Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts
The research on compounds exhibiting photoprotection against ultraviolet radiation (UVR) is a matter of increasing interest. The methanolic extract of a cell culture of <i>Buddleja cordata</i> has potential photoprotective effects as these cells produce phenolic secondary metabolites (SM...
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MDPI AG
2021-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/2/266 |
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| author | Milton Abraham Gómez-Hernández Miriam V. Flores-Merino Jesús Enrique Sánchez-Flores Cristina Burrola-Aguilar Carmen Zepeda-Gómez Aurelio Nieto-Trujillo María Elena Estrada-Zúñiga |
| author_facet | Milton Abraham Gómez-Hernández Miriam V. Flores-Merino Jesús Enrique Sánchez-Flores Cristina Burrola-Aguilar Carmen Zepeda-Gómez Aurelio Nieto-Trujillo María Elena Estrada-Zúñiga |
| author_sort | Milton Abraham Gómez-Hernández |
| collection | DOAJ |
| description | The research on compounds exhibiting photoprotection against ultraviolet radiation (UVR) is a matter of increasing interest. The methanolic extract of a cell culture of <i>Buddleja cordata</i> has potential photoprotective effects as these cells produce phenolic secondary metabolites (SMs). These metabolites are attributed with biological activities capable of counteracting the harmful effects caused by UVR on skin. In the present work, the methanolic extract (310–2500 µg/mL) of <i>B. cordata</i> cell culture showed a photoprotective effect on UVB-irradiated 3T3-Swiss albino fibroblasts with a significant increase in cell viability. The greatest photoprotective effect (75%) of the extract was observed at 2500 µg/mL, which was statistically comparable with that of 250 µg/mL verbascoside, used as positive control. In addition, concentrations of the extract higher than 2500 µg/mL resulted in decreased cell viability (≤83%) after 24 h of exposure. Phytochemical analysis of the extract allowed us to determine that it was characterized by high concentrations of total phenol and total phenolic acid contents (138 ± 4.7 mg gallic acid equivalents and 44.01 ± 1.33 mg verbascoside equivalents per gram of extract, respectively) as well as absorption of UV light (first and second bands peaking at 294 and 330 nm, respectively). Some phenylethanoid glycosides were identified from the extract. |
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| last_indexed | 2024-03-09T03:16:25Z |
| publishDate | 2021-01-01 |
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| series | Plants |
| spelling | doaj.art-da2964e90aa44c4788f1f4ddb24922ab2023-12-03T15:21:45ZengMDPI AGPlants2223-77472021-01-0110226610.3390/plants10020266Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino FibroblastsMilton Abraham Gómez-Hernández0Miriam V. Flores-Merino1Jesús Enrique Sánchez-Flores2Cristina Burrola-Aguilar3Carmen Zepeda-Gómez4Aurelio Nieto-Trujillo5María Elena Estrada-Zúñiga6Laboratorio de Toxicología de Productos Naturales, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (ENCB-IPN), Av. Wilfrido Massieu, Esq. Con Manuel M. Stampa s/n, Colonia Planetario Lindavista, Delegación Gustavo A. Madero, Ciudad de México C.P. 07700, MexicoFacultad de Química, Universidad Autónoma del Estado de México, Paseo Colón s/n, Residencial Colón y Col Ciprés, Toluca C.P. 50120, MexicoFacultad de Química, Universidad Autónoma del Estado de México, Paseo Colón s/n, Residencial Colón y Col Ciprés, Toluca C.P. 50120, MexicoCentro de Investigación en Recursos Bióticos-Facultad de Ciencias, Universidad Autónoma del Estado de México, Carretera Toluca-Ixtlahuaca Km 14.5, San Cayetano, Toluca C.P. 50295, MexicoFacultad de Ciencias, Universidad Autónoma del Estado de México, Campus El Cerrillo, Piedras Blancas, Carretera Toluca-Ixtlahuaca Km. 15.5, Toluca C.P. 50200, MexicoCentro de Investigación en Recursos Bióticos-Facultad de Ciencias, Universidad Autónoma del Estado de México, Carretera Toluca-Ixtlahuaca Km 14.5, San Cayetano, Toluca C.P. 50295, MexicoCentro de Investigación en Recursos Bióticos-Facultad de Ciencias, Universidad Autónoma del Estado de México, Carretera Toluca-Ixtlahuaca Km 14.5, San Cayetano, Toluca C.P. 50295, MexicoThe research on compounds exhibiting photoprotection against ultraviolet radiation (UVR) is a matter of increasing interest. The methanolic extract of a cell culture of <i>Buddleja cordata</i> has potential photoprotective effects as these cells produce phenolic secondary metabolites (SMs). These metabolites are attributed with biological activities capable of counteracting the harmful effects caused by UVR on skin. In the present work, the methanolic extract (310–2500 µg/mL) of <i>B. cordata</i> cell culture showed a photoprotective effect on UVB-irradiated 3T3-Swiss albino fibroblasts with a significant increase in cell viability. The greatest photoprotective effect (75%) of the extract was observed at 2500 µg/mL, which was statistically comparable with that of 250 µg/mL verbascoside, used as positive control. In addition, concentrations of the extract higher than 2500 µg/mL resulted in decreased cell viability (≤83%) after 24 h of exposure. Phytochemical analysis of the extract allowed us to determine that it was characterized by high concentrations of total phenol and total phenolic acid contents (138 ± 4.7 mg gallic acid equivalents and 44.01 ± 1.33 mg verbascoside equivalents per gram of extract, respectively) as well as absorption of UV light (first and second bands peaking at 294 and 330 nm, respectively). Some phenylethanoid glycosides were identified from the extract.https://www.mdpi.com/2223-7747/10/2/266<i>Buddleja cordata</i>phenolic secondary metabolitesterpene secondary metabolitesUV radiationphotoprotectioncytotoxicity |
| spellingShingle | Milton Abraham Gómez-Hernández Miriam V. Flores-Merino Jesús Enrique Sánchez-Flores Cristina Burrola-Aguilar Carmen Zepeda-Gómez Aurelio Nieto-Trujillo María Elena Estrada-Zúñiga Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts Plants <i>Buddleja cordata</i> phenolic secondary metabolites terpene secondary metabolites UV radiation photoprotection cytotoxicity |
| title | Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts |
| title_full | Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts |
| title_fullStr | Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts |
| title_full_unstemmed | Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts |
| title_short | Photoprotective Activity of <i>Buddleja cordata</i> Cell Culture Methanolic Extract on UVB-irradiated 3T3-Swiss Albino Fibroblasts |
| title_sort | photoprotective activity of i buddleja cordata i cell culture methanolic extract on uvb irradiated 3t3 swiss albino fibroblasts |
| topic | <i>Buddleja cordata</i> phenolic secondary metabolites terpene secondary metabolites UV radiation photoprotection cytotoxicity |
| url | https://www.mdpi.com/2223-7747/10/2/266 |
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