Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages
Objectives: l-dopa decarboxylase (DDC) is responsible for the synthesis of dopamine. Dopamine, which binds to the D2-dopamine receptor (D2R), plays an important role in the maintenance of pregnancy. Aim of our study was the analysis of DDC and D2R expression in placentas of spontaneous miscarriages...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bioscientifica
2018-05-01
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| Series: | Endocrine Connections |
| Subjects: | |
| Online Access: | http://www.endocrineconnections.com/content/7/5/727.full |
| _version_ | 1819068910312357888 |
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| author | Michael J Gratz Stavroula Stavrou Christina Kuhn Simone Hofmann Kerstin Hermelink Helene Heidegger Stefan Hutter Doris Mayr Sven Mahner Udo Jeschke Aurelia Vattai |
| author_facet | Michael J Gratz Stavroula Stavrou Christina Kuhn Simone Hofmann Kerstin Hermelink Helene Heidegger Stefan Hutter Doris Mayr Sven Mahner Udo Jeschke Aurelia Vattai |
| author_sort | Michael J Gratz |
| collection | DOAJ |
| description | Objectives: l-dopa decarboxylase (DDC) is responsible for the synthesis of dopamine. Dopamine, which binds to the D2-dopamine receptor (D2R), plays an important role in the maintenance of pregnancy. Aim of our study was the analysis of DDC and D2R expression in placentas of spontaneous miscarriages (SMs) and recurrent miscarriages (RMs) in comparison to healthy controls.
Methods: Patients with SM (n = 15) and RM (n = 15) were compared with patients from healthy pregnancies (n = 15) (pregnancy weeks 7–13 each). Placental tissue has been collected from SMs and RMs from the first trimester (Department of Gynaecology and Obstetrics, LMU Munich) and from abruptions (private practice, Munich). Placental cell lines, BeWo- and JEG-3 cells, were stimulated with the trace amines T0AM and T1AM in vitro.
Results: Levels of DDC and D2R in trophoblasts and the decidua were lower in RMs in comparison to healthy controls. Stimulation of BeWo cells with T1AM significantly reduced DDC mRNA and protein levels. Via double-immunofluorescence, a DDC-positive cell type beneath decidual stromal cells and foetal EVT in the decidua could be detected.
Conclusions: Downregulation of DDC and D2R in trophoblasts of RMs reflects a reduced signal cascade of catecholamines on the foetal side. |
| first_indexed | 2024-12-21T16:41:39Z |
| format | Article |
| id | doaj.art-da233839ddad4b64b19d1b25a5f3f963 |
| institution | Directory Open Access Journal |
| issn | 2049-3614 2049-3614 |
| language | English |
| last_indexed | 2024-12-21T16:41:39Z |
| publishDate | 2018-05-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Endocrine Connections |
| spelling | doaj.art-da233839ddad4b64b19d1b25a5f3f9632022-12-21T18:57:05ZengBioscientificaEndocrine Connections2049-36142049-36142018-05-0175727738https://doi.org/10.1530/EC-18-0126Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriagesMichael J Gratz0Stavroula Stavrou1Christina Kuhn2Simone Hofmann3Kerstin Hermelink4Helene Heidegger5Stefan Hutter6Doris Mayr7Sven Mahner8Udo Jeschke9Aurelia Vattai10Department of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Pathology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyDepartment of Obstetrics and Gynecology, University Hospital, LMU Munich, Munich, GermanyObjectives: l-dopa decarboxylase (DDC) is responsible for the synthesis of dopamine. Dopamine, which binds to the D2-dopamine receptor (D2R), plays an important role in the maintenance of pregnancy. Aim of our study was the analysis of DDC and D2R expression in placentas of spontaneous miscarriages (SMs) and recurrent miscarriages (RMs) in comparison to healthy controls. Methods: Patients with SM (n = 15) and RM (n = 15) were compared with patients from healthy pregnancies (n = 15) (pregnancy weeks 7–13 each). Placental tissue has been collected from SMs and RMs from the first trimester (Department of Gynaecology and Obstetrics, LMU Munich) and from abruptions (private practice, Munich). Placental cell lines, BeWo- and JEG-3 cells, were stimulated with the trace amines T0AM and T1AM in vitro. Results: Levels of DDC and D2R in trophoblasts and the decidua were lower in RMs in comparison to healthy controls. Stimulation of BeWo cells with T1AM significantly reduced DDC mRNA and protein levels. Via double-immunofluorescence, a DDC-positive cell type beneath decidual stromal cells and foetal EVT in the decidua could be detected. Conclusions: Downregulation of DDC and D2R in trophoblasts of RMs reflects a reduced signal cascade of catecholamines on the foetal side.http://www.endocrineconnections.com/content/7/5/727.fulll-dopa-decarboxylasedopamine D2L receptorthyronamine3-iodothyronaminemiscarriages |
| spellingShingle | Michael J Gratz Stavroula Stavrou Christina Kuhn Simone Hofmann Kerstin Hermelink Helene Heidegger Stefan Hutter Doris Mayr Sven Mahner Udo Jeschke Aurelia Vattai Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages Endocrine Connections l-dopa-decarboxylase dopamine D2L receptor thyronamine 3-iodothyronamine miscarriages |
| title | Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages |
| title_full | Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages |
| title_fullStr | Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages |
| title_full_unstemmed | Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages |
| title_short | Dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages |
| title_sort | dopamine synthesis and dopamine receptor expression are disturbed in recurrent miscarriages |
| topic | l-dopa-decarboxylase dopamine D2L receptor thyronamine 3-iodothyronamine miscarriages |
| url | http://www.endocrineconnections.com/content/7/5/727.full |
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