</sub> value of 0.28 µM. Myrtucommulone A (<b>2</b>) was found to alter PANC-1 cell morphology, inhibit cell migration, and downregulate the PI3K/Akt/mTOR and autophagy signaling pathways in nutrient-deprived media, leading to cancer cell death. Therefore, myrtucommulone A (<b>2</b>) is a lead compound for anticancer drug development based on an anti-austerity strategy.https://www.mdpi.com/2223-7747/11/19/2466preferential cytotoxicitypancreatic canceranti-austerity<i>Callistemon subulatus</i> |
| spellingShingle | Juthamart Maneenet Ahmed M. Tawila Ashraf M. Omar Nguyen Duy Phan Chiharu Ojima Masahiro Kuroda Mao Sato Mio Mizoguchi Ikue Takahashi Suresh Awale Chemical Constituents of <i>Callistemon subulatus</i> and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line Plants preferential cytotoxicity pancreatic cancer anti-austerity <i>Callistemon subulatus</i> |
| title | Chemical Constituents of <i>Callistemon subulatus</i> and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line |
| title_full | Chemical Constituents of <i>Callistemon subulatus</i> and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line |
| title_fullStr | Chemical Constituents of <i>Callistemon subulatus</i> and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line |
| title_full_unstemmed | Chemical Constituents of <i>Callistemon subulatus</i> and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line |
| title_short | Chemical Constituents of <i>Callistemon subulatus</i> and Their Anti-Pancreatic Cancer Activity against Human PANC-1 Cell Line |
| title_sort | chemical constituents of i callistemon subulatus i and their anti pancreatic cancer activity against human panc 1 cell line |
| topic | preferential cytotoxicity pancreatic cancer anti-austerity <i>Callistemon subulatus</i> |
| url | https://www.mdpi.com/2223-7747/11/19/2466 |
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