Development of UV-spectrophotometry Method of the Quantitative Determination of a New Substance Quinazoline-4(3h)-on Derivate
Introduction. The aim idea of this research article is a development of the quantitative determination of a biologically active substance quinazolin-4(3H)-on derivate with laboratory cypher «VMA-10-182, by UV-spectrophotometry with followed validation. The substance is an effective remedy that combi...
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| Format: | Article |
| Language: | Russian |
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LLC Center of Pharmaceutical Analytics (LLC «CPHA»)
2020-02-01
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| Series: | Разработка и регистрация лекарственных средств |
| Subjects: | |
| Online Access: | https://www.pharmjournal.ru/jour/article/view/726 |
| _version_ | 1797874993573396480 |
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| author | E. S. Mishchenko A. D. Lazaryan T. T. Lihota |
| author_facet | E. S. Mishchenko A. D. Lazaryan T. T. Lihota |
| author_sort | E. S. Mishchenko |
| collection | DOAJ |
| description | Introduction. The aim idea of this research article is a development of the quantitative determination of a biologically active substance quinazolin-4(3H)-on derivate with laboratory cypher «VMA-10-182, by UV-spectrophotometry with followed validation. The substance is an effective remedy that combines several pharmacological effects, like an antidepressant, anxiolytic and nootropic. As a result of preclinical trials, the research compound has proven to be an effective remedy in the fight and prevention of acute cerebrovascular accident (stroke). The substance realized pharmacological effects by stimulating the production of nitric oxide by the endothelial cells of the brain. As aresult of stimulating is a vasodilation of the vessels and improvement of blood flow in the ischemic part of the vessels occur. Therefore, for introducing the biologically active substance into medical practice we need to develop ways to control the quality of substance.Aim. The objective of this research work is to develop a method of the quantitative determination of a biologically active substance, derivative quinazolin-4(3H)-on (laboratory sypher – VMA-10-18), by method of UV spectrophotometry. The results of the research work were validated.Materials and methods. In this research we used a substance VMA-10-18 wich was previously purified from the initial and intermediate products of the synthesis. This substance is a white crystalline powder, odorless, hygroscopic.Results and discussion. The quantitative content of the active substance derived quinazolin-4(3H)-on has been determined. The specific absorption rate was calculated, followed by statistical processing of the results. The validation was carried out according to the «Specificity», «Linearity», «Accuracy», «Repeatability». The results indicate the effectiveness of the developed methodology and experimental reproducibility.Conclusion. Researches of physicochemical properties show al us use 95 % ethanol as a solvent. As a result we developed a method for the quantitative determination of the substance which can be proposed for inclusion in the normative documents. The quantitative determination of the active substance in the test substance was established, and the specific absorption index was calculated. All information are statistically processed and meet the requirements of regulatory documentation. |
| first_indexed | 2024-04-10T01:40:43Z |
| format | Article |
| id | doaj.art-da25bc87bc294ebd9e0131b80c537a89 |
| institution | Directory Open Access Journal |
| issn | 2305-2066 2658-5049 |
| language | Russian |
| last_indexed | 2024-04-10T01:40:43Z |
| publishDate | 2020-02-01 |
| publisher | LLC Center of Pharmaceutical Analytics (LLC «CPHA») |
| record_format | Article |
| series | Разработка и регистрация лекарственных средств |
| spelling | doaj.art-da25bc87bc294ebd9e0131b80c537a892023-03-13T09:14:00ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492020-02-0191303410.33380/2305-2066-2020-9-1-30-34711Development of UV-spectrophotometry Method of the Quantitative Determination of a New Substance Quinazoline-4(3h)-on DerivateE. S. Mishchenko0A. D. Lazaryan1T. T. Lihota2Пятигорский медико-фармацевтический институт, филиал ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения РоссииПятигорский медико-фармацевтический институт, филиал ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения РоссииПятигорский медико-фармацевтический институт, филиал ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения РоссииIntroduction. The aim idea of this research article is a development of the quantitative determination of a biologically active substance quinazolin-4(3H)-on derivate with laboratory cypher «VMA-10-182, by UV-spectrophotometry with followed validation. The substance is an effective remedy that combines several pharmacological effects, like an antidepressant, anxiolytic and nootropic. As a result of preclinical trials, the research compound has proven to be an effective remedy in the fight and prevention of acute cerebrovascular accident (stroke). The substance realized pharmacological effects by stimulating the production of nitric oxide by the endothelial cells of the brain. As aresult of stimulating is a vasodilation of the vessels and improvement of blood flow in the ischemic part of the vessels occur. Therefore, for introducing the biologically active substance into medical practice we need to develop ways to control the quality of substance.Aim. The objective of this research work is to develop a method of the quantitative determination of a biologically active substance, derivative quinazolin-4(3H)-on (laboratory sypher – VMA-10-18), by method of UV spectrophotometry. The results of the research work were validated.Materials and methods. In this research we used a substance VMA-10-18 wich was previously purified from the initial and intermediate products of the synthesis. This substance is a white crystalline powder, odorless, hygroscopic.Results and discussion. The quantitative content of the active substance derived quinazolin-4(3H)-on has been determined. The specific absorption rate was calculated, followed by statistical processing of the results. The validation was carried out according to the «Specificity», «Linearity», «Accuracy», «Repeatability». The results indicate the effectiveness of the developed methodology and experimental reproducibility.Conclusion. Researches of physicochemical properties show al us use 95 % ethanol as a solvent. As a result we developed a method for the quantitative determination of the substance which can be proposed for inclusion in the normative documents. The quantitative determination of the active substance in the test substance was established, and the specific absorption index was calculated. All information are statistically processed and meet the requirements of regulatory documentation.https://www.pharmjournal.ru/jour/article/view/726хиназолин-4(3н)-онсубстанция vma-10-18уф-спектрофотометриявалидацияострые нарушения мозгового кровообращения |
| spellingShingle | E. S. Mishchenko A. D. Lazaryan T. T. Lihota Development of UV-spectrophotometry Method of the Quantitative Determination of a New Substance Quinazoline-4(3h)-on Derivate Разработка и регистрация лекарственных средств хиназолин-4(3н)-он субстанция vma-10-18 уф-спектрофотометрия валидация острые нарушения мозгового кровообращения |
| title | Development of UV-spectrophotometry Method of the Quantitative Determination of a New Substance Quinazoline-4(3h)-on Derivate |
| title_full | Development of UV-spectrophotometry Method of the Quantitative Determination of a New Substance Quinazoline-4(3h)-on Derivate |
| title_fullStr | Development of UV-spectrophotometry Method of the Quantitative Determination of a New Substance Quinazoline-4(3h)-on Derivate |
| title_full_unstemmed | Development of UV-spectrophotometry Method of the Quantitative Determination of a New Substance Quinazoline-4(3h)-on Derivate |
| title_short | Development of UV-spectrophotometry Method of the Quantitative Determination of a New Substance Quinazoline-4(3h)-on Derivate |
| title_sort | development of uv spectrophotometry method of the quantitative determination of a new substance quinazoline 4 3h on derivate |
| topic | хиназолин-4(3н)-он субстанция vma-10-18 уф-спектрофотометрия валидация острые нарушения мозгового кровообращения |
| url | https://www.pharmjournal.ru/jour/article/view/726 |
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